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公开(公告)号:AU2015277857B2
公开(公告)日:2021-07-22
申请号:AU2015277857
申请日:2015-06-18
Applicant: KAO CORP
Inventor: OZAKI TATSURO , SAITO TAKESHI , OZAWA TADAHIRO
Abstract: A method for manufacturing a lipid, the method comprising culturing a transgenic organism obtained by introducing a gene coding for any one protein selected from (A) through (C) into a host, and causing the transgenic organism to produce the lipid. (A) A protein comprising the amino acid sequence represented by positions 128 through 287 of SEQ ID NO: 1, wherein glycine at position 203 and/or valine at position 204 of the amino acid sequence is replaced with tryptophan. (B) A protein comprising an amino acid sequence having 85% or higher identity with the amino acid sequence represented by positions 128 through 287 of SEQ ID NO: 1, wherein the amino acid in the position corresponding to position 203 and/or the amino acid in the position corresponding to position 204 of SEQ ID NO: 1 is replaced with trypotophan, the protein also having acyl-ACP thioesterase activity. (C) A protein having the amino acid sequence of protein (A) or (B) and having acyl-ACP thioesterase activity.
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公开(公告)号:AU2015277857A1
公开(公告)日:2017-01-12
申请号:AU2015277857
申请日:2015-06-18
Applicant: KAO CORP
Inventor: OZAKI TATSURO , SAITO TAKESHI , OZAWA TADAHIRO
Abstract: A method for manufacturing a lipid, the method comprising culturing a transgenic organism obtained by introducing a gene coding for any one protein selected from (A) through (C) into a host, and causing the transgenic organism to produce the lipid. (A) A protein comprising the amino acid sequence represented by positions 128 through 287 of SEQ ID NO: 1, wherein glycine at position 203 and/or valine at position 204 of the amino acid sequence is replaced with tryptophan. (B) A protein comprising an amino acid sequence having 85% or higher identity with the amino acid sequence represented by positions 128 through 287 of SEQ ID NO: 1, wherein the amino acid in the position corresponding to position 203 and/or the amino acid in the position corresponding to position 204 of SEQ ID NO: 1 is replaced with trypotophan, the protein also having acyl-ACP thioesterase activity. (C) A protein having the amino acid sequence of protein (A) or (B) and having acyl-ACP thioesterase activity.
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公开(公告)号:DK1498476T3
公开(公告)日:2009-01-26
申请号:DK03719212
申请日:2003-04-25
Applicant: KAO CORP
Inventor: HAKAMADA YOSHIHIRO , OZAWA TADAHIRO , KOBAYASHI TOORU
Abstract: The present invention is directed to a mutated alkaline cellulase obtained by deleting, from a cellulase having an amino acid sequence represented by SEQ ID NO: 1 or an amino acid sequence exhibiting at least 90% homology therewith, one or more amino acid residue(s) chosen from the 343rd to 377th positions in SEQ ID NO: 1 or from corresponding positions and inserting a peptide having 2 to 15 amino acid residues into at least one of the deleted positions; and to a gene encoding the mutated alkaline cellulase. The mutated alkaline cellulase has an optimum pH near the pH of the washing liquid (pH: about 10.5) and thus is useful as an enzyme for laundry detergent.
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公开(公告)号:EP1391502A4
公开(公告)日:2005-01-26
申请号:EP02728174
申请日:2002-05-28
Applicant: KAO CORP
Inventor: SAWADA KAZUHISA , ENDO KEIJI , OZAWA TADAHIRO , TOHATA MASATOSHI , OZAKI KATSUYA
CPC classification number: C07K14/32 , C07K2319/00 , C12N1/20
Abstract: A microorganism wherein one or more genes selected from among a group of genes participating in sporulation in the medium to latter stages of sporulation have been deleted or inactivated; and a process for producing a target product (a protein) using the microorganism. No spore is formed by using this microorganism, which makes it possible to produce a target product (a protein) while relieving energy loss, suppressing the production of a by−product and a decrease in the specific production speed, and largely saving unnecessary consumption of a medium. Moreover, the production period can be prolonged thereby and thus the target product (the protein) can be efficiently produced.
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公开(公告)号:DK1391502T3
公开(公告)日:2012-05-21
申请号:DK02728174
申请日:2002-05-28
Applicant: KAO CORP
Inventor: SAWADA KAZUHISA , ENDO KEIJI , OZAWA TADAHIRO , TOHATA MASATOSHI , OZAKI KATSUYA
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公开(公告)号:DE69733524T2
公开(公告)日:2006-05-11
申请号:DE69733524
申请日:1997-09-29
Applicant: KAO CORP
Inventor: NISHIOKA TOHRU , TANAKA SHIGEYOSHI , KOSHINO JUNJI , YAMASHITA OSAMU , OZAWA TADAHIRO , KOHAMA MAKOTO
IPC: C07D309/18
Abstract: To provide a simple process for economically preparing a dihydropyrane compound at a high productivity and a high reaction yield. To use a compound selected from the group consisting of a base and a compound (VIII) having a weaker coordination power to the Lewis acid than the aldehyde compound and having an activity to dissolve the Lewis acid, coordinated by the compound (VIII), in a solvent as a co-catalyst in reacting aldehyde with a diene compound in the presence of a Lewis acid to prepare a 5,6-dihydro-2H-pyrane compound represented by formula (III): in which R represents a hydrogen atom, an alkyl group having 1 to 12 carbon atoms, aryl group which may be substituted with alkyl group, having 6 to 12 total carbon atoms; R and R represent a hydrogen atom or an alkyl group having 1 to 6 carbon atoms.
