公开(公告)号：US20150210746A1

公开(公告)日：2015-07-30

申请号：US14657228

申请日：2015-03-13

Applicant: KOREA RESEARCH INSTITUTE OF STANDARDS AND SCIENCE

Inventor： Jun-Hyuk Choi ,  Sook-Kyung Kim ,  Minji Kim

IPC: C07K14/61 ,  B01D15/42 ,  B01D15/34 ,  B01D15/38 ,  B01D15/36

CPC classification number: C07K14/61 ,  B01D15/34 ,  B01D15/363 ,  B01D15/3823 ,  B01D15/426

Abstract: The present invention relates to a method for expressing, extracting and refining recombinant human growth hormone (hGH). More particularly, the present invention relates to a method for the intracellular expression of a target protein with minimizing the formation of insoluble inclusion bodies during the mass production of the protein in Escherichia coli system. The present invention also relates to a method for extracting and refining the target protein, particularly human growth hormone, with maximizing its solubility during the extraction and with providing a high yield but without losing the biological activity of the target protein.

Abstract translation: 本发明涉及用于表达，提取和精制重组人生长激素（hGH）的方法。 更具体地说，本发明涉及一种在大肠杆菌系统中大量生产蛋白质期间最小化不溶性包涵体形成的靶蛋白的细胞内表达的方法。 本发明还涉及一种用于提取和精制目标蛋白质，特别是人生长激素的方法，其在提取期间最大限度地提高其溶解度并提供高产率但不丧失靶蛋白的生物活性。