公开(公告)号：US10191046B2

公开(公告)日：2019-01-29

申请号：US15603212

申请日：2017-05-23

Applicant: Se-Hwan Paek ,  KOREA UNIVERSITY RESEARCH AND BUSINESS FOUNDATION

Inventor： Se-Hwan Paek ,  Ji-Na Park ,  Sung-Ho Paek

IPC: G01N33/558 ,  B01L3/00 ,  G01N21/78 ,  G01N21/84 ,  G01N21/77

Abstract: An immunobiosensor is disclosed. The immunobiosensor is based on a membrane lateral flow immuno-chromatographic assay (LF-ICA). The immunobiosensor includes: a metal binding protein 10 whose conformation changes upon reaction with a metal ion 1 in a sample; a sensing antibody 20 reacting with the conformationally changed metal binding protein 10 as an antigen; a signal substance 30 conjugated with the metal binding protein 10 or the sensing antibody 20 to form a signal conjugate 30a or 30b; a signal generator 40 reacting with the signal conjugate 30a or 30b to generate a reaction signal; and a reaction strip 50 in the form of a porous membrane adapted to move the sample and where the antigen-antibody reaction occurs and the reaction signal is generated. Also disclosed is a sensor system including the immunobiosensor.

公开(公告)号：US10799861B2

公开(公告)日：2020-10-13

申请号：US15820719

申请日：2017-11-22

Applicant: KOREA UNIVERSITY RESEARCH AND BUSINESS FOUNDATION, SEJONG CAMPUS

Inventor： Se-Hwan Paek

IPC: B01L3/00 ,  G01N33/558 ,  G01N33/68 ,  G01N33/74 ,  H04N9/07 ,  H04N5/225 ,  G01N33/543 ,  C12Q1/26

Abstract: Disclosed is a biochemical-immunological hybrid biosensor. The biochemical-immunological hybrid biosensor includes a reaction strip in the form of a porous membrane through which a sample moves by capillary action. The reaction strip can simultaneously measure heterogeneous multiple biomarkers through both a biochemical analysis and an immunoassay in an independent manner based on membrane chromatography to diagnose a particular disease.

公开(公告)号：US10889794B2

公开(公告)日：2021-01-12

申请号：US14385829

申请日：2012-12-27

Applicant: KOREA UNIVERSITY RESEARCH AND BUSINESS FOUNDATION, SEJONG CAMPUS

Inventor： Sung-Kyu Seo ,  Geon-Soo Jin ,  Un-Hwan Ha ,  Se-Hwan Paek ,  Seung-Pil Pack

IPC: G01N21/01 ,  G01N33/487 ,  G01J1/02 ,  C12M1/34

Abstract: The present invention relates to an apparatus that uses shadow images of cells to continuously measure cell activity at a high processing rate in order to provide cell activity and cell number results. According to one embodiment of the present invention, instead of a highly experienced examiner or technician using a microscope, ELISA reader, etc. having to collect various cell activity measurements and cell numbers, the collection of said information can be automated so as to reduce cost and largely reduce errors in measurements through the development of computer software coupled with hardware using low cost and compact optoelectronic components and simple image processing techniques.

公开(公告)号：US09983202B1

公开(公告)日：2018-05-29

申请号：US15412239

申请日：2017-01-23

Applicant: Korea University Research and Business Foundation

Inventor： Se-Hwan Paek ,  Sung-Min Seo

IPC: G01N33/533

CPC classification number: G01N33/533

Abstract: Disclosed is a method of synthesizing a polymeric fluorescent tracer for signal amplification, including (a) subjecting a streptavidin-fluorophore conjugate (SA-FL) and a spacer-attached biotin-conjugated antibody (b-Ab) to reaction at least two times; (b) treating a dual biotin oligomer spacer (db-oN); and (c) performing a homogenization process.

公开(公告)号：US11154857B2

公开(公告)日：2021-10-26

申请号：US16389180

申请日：2019-04-19

Applicant: KOREA UNIVERSITY RESEARCH AND BUSINESS FOUNDATION, SEJONG CAMPUS

Inventor： Se-Hwan Paek

IPC: G01N21/78 ,  G01N33/52 ,  B01L3/00 ,  G01N33/543 ,  G01N21/76

Abstract: A single-channel, chemical-immunological hybrid biosensor is disclosed. According to an embodiment, the hybrid biosensor includes a reaction strip 100 in the form of a porous membrane through which a sample 1 moves by capillary action. the reaction strip 100 is in the form of a porous membrane through which the sample 1 moves by capillary action. Biomarkers in the sample 1 are independently detected based on a chemical reaction and binding reactions on the reaction strip 100.

公开(公告)号：US20170336405A1

公开(公告)日：2017-11-23

申请号：US15603212

申请日：2017-05-23

Applicant: Se-Hwan Paek ,  KOREA UNIVERSITY RESEARCH AND BUSINESS FOUNDATION

Inventor： Se-Hwan Paek ,  Ji-Na Park ,  Sung-Ho Paek

IPC: G01N33/558 ,  G01N21/78 ,  B01L3/00 ,  G01N33/543

CPC classification number: G01N33/558 ,  B01L3/5023 ,  B01L2300/0654 ,  B01L2300/0825 ,  G01N21/78 ,  G01N21/8483 ,  G01N2021/7759 ,  G01N2021/7786 ,  G01N2333/4727

Abstract: An immunobiosensor is disclosed. The immunobiosensor is based on a membrane lateral flow immuno-chromatographic assay (LF-ICA). The immunobiosensor includes: a metal binding protein 10 whose conformation changes upon reaction with a metal ion 1 in a sample; a sensing antibody 20 reacting with the conformationally changed metal binding protein 10 as an antigen; a signal substance 30 conjugated with the metal binding protein 10 or the sensing antibody 20 to form a signal conjugate 30a or 30b; a signal generator 40 reacting with the signal conjugate 30a or 30b to generate a reaction signal; and a reaction strip 50 in the form of a porous membrane adapted to move the sample and where the antigen-antibody reaction occurs and the reaction signal is generated. Also disclosed is a sensor system including the immunobiosensor.

公开(公告)号：US20150050684A1

公开(公告)日：2015-02-19

申请号：US14385829

申请日：2012-12-27

Applicant: KOREA UNIVERSITY RESEARCH AND BUSINESS FOUNDATION

Inventor： Sung-Kyu Seo ,  Geon-Soo Jin ,  Un-Hwan Ha ,  Se-Hwan Paek ,  Seung-Pil Pack

IPC: G01N33/487 ,  G01J1/02 ,  G01N21/01

CPC classification number: C12M41/46 ,  G01J1/0295

Abstract: The present invention relates to an apparatus that uses shadow images of cells to continuously measure cell activity at a high processing rate in order to provide cell activity and cell number results. According to one embodiment of the present invention, instead of a highly experienced examiner or technician using a microscope, ELISA reader, etc. having to collect various cell activity measurements and cell numbers, the collection of said information can be automated so as to reduce cost and largely reduce errors in measurements through the development of computer software coupled with hardware using low cost and compact optoelectronic components and simple image processing techniques.

Abstract translation: 本发明涉及使用细胞的阴影图像以高处理速率连续测量细胞活性以便提供细胞活性和细胞数目结果的装置。 根据本发明的一个实施方案，代替使用显微镜，ELISA读取器等的经验丰富的检验者或技术人员必须收集各种细胞活性测量值和细胞数量，可以自动地收集所述信息以降低成本 并通过开发与硬件相结合的计算机软件，通过低成本，紧凑的光电子元件和简单的图像处理技术，大大减少了测量误差。