公开(公告)号：DK561687D0

公开(公告)日：1987-10-27

申请号：DK561687

申请日：1987-10-27

Applicant: KAO CORP

Inventor： ITO SUSUMU ,  SATO TOMOKAZU ,  OZAKI KATSUYA ,  SHIKATA SHITSUW ,  OKAMOTO KIKUHIKO ,  INOUE SHIGEO ,  KOIKE KENZO ,  OTA YUICHI ,  TAKEI AKIRA

IPC: C12N9/42 ,  C12N1/20

Abstract: Novel alkaline cellulase K, CMCase I and CMCase II are obtained by isolation from a culture product of Bacillus sp KSM-635. These enzymes stably work in a wide range including an alkaline side, and their activity is shown even at low temperatures. Further, they have a strong resistance to surfce active agents, chelating agents and proteinases. Therefore, they can be effectively utilized not only as an additive for clothing detergents, but also as a biomass and in other fields.

公开(公告)号：SG112094G

公开(公告)日：1994-11-25

申请号：SG112094

申请日：1994-08-12

Applicant: KAO CORP

Inventor： ITO SUSUMU ,  SATO TOMOKAZU ,  OZAKI KATSUYA ,  SHIKATA SHITSUW ,  OKAMOTO KIKUHIKO ,  INOUE SHIGEO ,  KOIKE KENZO ,  OTA YUICHI ,  TAKEI AKIRA

IPC: C12N9/42 ,  C12R1/07

公开(公告)号：DE3787866T2

公开(公告)日：1994-05-19

申请号：DE3787866

申请日：1987-10-21

Applicant: KAO CORP

Inventor： ITO SUSUMU ,  SATO TOMOKAZU ,  OZAKI KATSUYA ,  SHIKATA SHITSUW ,  OKAMOTO KIKUHIKO ,  INOUE SHIGEO ,  KOIKE KENZO ,  OTA YUICHI ,  TAKEI AKIRA

IPC: C12N9/42

Abstract: Novel alkaline cellulase K, CMCase I and CMCase II are obtained by isolation from a culture product of Bacillus sp KSM-635. These enzymes stably work in a wide range including an alkaline side, and their activity is shown even at low temperatures. Further, they have a strong resistance to surfce active agents, chelating agents and proteinases. Therefore, they can be effectively utilized not only as an additive for clothing detergents, but also as a biomass and in other fields.

公开(公告)号：ES2060590T3

公开(公告)日：1994-12-01

申请号：ES87115430

申请日：1987-10-21

Applicant: KAO CORP

Inventor： ITO SUSUMU ,  SATO TOMOKAZU ,  OZAKI KATSUYA ,  SHIKATA SHITSUW ,  OKAMOTO KIKUHIKO ,  INOUE SHIGEO ,  KOIKE KENZO ,  OTA YUICHI ,  TAKEI AKIRA

IPC: C12N9/42

Abstract: Novel alkaline cellulase K, CMCase I and CMCase II are obtained by isolation from a culture product of Bacillus sp KSM-635. These enzymes stably work in a wide range including an alkaline side, and their activity is shown even at low temperatures. Further, they have a strong resistance to surfce active agents, chelating agents and proteinases. Therefore, they can be effectively utilized not only as an additive for clothing detergents, but also as a biomass and in other fields.

公开(公告)号：DE3787866D1

公开(公告)日：1993-11-25

申请号：DE3787866

申请日：1987-10-21

Applicant: KAO CORP

Inventor： ITO SUSUMU ,  SATO TOMOKAZU ,  OZAKI KATSUYA ,  SHIKATA SHITSUW ,  OKAMOTO KIKUHIKO ,  INOUE SHIGEO ,  KOIKE KENZO ,  OTA YUICHI ,  TAKEI AKIRA

IPC: C12N9/42

Abstract: Novel alkaline cellulase K, CMCase I and CMCase II are obtained by isolation from a culture product of Bacillus sp KSM-635. These enzymes stably work in a wide range including an alkaline side, and their activity is shown even at low temperatures. Further, they have a strong resistance to surfce active agents, chelating agents and proteinases. Therefore, they can be effectively utilized not only as an additive for clothing detergents, but also as a biomass and in other fields.

