公开(公告)号：EP3093665A4

公开(公告)日：2017-09-20

申请号：EP15735557

申请日：2015-01-07

Applicant: KONICA MINOLTA INC

Inventor： NODA TETSUYA

IPC: G01N21/64 ,  G01N33/543

CPC classification number: G01N33/54373 ,  G01N21/648 ,  G01N21/6486 ,  G01N33/543 ,  G01N2021/6417 ,  G01N2201/06113 ,  G01N2201/068

Abstract: Provided are an immunoassay method in which, by using a sensor chip on which a plurality of capture regions which capture a material to be detected by a first capturing body are arranged separated from each other, the material to be detected captured by the first capturing body is individually detected, wherein the plurality of capture regions are formed by using a different type of first capturing body depending on the type of a material to be detected to be captured, the method having: a detection processing order determination step of determining a detection processing order between the capture regions based on information about a detection processing order between the plurality of the capture regions; and a detection processing step of performing a detection processing for each of the capture regions according to the detection processing order between the plurality of the capture regions, and an immunoassay system using the method.

Abstract translation: 本发明提供一种免疫测定方法，其中，通过使用将捕捉第一捕捉体检测对象物质的多个捕捉区域分离的传感器芯片，将由第一捕捉体捕捉的检测对象物质 所述方法具有：检测处理顺序确定步骤，用于确定检测处理的检测处理顺序确定步骤，其中，所述检测处理顺序确定步骤根据待检测材料的类型来检测所述多个捕获区域， 基于关于多个捕获区域之间的检测处理顺序的信息在捕获区域之间的顺序; 以及检测处理步骤，根据多个捕捉区域之间的检测处理顺序，针对每个捕捉区域执行检测处理，以及使用该方法的免疫测定系统。

公开(公告)号：EP3023772A4

公开(公告)日：2017-03-29

申请号：EP14825688

申请日：2014-07-17

Applicant: KONICA MINOLTA INC

Inventor： FUJII HIDEYUKI ,  NODA TETSUYA ,  MIYAZAKI KOJI

IPC: G01N21/64 ,  G01N21/552 ,  G01N33/543

CPC classification number: G01N21/648 ,  G01N21/553 ,  G01N21/6428 ,  G01N33/54373 ,  G01N2021/6439 ,  G01N2021/6463

Abstract: A surface plasmon resonance fluorescence analysis device has a chip holder for holding an analysis chip, a light source for irradiating excitation light, an angle adjustment unit for adjusting the angle of incidence of the excitation light in relation to the interface of a prism and metal film of the analysis chip, an excitation light reflection filter, a first optical sensor for detecting the fluorescence emitted from the analysis chip and transmitted through the excitation light reflection filter, a second optical sensor for detecting the plasmon scattered light emitted from the analysis chip and reflected by the excitation light reflection filter, and a control unit for controlling the angle adjustment unit. The control unit determines an enhancement angle on the basis of the plasmon scattered light detection results of the second optical sensor.

Abstract translation: 的表面等离子体共振荧光分析装置具有用于保持分析用芯片，一个光源用于照射激发光角调整单元，用于调整激发光的入射角度相对于棱镜和金属电影的接口的奇普·霍尔德 分析用芯片的，以激发光反射滤光器，用于检测从分析芯片和反式发射的荧光的第一光学传感器通过所述激发光反射滤光器mitted，第二光学传感器，用于检测从所述的分析芯片发射和反射的等离子体散射光 由激发光反射滤光器，以及用于控制所述角度调整单元的控制单元。 所述控制单元确定的矿山增强第二光学传感器的等离子体散射光的检测结果的基础上的角度。

公开(公告)号：EP3159677A4

公开(公告)日：2018-02-21

申请号：EP15809717

申请日：2015-06-16

Applicant: KONICA MINOLTA INC

Inventor： FUJIYO ICHIRO ,  NODA TETSUYA

IPC: G01N21/64

CPC classification number: G01N21/6428 ,  G01N21/645 ,  G01N21/648 ,  G01N2021/6439 ,  G01N2021/6471 ,  G01N2201/0806

Abstract: A detection device comprises a chip holder, a light source, a light-guide rod, a wavelength separation filter, and an optical sensor. Given the relationship between the angle of incidence and light intensity of fluorescence on a light reception surface of the optical sensor, the optical transmittance of the wavelength separation filter at the dominant wavelength of the rays of fluorescence incident on the light reception surface at a peak angle of incidence at which the light intensity is the highest is greater than the optical transmittance of the wavelength separation filter at the dominant wavelength of the rays of excitation light incident on the light reception surface at the peak angle of incidence and is higher than the optical transmittance of the wavelength separation filter at the dominant wavelength of the rays of fluorescence incident on the light reception surface at an angle of incidence of 0 DEG.

