公开(公告)号：JPH07277955A

公开(公告)日：1995-10-24

申请号：JP6725394

申请日：1994-04-05

Applicant: KAO CORP

Inventor： SOU KEIMEI ,  NAKAMURA MIKIHIKO ,  KUROSAKI TOMIHIRO ,  TSUJII KAORU

IPC: A61K9/127

Abstract: PURPOSE:To obtain a liposome including a reaction product between a phosphoric acid ester and an antibody, exhibiting high target-directing properties and useful as a carrier for a medicine, a diagnostic. CONSTITUTION:This liposome includes a reaction product synthesized by using a phosphoric acid ester represented by the formula [R is a 6 to 36C alkyl or alkenyl; R is a 2 to 3C alkylene; M is H or a cation; (n) is 0 to 6] and an antibody in a ratio of the phosphoric acid ester to the antibody of 50 to 300mol:1mol and culturing the resultant mixture at 0 to 40 deg.C for 1 to 24hr. The liposome is prepared from a mixture containing the reaction product and a membrane-constituting component (e.g. yolk lecithin or dimyristoyl phosphatidylcholine) in an arbitrary ratio according to the conventional method for formation of liposome, e.g. dialysis method. In this liposome, the ratio of the antibody introduced to the liposome surface is high. The activity of the antibody is high since a foreign material such as a coupling agent does not exist on the liposome surface.