公开(公告)号：US20220235106A1

公开(公告)日：2022-07-28

申请号：US17601125

申请日：2020-04-01

Applicant: KOREA INSTITUTE OF CERAMIC ENGINEERING AND TECHNOLOGY

Inventor： Sung Hyun KIM ,  Jin Ho BANG ,  Mi Hyun CHOI

IPC: C07K14/47 ,  C12N9/64 ,  C12N9/74 ,  C07K14/78 ,  C07K14/485 ,  C07K14/50 ,  C07K14/49 ,  C07K14/51 ,  C07K14/495 ,  C07K14/605 ,  C12N9/08

Abstract: The present invention relates to a method for expressing, water-solubilizing, and purifying protein by using a calsequestrin-tag (CSQ-tag). Provided are: a recombinant expression vector containing a CSQ-tag and a target protein; a host cell transformed using the recombinant expression vector; and a method for expressing and purifying a target protein by using a CSQ tag. The present invention uses CSQ-tags to increase the expression of proteins that are widely used in pharmaceuticals and cosmetics, and allows the proteins to be easily separated by calcium, and thus is expected to be able to lower the cost of protein materials and protein pharmaceuticals.

公开(公告)号：US20230043898A1

公开(公告)日：2023-02-09

申请号：US17936657

申请日：2022-09-29

Applicant: KOREA INSTITUTE OF CERAMIC ENGINEERING AND TECHNOLOGY

Inventor： Sung Hyun KIM ,  Hee Sun PARK

IPC: C12N15/62 ,  C07K1/22 ,  C07K14/47 ,  C07K16/00 ,  C07K19/00 ,  C12N15/70

Abstract: The present disclosure relates to a fusion protein of Z-domain and calsequestrin having improved reactivity, stability, and antibody recovery, and a method of isolating and purifying antibodies using the same. Specifically, the present disclosure relates to: a nucleic acid encoding a fusion protein of Z-domain and calsequestrin having improved reactivity, stability, and antibody recovery; a recombinant expression vector including the nucleic acid; a host cell transformed with the recombinant expression vector; and a method of isolating and purifying antibodies by using the fusion protein of Z-domain and calsequestrin having improved reactivity, stability, antibody recovery, and purity.