公开(公告)号：PH26059A

公开(公告)日：1992-01-29

申请号：PH37389

申请日：1988-08-10

Applicant: KAO CORP

Inventor： ITO SUSUMO ,  OKAMOTO KIKUHIKO ,  KOIKE KENZO ,  OSAKI KATSUYA ,  OTA YUICHI

IPC: C12N9/42

公开(公告)号：DK561687A

公开(公告)日：1988-04-29

申请号：DK561687

申请日：1987-10-27

Applicant: KAO CORP

Inventor： ITO SUSUMU ,  SATO TOMOKAZU ,  OZAKI KATSUYA ,  SHIKATA SHITSUW ,  OKAMOTO KIKUHIKO ,  INOUE SHIGEO ,  KOIKE KENZO ,  OTA YUICHI ,  TAKEI AKIRA

IPC: C12N9/42 ,  C12N1/20

Abstract: Novel alkaline cellulase K, CMCase I and CMCase II are obtained by isolation from a culture product of Bacillus sp KSM-635. These enzymes stably work in a wide range including an alkaline side, and their activity is shown even at low temperatures. Further, they have a strong resistance to surfce active agents, chelating agents and proteinases. Therefore, they can be effectively utilized not only as an additive for clothing detergents, but also as a biomass and in other fields.

公开(公告)号：JPH01222771A

公开(公告)日：1989-09-06

申请号：JP7784688

申请日：1988-03-30

Applicant: KAO CORP

Inventor： ITO SUSUMU ,  SHIMOOKA MASAHARU ,  OTA YUICHI ,  TAKAIWA MIKIO ,  ADACHI SHIGEHITO

IPC: C12N1/20 ,  C12N9/42 ,  C12R1/07

Abstract: PURPOSE:To produce a mutant resistant to inhibition of cell membrane synthesis in high sufficiency by subjecting a microorganism in Bacillus, producing an extracellular enzyme to mutagenic treatment and culturing the mutant in the medium containing a cell membrane synthesis inhibitor. CONSTITUTION:A microorganism in Bacillus, producing extracellular enzymes (parent strain) is treated with a mutagenic agent, irradiated with ultraviolet rays or radiation to cause mutation. Then, the mutant is cultured in a medium containing a high concentration of vancomycin or ristocetin to select the strain resistant to the inhibition of cell membrane synthesis. The parent strain is, e.g., Bacillus s.p. KSM-655, Bacillus sp. KSM-425. The cell membrane synthesis inhibitor is, e.g., 5-bromouracil or acridine or nitrous acid.

公开(公告)号：JPS63109778A

公开(公告)日：1988-05-14

申请号：JP25777886

申请日：1986-10-28

Applicant: KAO CORP

Inventor： KOIKE KENZO ,  ITO SUSUMU ,  OTA YUICHI ,  OZAKI KATSUYA ,  OKAMOTO KIKUHIKO

IPC: C12N9/42 ,  C12R1/07

Abstract: NEW MATERIAL:An enzyme having the following physical and chemical properties. Action, having CX enzyme activity acting to carboxymethylcellulose (CMC) and weak C1 enzyme activity and beta-glucosidase activity; substrate specificity, acting to CMC, crystalline cellulose, Avicel, cellobiose and p-nitrophenyl cellobioside; working pH, 3-12.5; optimum pH, 6-11.5; working temperature, 5-28 deg.C; optimum temperature, 14-45 deg.C; molecular weight, 170,000+ or -20,000 (by gel chromatography). USE:A chelating agent, a surfactant and a clothing detergent having resistance to protease. PREPARATION:For example, Bacillus sp KSM-635 (FERM P-8872) is cultured in a liquid medium at 34 deg.C for 2 days under aerobic condition, cold ethanol is slowly added to the supernatant liquid of the cultured product to form protein precipitate and the precipitate is dissolved in water, neutralized with dilute acetic acid, dialyzed and freeze-dried to obtain powdery enzyme.

公开(公告)号：JPS63109776A

公开(公告)日：1988-05-14

申请号：JP25777686

申请日：1986-10-28

Applicant: KAO CORP

Inventor： ITO SUSUMU ,  KOIKE KENZO ,  OTA YUICHI ,  TAKEI AKIRA ,  OKAMOTO KIKUHIKO

IPC: C12N9/42 ,  C12R1/07

Abstract: NEW MATERIAL:An alkali cellulase K having the following physical and chemi cal properties. Action, having CX enzyme activity acting to car boxymethylcellulose and weak C1 enzyme activity and beta-glucosidase activity; substrate specificity, acting to carboxymethylcellulose, crystalline cellulose, Avicel, cellobiose and p-nitrophenyl cellobioside; working pH, 4-12; optimum pH, 9-10; working temperature, 10-65 deg.C; optimum temperature, about 40 deg.C; molecular weight, having maximum peak at 180,000+ or -10,000 (by gel chro matography). USE:A surfactant, a chelating agent and a clothing detergent having resistance to protease. PREPARATION:For example, Bacillus sp KSM-635 (FERM P-8872) is cultured in a liquid medium at 34 deg.C for 2 days under aerobic condition, cold ethanol is slowly added to the supernatant liquid of the cultured product to form protein precipitate and the precipitate is dissolved in water, neutralized with acetic acid, dialyzed and freeze-dried.