公开(公告)号：HK104795A

公开(公告)日：1995-07-07

申请号：HK104795

申请日：1995-06-29

Applicant: KAO CORP

Inventor： ITO SUSUMU ,  SHIMOOKA MASAHARU ,  OHTA YUICHI ,  TAKALWA MIKIO ,  ADACHI SHIGEHITO

IPC: C12N9/26 ,  C12N9/42 ,  C12N9/54 ,  C12R1/07

Abstract: A mutant of an extracellular enzyme-producing microorganism belonging to genus Bacillus, which is resistant to a cell membrane synthesis inhibitor, including vancomycin and ristocetin, is disclosed. The cell membrane synthesis inhibitor-resistant mutant can produce such an enzyme as cellulase, protease, or amylase about 2 to 4 times of the parent strain. Such a mutant can be produced by subjecting an extracellular enzyme-producing microorganism to a mutation treatment, and culturing said microorganism in a culture medium containing a cell membrane synthesis inhibitor.

公开(公告)号：DK175284B1

公开(公告)日：2004-08-09

申请号：DK547788

申请日：1988-09-30

Applicant: KAO CORP

Inventor： ITO SUSUMU ,  SHIMOOKA MASAHARU ,  OHTA YUICHI ,  TAKAIWA MIKIO ,  ADACHI SHIGEHITO

IPC: C12N9/26 ,  C12N9/42 ,  C12N9/54 ,  C12N1/20 ,  C12N15/00

Abstract: A mutant of an extracellular enzyme-producing microorganism belonging to genus Bacillus, which is resistant to a cell membrane synthesis inhibitor, including vancomycin and ristocetin, is disclosed. The cell membrane synthesis inhibitor-resistant mutant can produce such an enzyme as cellulase, protease, or amylase about 2 to 4 times of the parent strain. Such a mutant can be produced by subjecting an extracellular enzyme-producing microorganism to a mutation treatment, and culturing said microorganism in a culture medium containing a cell membrane synthesis inhibitor.

公开(公告)号：PH25364A

公开(公告)日：1991-05-13

申请号：PH37638

申请日：1988-10-04

Applicant: KAO CORP

Inventor： ITO SUSUMU ,  SHIMOOKA MASAHARU ,  TAKAIWA MIKIO ,  ADACHI SHIGEHITO ,  OHTA YUICHI

IPC: C12N9/26 ,  C12N9/42 ,  C12N9/54 ,  C12N15/00 ,  C12R1/07

Abstract: A mutant of an extracellular enzyme-producing microorganism belonging to genus Bacillus, which is resistant to a cell membrane synthesis inhibitor, including vancomycin and ristocetin, is disclosed. The cell membrane synthesis inhibitor-resistant mutant can produce such an enzyme as cellulase, protease, or amylase about 2 to 4 times of the parent strain. Such a mutant can be produced by subjecting an extracellular enzyme-producing microorganism to a mutation treatment, and culturing said microorganism in a culture medium containing a cell membrane synthesis inhibitor.

公开(公告)号：DK547788A

公开(公告)日：1989-10-01

申请号：DK547788

申请日：1988-09-30

Applicant: KAO CORP

Inventor： ITO SUSUMU ,  SHIMOOKA MASAHARU ,  OHTA YUICHI ,  TAKAIWA MIKIO ,  ADACHI SHIGEHITO

IPC: C12N9/26 ,  C12N9/42 ,  C12N9/54 ,  C12N1/20 ,  C12N15/00

Abstract: A mutant of an extracellular enzyme-producing microorganism belonging to genus Bacillus, which is resistant to a cell membrane synthesis inhibitor, including vancomycin and ristocetin, is disclosed. The cell membrane synthesis inhibitor-resistant mutant can produce such an enzyme as cellulase, protease, or amylase about 2 to 4 times of the parent strain. Such a mutant can be produced by subjecting an extracellular enzyme-producing microorganism to a mutation treatment, and culturing said microorganism in a culture medium containing a cell membrane synthesis inhibitor.

公开(公告)号：DE3851325T2

公开(公告)日：1995-03-23

申请号：DE3851325

申请日：1988-10-03

Applicant: KAO CORP

Inventor： ITO SUSUMU ,  SHIMOOKA MASAHARU ,  OHTA YUICHI ,  TAKAIWA MIKIO ,  ADACHI SHIGEHITO

IPC: C12N9/26 ,  C12N9/42 ,  C12N9/54 ,  C12R1/07

Abstract: A mutant of an extracellular enzyme-producing microorganism belonging to genus Bacillus, which is resistant to a cell membrane synthesis inhibitor, including vancomycin and ristocetin, is disclosed. The cell membrane synthesis inhibitor-resistant mutant can produce such an enzyme as cellulase, protease, or amylase about 2 to 4 times of the parent strain. Such a mutant can be produced by subjecting an extracellular enzyme-producing microorganism to a mutation treatment, and culturing said microorganism in a culture medium containing a cell membrane synthesis inhibitor.

