公开(公告)号：US09598717B2

公开(公告)日：2017-03-21

申请号：US14345835

申请日：2012-09-18

Applicant: KOREA RESEARCH INSTITUTE OF STANDARDS AND SCIENCE

Inventor： Duk Jin Kang ,  Sang Ryoul Park ,  Sook-Kyung Kim

IPC: C12Q1/37 ,  C07K1/34 ,  C12M1/40 ,  C12M1/12 ,  C12P21/06 ,  C07K1/36 ,  C07K1/20

CPC classification number: C12Q1/37 ,  C07K1/20 ,  C07K1/34 ,  C07K1/36 ,  C12M21/18 ,  C12M25/12 ,  C12P21/06

Abstract: Provided are an enzyme treatment apparatus for proteins using a hollow fiber membrane and an on-line proteomics method using same. The enzyme treatment apparatus for proteins according to the present invention can increase a recovery rate of peptides, which are recovered through an enzyme treatment process, by basically solving the problem of low reproducibility of an enzyme activity which may occur during a conventional enzyme treatment process, and can also reduce a time for purification and provide higher yield by performing separation and purification through a single step. In particular, the present invention can be usefully applied for developing a disease-specific protein biomarker through a statistical analysis method having highly efficient detectability for proteins in research for finding biomarkers related to human diseases.

公开(公告)号：US20140302545A1

公开(公告)日：2014-10-09

申请号：US14345835

申请日：2012-09-18

Applicant: KOREA RESEARCH INSTITUTE OF STANDARDS AND SCIENCE

Inventor： Duk Jin Kang ,  Sang Ryoul Park ,  Sook-Kyung Kim

IPC: C12Q1/37

CPC classification number: C12Q1/37 ,  C07K1/20 ,  C07K1/34 ,  C07K1/36 ,  C12M21/18 ,  C12M25/12 ,  C12P21/06

Abstract: Provided are an enzyme treatment apparatus for proteins using a hollow fiber membrane and an on-line proteomics method using same. The enzyme treatment apparatus for proteins according to the present invention can increase a recovery rate of peptides, which are recovered through an enzyme treatment process, by basically solving the problem of low reproducibility of an enzyme activity which may occur during a conventional enzyme treatment process, and can also reduce a time for purification and provide higher yield by performing separation and purification through a single step. In particular, the present invention can be usefully applied for developing a disease-specific protein biomarker through a statistical analysis method having highly efficient detectability for proteins in research for finding biomarkers related to human diseases.

Abstract translation: 提供了使用中空纤维膜的蛋白质的酶处理装置和使用其的在线蛋白质组学方法。 根据本发明的蛋白质的酶处理装置可以通过基本上解决在常规酶处理过程中可能发生的酶活性的低再现性的问题来提高通过酶处理过程回收的肽的回收率， 并且还可以通过单步执行分离和纯化来减少纯化时间并提供更高的产率。 特别地，本发明可以有用地用于通过统计分析方法开发疾病特异性蛋白质生物标志物，所述统计分析方法在用于寻找与人类疾病相关的生物标志物的研究中具有高度可检测性的蛋白质。