公开(公告)号：US20220235106A1

公开(公告)日：2022-07-28

申请号：US17601125

申请日：2020-04-01

Applicant: KOREA INSTITUTE OF CERAMIC ENGINEERING AND TECHNOLOGY

Inventor： Sung Hyun KIM ,  Jin Ho BANG ,  Mi Hyun CHOI

IPC: C07K14/47 ,  C12N9/64 ,  C12N9/74 ,  C07K14/78 ,  C07K14/485 ,  C07K14/50 ,  C07K14/49 ,  C07K14/51 ,  C07K14/495 ,  C07K14/605 ,  C12N9/08

Abstract: The present invention relates to a method for expressing, water-solubilizing, and purifying protein by using a calsequestrin-tag (CSQ-tag). Provided are: a recombinant expression vector containing a CSQ-tag and a target protein; a host cell transformed using the recombinant expression vector; and a method for expressing and purifying a target protein by using a CSQ tag. The present invention uses CSQ-tags to increase the expression of proteins that are widely used in pharmaceuticals and cosmetics, and allows the proteins to be easily separated by calcium, and thus is expected to be able to lower the cost of protein materials and protein pharmaceuticals.