公开(公告)号：US09562267B2

公开(公告)日：2017-02-07

申请号：US14942241

申请日：2015-11-16

Applicant: KOREA RESEARCH INSTITUTE OF STANDARDS AND SCIENCE

Inventor： Hyong Ha Kim ,  Woo Jeong Kim ,  Su Kyung Lee ,  Jung Keun Suh ,  Young Hye Seo

IPC: C12Q1/68 ,  C12N15/82 ,  C07K14/42 ,  C12N9/10

CPC classification number: C12Q1/6876 ,  C07K14/42 ,  C12N9/1092 ,  C12N15/8275 ,  C12Q1/6895 ,  C12Q2600/13 ,  C12Q2600/158 ,  C12Q2600/166 ,  C12Y205/01019

Abstract: Provided is a standard plasmid for assaying a genetically modified plant, a method for quantitatively analyzing a target transgene within the genetically modified plant using the standard plasmid, and a kit for quantitatively analyzing the genetically modified plant including the standard plasmid, wherein the standard plasmid of the present invention is possible to be utilized for assaying a genome containing a 5-enoyl-4-pyruvylshikimate-3-phosphate synthase (EPSPS) gene or a cry1Ab gene, and in particular, significantly useful as a standard material capable of analyzing whether the genetically modified plant such as soybean RRS or GM maize MON810 is incorporated or an incorporation ratio thereof.

Abstract translation: 提供了用于测定遗传修饰植物的标准质粒，使用标准质粒定量分析遗传修饰植物内的靶转基因的方法，以及用于定量分析包含标准质粒的遗传修饰植物的试剂盒，其中标准质粒 本发明可用于测定含有5-烯酰基-4-丙酮酸莽草酸-3-莽草酸-3-磷酸合成酶（EPSPS）基因或cry1Ab基因的基因组，特别是作为能够分析 转基因植物如大豆RRS或GM玉米MON810被掺入或其结合比率。