公开(公告)号：EP3153845A4

公开(公告)日：2017-12-06

申请号：EP15799969

申请日：2015-05-29

Applicant: KONICA MINOLTA INC

Inventor： MATSUO MASATAKA ,  HIRAYAMA HIROSHI ,  HAMANO YOSHIMASA ,  NODA TETSUYA ,  OKUMURA YOSHIHIRO

IPC: G01N21/64

CPC classification number: G01N21/648 ,  G01N21/64

Abstract: First, an analytical chip having a prism, a metal film that includes a trapping region having immobilized on the surface thereof a trapping element for trapping a substance to be analyzed, and a mark in which the scatter of emitted plasmon scattered light differs from the scatter of plasmon scattered light emitted from the surrounding region, is disposed in a chip holder. Next, the rear surface of the metal film is irradiated with excitation light, plasmon scattered light emitted from the proximity of the mark is detected, and, on the basis of the detected plasmon scattered light, location information for the trap region is obtained. Next, the portion of the rear surface of the metal film that corresponds to the trap region arranged at the detected location is irradiated with excitation light, and fluorescence emitted by a fluorescent substance is detected.

公开(公告)号：EP3260869A4

公开(公告)日：2018-03-07

申请号：EP16752182

申请日：2016-01-19

Applicant: KONICA MINOLTA INC

Inventor： IWASHITA ATSUO ,  NODA TETSUYA ,  MATSUO MASATAKA ,  MIYAZAKI KOJI ,  SHOUJI YUUYA

IPC: G01N35/10

CPC classification number: G01N35/1011 ,  B01L3/0217 ,  B01L2200/146 ,  B01L2200/148 ,  B01L2400/0487 ,  G01N35/00623 ,  G01N35/10 ,  G01N35/1016

Abstract: In a detection method, a first pressure within a pipette tip is measured when air is sucked into or expelled from the leading end of the pipette tip in a state where the leading end of the pipette tip and a reference part of a solid are separated. A second pressure within the pipette tip is measured when air is sucked into or expelled from the leading end of the pipette tip in a state where the leading end of the pipette tip and the reference part of the solid are closer than in the first step. After the first step and second step, the position of the leading end of the pipette tip in relation to the reference part is detected on the basis of the difference between the first pressure measured in the first step and the second pressure measured in the second step.

公开(公告)号：EP3104168A4

公开(公告)日：2017-10-11

申请号：EP15747011

申请日：2015-02-04

Applicant: KONICA MINOLTA INC

Inventor： FUJII HIDEYUKI ,  NODA TETSUYA ,  NAKAMURA YUKITO

IPC: G01N21/64

CPC classification number: G01N21/648 ,  G01N21/6428 ,  G01N33/54373 ,  G01N2021/6439 ,  G01N2021/6471 ,  G01N2201/0612 ,  G01N2201/0633 ,  G01N2201/068

Abstract: A surface plasmon fluorescence analysis device that has a chip holder, a light source, an angle adjustment unit, a light sensor, a filter holder, an excitation light cut filter, a scattered light transmission unit, a transmission adjustment unit, and a control unit. As seen in plan view, the area occupied by the scattered light transmission unit is arranged on the excitation light cut filter or on the filter holder and is smaller than the area of a fluorescence transmission region as seen in plan view.

公开(公告)号：EP3064929A4

公开(公告)日：2017-06-07

申请号：EP14857305

申请日：2014-10-30

Applicant: KONICA MINOLTA INC

Inventor： NODA TETSUYA ,  MATSUO MASATAKA ,  HAMANO YOSHIMASA ,  HIRAYAMA HIROSHI

IPC: G01N21/64 ,  G01N21/13 ,  G01N21/84

CPC classification number: G01N21/553 ,  G01N21/13 ,  G01N21/648 ,  G01N2021/135 ,  G01N2021/8455

Abstract: In the present invention, excitation light is irradiated onto an analysis chip that has been placed in a chip holder, reflected light or transmitted light from the analysis chip is detected, and as a result the position information of the analysis chip is obtained. On the basis of this position information, the chip holder is moved by a conveyance stage and thereby moved to a measurement position. Excitation light is irradiated onto the analysis chip that is disposed at the measurement position, and fluorescence emitted from a fluorescent substance that marks the substance to be detected is detected.