公开(公告)号：ES2063012T3

公开(公告)日：1995-01-01

申请号：ES88116309

申请日：1988-10-03

Applicant: KAO CORP

Inventor： ITO SUSUMU ,  SHIMOOKA MASAHARU ,  OHTA YUICHI ,  TAKAIWA MIKIO ,  ADACHI SHIGEHITO

IPC: C12N9/26 ,  C12N9/42 ,  C12N9/54 ,  C12R1/07

Abstract: A mutant of an extracellular enzyme-producing microorganism belonging to genus Bacillus, which is resistant to a cell membrane synthesis inhibitor, including vancomycin and ristocetin, is disclosed. The cell membrane synthesis inhibitor-resistant mutant can produce such an enzyme as cellulase, protease, or amylase about 2 to 4 times of the parent strain. Such a mutant can be produced by subjecting an extracellular enzyme-producing microorganism to a mutation treatment, and culturing said microorganism in a culture medium containing a cell membrane synthesis inhibitor.

公开(公告)号：DE3851325D1

公开(公告)日：1994-10-06

申请号：DE3851325

申请日：1988-10-03

Applicant: KAO CORP

Inventor： ITO SUSUMU ,  SHIMOOKA MASAHARU ,  OHTA YUICHI ,  TAKAIWA MIKIO ,  ADACHI SHIGEHITO

IPC: C12N9/26 ,  C12N9/42 ,  C12N9/54 ,  C12R1/07

Abstract: A mutant of an extracellular enzyme-producing microorganism belonging to genus Bacillus, which is resistant to a cell membrane synthesis inhibitor, including vancomycin and ristocetin, is disclosed. The cell membrane synthesis inhibitor-resistant mutant can produce such an enzyme as cellulase, protease, or amylase about 2 to 4 times of the parent strain. Such a mutant can be produced by subjecting an extracellular enzyme-producing microorganism to a mutation treatment, and culturing said microorganism in a culture medium containing a cell membrane synthesis inhibitor.

公开(公告)号：DK547788D0

公开(公告)日：1988-09-30

申请号：DK547788

申请日：1988-09-30

Applicant: KAO CORP

Inventor： ITO SUSUMU ,  SHIMOOKA MASAHARU ,  OHTA YUICHI ,  TAKAIWA MIKIO ,  ADACHI SHIGEHITO

IPC: C12N9/26 ,  C12N9/42 ,  C12N9/54 ,  C12N1/20 ,  C12N15/00

Abstract: A mutant of an extracellular enzyme-producing microorganism belonging to genus Bacillus, which is resistant to a cell membrane synthesis inhibitor, including vancomycin and ristocetin, is disclosed. The cell membrane synthesis inhibitor-resistant mutant can produce such an enzyme as cellulase, protease, or amylase about 2 to 4 times of the parent strain. Such a mutant can be produced by subjecting an extracellular enzyme-producing microorganism to a mutation treatment, and culturing said microorganism in a culture medium containing a cell membrane synthesis inhibitor.

公开(公告)号：JPH01222771A

公开(公告)日：1989-09-06

申请号：JP7784688

申请日：1988-03-30

Applicant: KAO CORP

Inventor： ITO SUSUMU ,  SHIMOOKA MASAHARU ,  OTA YUICHI ,  TAKAIWA MIKIO ,  ADACHI SHIGEHITO

IPC: C12N1/20 ,  C12N9/42 ,  C12R1/07

Abstract: PURPOSE:To produce a mutant resistant to inhibition of cell membrane synthesis in high sufficiency by subjecting a microorganism in Bacillus, producing an extracellular enzyme to mutagenic treatment and culturing the mutant in the medium containing a cell membrane synthesis inhibitor. CONSTITUTION:A microorganism in Bacillus, producing extracellular enzymes (parent strain) is treated with a mutagenic agent, irradiated with ultraviolet rays or radiation to cause mutation. Then, the mutant is cultured in a medium containing a high concentration of vancomycin or ristocetin to select the strain resistant to the inhibition of cell membrane synthesis. The parent strain is, e.g., Bacillus s.p. KSM-655, Bacillus sp. KSM-425. The cell membrane synthesis inhibitor is, e.g., 5-bromouracil or acridine or nitrous acid.