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公开(公告)号:DE60324127D1
公开(公告)日:2008-11-27
申请号:DE60324127
申请日:2003-04-25
Applicant: KAO CORP
Inventor: HAKAMADA YOSHIHIRO , OZAWA TADAHIRO , KOBAYASHI TOORU
Abstract: The present invention is directed to a mutated alkaline cellulase obtained by deleting, from a cellulase having an amino acid sequence represented by SEQ ID NO: 1 or an amino acid sequence exhibiting at least 90% homology therewith, one or more amino acid residue(s) chosen from the 343rd to 377th positions in SEQ ID NO: 1 or from corresponding positions and inserting a peptide having 2 to 15 amino acid residues into at least one of the deleted positions; and to a gene encoding the mutated alkaline cellulase. The mutated alkaline cellulase has an optimum pH near the pH of the washing liquid (pH: about 10.5) and thus is useful as an enzyme for laundry detergent.
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公开(公告)号:ES2241019T3
公开(公告)日:2005-10-16
申请号:ES97116860
申请日:1997-09-29
Applicant: KAO CORP
Inventor: NISHIOKA TOHRU , TANAKA SHIGEYOSHI , KOSHINO JUNJI , YAMASHITA OSAMU , OZAWA TADAHIRO , KOHAMA MAKOTO
IPC: C07D309/18
Abstract: CON OBJETO DE PROPORCIONAR UN PROCESO SENCILLO PARA LA PREPARACION ECONOMICA DE UN COMPUESTO DE DIHIDROPIRANO CON UN ALTO INDICE DE PRODUCTIVIDAD Y RENDIMIENTO DE REACCION. PARA UTILIZAR UN COMPUESTO SELECCIONADO DEL GRUPO CONSISTENTE EN UNA BASE Y UN COMPUESTO (VIII) CON MENOS PODER DE COORDINACION CON EL ACIDO DE LEWIS QUE EL COMPUESTO ALDEHIDO Y ACTIVIDAD PARA DISOLVER EL ACIDO DE LEWIS, COORDINADA POR EL COMPUESTO (VIII), EN UN DISOLVENTE COMO COCATALIZADOR, HACIENDO REACCIONAR EL ALDEHIDO CON UN COMPUESTO DE DIENO EN PRESENCIA DE UN ACIDO DE LEWIS PARA PREPARAR UN COMPUESTO 5,6 PRESENTADO POR LA FORMULA (III): EN DONDE R SUP,1} REPRESENTA UN ATOMO DE HIDROGENO, UN GRUPO ALQUILO CON 1 A 12 ATOMOS DE CARBONO, UN GRUPO ARILO QUE PUEDE SUSTITUIRSE CON UN GRUPO ALQUILO, CON UN TOTAL DE 6 A 12 ATOMOS DE CARBONO; R SUP,2} Y R SUP,3} REPRESENTAN UN ATOMO DE HIDROGENO O UN GRUPO ALQUILO CON 1 A 6 ATOMOS DE CARBONO.
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公开(公告)号:DE69733524D1
公开(公告)日:2005-07-21
申请号:DE69733524
申请日:1997-09-29
Applicant: KAO CORP
Inventor: NISHIOKA TOHRU , TANAKA SHIGEYOSHI , KOSHINO JUNJI , YAMASHITA OSAMU , OZAWA TADAHIRO , KOHAMA MAKOTO
IPC: C07D309/18
Abstract: To provide a simple process for economically preparing a dihydropyrane compound at a high productivity and a high reaction yield. To use a compound selected from the group consisting of a base and a compound (VIII) having a weaker coordination power to the Lewis acid than the aldehyde compound and having an activity to dissolve the Lewis acid, coordinated by the compound (VIII), in a solvent as a co-catalyst in reacting aldehyde with a diene compound in the presence of a Lewis acid to prepare a 5,6-dihydro-2H-pyrane compound represented by formula (III): in which R represents a hydrogen atom, an alkyl group having 1 to 12 carbon atoms, aryl group which may be substituted with alkyl group, having 6 to 12 total carbon atoms; R and R represent a hydrogen atom or an alkyl group having 1 to 6 carbon atoms.
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公开(公告)号:AU2003235831A1
公开(公告)日:2003-11-10
申请号:AU2003235831
申请日:2003-04-25
Applicant: KAO CORP
Inventor: HAKAMADA YOSHIHIRO , OZAWA TADAHIRO , KOBAYASHI TOORU
Abstract: The present invention is directed to a mutated alkaline cellulase obtained by deleting, from a cellulase having an amino acid sequence represented by SEQ ID NO: 1 or an amino acid sequence exhibiting at least 90% homology therewith, one or more amino acid residue(s) chosen from the 343rd to 377th positions in SEQ ID NO: 1 or from corresponding positions and inserting a peptide having 2 to 15 amino acid residues into at least one of the deleted positions; and to a gene encoding the mutated alkaline cellulase. The mutated alkaline cellulase has an optimum pH near the pH of the washing liquid (pH: about 10.5) and thus is useful as an enzyme for laundry detergent.
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