Abstract translation: 在本发明中，将激发光照射到放置在芯片保持器中的分析芯片上，检测来自分析芯片的反射光或透射光，结果获得分析芯片的位置信息。 基于该位置信息，芯片保持器通过输送台移动并由此移动到测量位置。 将激发光照射到配置在测定位置的分析芯片上，检测从标记检测对象物质的荧光物质发出的荧光。

公开(公告)号：EP3273226A4

公开(公告)日：2018-01-31

申请号：EP16764767

申请日：2016-03-08

Applicant: KONICA MINOLTA INC

Inventor： NODA TETSUYA ,  NAGAI FUMIO ,  YAMAUCHI NOBUHIRO ,  KYOGOKU YUICHI

IPC: G01N21/64

CPC classification number: G01N21/64 ,  G01N21/648 ,  G01N2021/6463 ,  G01N2021/6471 ,  G01N2201/0806

Abstract: This detection device has a holder, light irradiation unit, angle adjustment unit, light receiving sensor, light receiving optical system, optical filter, and a control unit. The light receiving optical system guides light from a detection chip to the light receiving sensor. The optical filter is disposed in the light receiving optical system, blocks a part of plasmon scattered light, and passes, out of the light emitted from the detection chip, a part of the plasmon scattered light, and fluorescence emitted from a fluorescent material. The light receiving sensor detects the fluorescent light, and the part of the plasmon scattered light, which have been emitted from the detection chip and passed the optical filter. On the basis of the detection results of the plasmon scattered light, the control unit controls the angle adjustment unit, and adjusts the incident angle of the excitation light to a predetermined incident angle.

公开(公告)号：JP2015059898A

公开(公告)日：2015-03-30

申请号：JP2013195198

申请日：2013-09-20

Applicant: コニカミノルタ株式会社 ,  Konica Minolta Inc

Inventor： NODA TETSUYA ,  IWASHITA ATSUO ,  TAMURA TSURUTOSHI ,  MIYAZAKI KOJI

IPC: G01N21/64 ,  G01N21/78

Abstract: 【課題】受光センサーの検出レンジを切り替えても、当該切り替えのための超過時間を生じないイムノアッセイ分析方法およびイムノアッセイ分析装置を提供する。【解決手段】SPFS装置において、受光センサーの制御電圧を変更することにより、受光センサーの検出レンジを変更する。シグナル測定前の洗浄プロセスが完了する前(S210、211)に、検出レンジの判定のための事前測定から適切な検出レンジの設定まで(S411〜413)を完了する。本測定の前に検出レンジの最適化が行われ、蛍光標識後の流路の洗浄終了後、即座に本測定が可能となる。【選択図】図3

Abstract translation: 要解决的问题：提供即使当光接收传感器的检测范围被切换时也不会发生切换的剩余时间的免疫测定分析方法和装置。解决方案：通过改变控制来改变光接收传感器的检测范围 在SPFS设备中的光接收传感器的电压。 在信号测量完成之前的清洗处理（S210,211）之前，完成从用于确定检测范围到设置适当检测范围的先前测量的步骤（S411-413）。 在实际测量之前，检测范围得到优化，荧光标签清洗后，立即进行实际测量。

公开(公告)号：JP2017090336A

公开(公告)日：2017-05-25

申请号：JP2015223060

申请日：2015-11-13

Applicant: コニカミノルタ株式会社 ,  Konica Minolta Inc

Inventor： NODA TETSUYA ,  KUNIMOTO AKIRA ,  YAMAUCHI NOBUHIRO ,  KYOGOKU YUICHI

IPC: G01N21/64 ,  G01N33/533

Abstract: 【課題】ゲイン判定のための励起光の照射による蛍光物質の褪色を抑制することができる測定方法を提供すること。【解決手段】まず、測定チップに第1の光量で励起光を照射するとともに、蛍光物質から放出された蛍光を検出し、検出された蛍光の光量を示す蛍光信号を、ゲインアンプにより複数のゲインでそれぞれ増幅して、複数の増幅値を得る。次いで、蛍光信号を増幅するためのゲインの大きさを設定する。次いで、第1の光量よりも多い第2の光量で、測定チップに励起光を照射するとともに、蛍光物質から放出された蛍光を検出し、検出された蛍光の光量を示す蛍光信号を、ゲインアンプにより、設定された大きさのゲインで増幅して、増幅値を得る。ゲインの大きさは、第1の光量および第2の光量の光量差と、複数の増幅値とに基づいて設定される。【選択図】図7