公开(公告)号：US20170044629A1

公开(公告)日：2017-02-16

申请号：US15336499

申请日：2016-10-27

Applicant: KOREA RESEARCH INSTITUTE OF STANDARDS AND SCIENCE

Inventor： Hyong Ha Kim ,  Woo Jeong Kim ,  Jung Keun Suh

IPC: C12Q1/68

CPC classification number: C12Q1/6895 ,  C12Q2600/13 ,  C12Q2600/166 ,  G01N33/5097

Abstract: A method of manufacturing a reference material for determining incorporation of a genetically modified (GM) plant into a sample or analyzing a mixing ratio from a tissue-cultured cell line that is obtained by incubating tissues of either a GM plant or a non-GM plant, and a method of determining incorporation of a GM plant into a sample and analyzing a mixing ratio using the reference material are provided. The reference material for determining the incorporation of a genetically modified (GM) plant a sample or analyzing a mixing ratio using the tissue-cultured cell lines that are obtained by incubating tissues of the GM plant and the non-GM plant can be useful in producing a countless number of populations having the same genetic traits via the tissue culture. Thus, when a culture capacity of the reference material is increased to a large volume, it is possible to obtain a large volume of the reference material having uniform qualities with no quality variation between batches. Unlike the conventional reference materials manufactured using grain powder, a reference material with 100% purity can be obtained as either a GM or non-GM reference material by verifying the purity of the tissue-cultured cell line. Accordingly, it is possible to provide the uniform and stable supply of a reference material having uniform compositions.

Abstract translation: 制造用于确定将遗传修饰（GM）植物掺入样品或参照通过将转基因植物或非转基因植物的组织培养而获得的组织培养细胞系的混合比率进行分析的参考物质的方法 并且提供了确定GM植物掺入样品并使用参考物质分析混合比的方法。 用于确定转基因植物（GM）植物掺入样品或使用通过温育转基因植物和非转基因植物的组织获得的组织培养的细胞系分析混合比的参考材料可用于生产 通过组织培养具有相同遗传性状的无数个种群。 因此，当参考材料的培养能力增加到大体积时，可以获得大量的具有均匀品质的参考材料，而在批次之间没有质量变化。 与使用谷物粉末制造的常规参考材料不同，通过验证组织培养的细胞系的纯度，可以获得具有100％纯度的参考材料作为GM或非GM参考材料。 因此，可以提供具有均匀组成的参考材料的均匀稳定的供给。

公开(公告)号：US09562267B2

公开(公告)日：2017-02-07

申请号：US14942241

申请日：2015-11-16

Applicant: KOREA RESEARCH INSTITUTE OF STANDARDS AND SCIENCE

Inventor： Hyong Ha Kim ,  Woo Jeong Kim ,  Su Kyung Lee ,  Jung Keun Suh ,  Young Hye Seo

IPC: C12Q1/68 ,  C12N15/82 ,  C07K14/42 ,  C12N9/10

CPC classification number: C12Q1/6876 ,  C07K14/42 ,  C12N9/1092 ,  C12N15/8275 ,  C12Q1/6895 ,  C12Q2600/13 ,  C12Q2600/158 ,  C12Q2600/166 ,  C12Y205/01019

Abstract: Provided is a standard plasmid for assaying a genetically modified plant, a method for quantitatively analyzing a target transgene within the genetically modified plant using the standard plasmid, and a kit for quantitatively analyzing the genetically modified plant including the standard plasmid, wherein the standard plasmid of the present invention is possible to be utilized for assaying a genome containing a 5-enoyl-4-pyruvylshikimate-3-phosphate synthase (EPSPS) gene or a cry1Ab gene, and in particular, significantly useful as a standard material capable of analyzing whether the genetically modified plant such as soybean RRS or GM maize MON810 is incorporated or an incorporation ratio thereof.

Abstract translation: 提供了用于测定遗传修饰植物的标准质粒，使用标准质粒定量分析遗传修饰植物内的靶转基因的方法，以及用于定量分析包含标准质粒的遗传修饰植物的试剂盒，其中标准质粒 本发明可用于测定含有5-烯酰基-4-丙酮酸莽草酸-3-莽草酸-3-磷酸合成酶（EPSPS）基因或cry1Ab基因的基因组，特别是作为能够分析 转基因植物如大豆RRS或GM玉米MON810被掺入或其结合比率。