公开(公告)号：EP2799551A1

公开(公告)日：2014-11-05

申请号：EP12860693.6

申请日：2012-12-20

Applicant: Korea Basic Science Institute

Inventor： LEE, Zee-Won ,  KIM, Soohyun ,  KIM, Seung II ,  HWANG, Jung Me

IPC: C12N15/63 ,  C12N9/12 ,  C12N5/10 ,  G01N33/52

CPC classification number: G01N33/5035 ,  C12N15/1055 ,  G01N33/5008 ,  G01N33/5011 ,  G01N33/581 ,  G01N33/582 ,  G01N2333/90666 ,  G01N2333/912 ,  G01N2500/02 ,  G01N2500/10

Abstract: The present invention relates to a method for detecting the interactions of biomaterials, and more specifically, to a method for screening a prey which interacts with a bait, the method including: (a) preparing a cell which expresses (i) a first construct comprising a translocation module, a first labeling material, and a first medium, and (ii) a third construct comprising a prey and a second labeling material; (b) introducing (iii) a second construct into the prepared cell, the second construct comprising a bait and a second medium binding with the first medium; (c) allowing the prey and the bait to interact each other; and (d) confirming the interaction between the prey and the bait by detecting intracellular distributions of the first construct and the third construct. Further, the present invention relates to a cell expressing the first construct and the third construct. Still further, the present invention relates to a screening kit comprising the cell and the second construct. The methods of the present invention can overcome disadvantages including inaccuracy and complexity of the existing techniques for biomaterial interaction detection, and can measure the interaction between a material which is not directly produced through gene translation and gene expression in the cell and a material expressed in the cell, and thus are effective in detecting interactions of a broad range of biomaterials.

公开(公告)号：EP2799551B1

公开(公告)日：2018-07-04

申请号：EP12860693.6

申请日：2012-12-20

Applicant: Korea Basic Science Institute

Inventor： LEE, Zee-Won ,  KIM, Soohyun ,  KIM, Seung II ,  HWANG, Jung Me ,  LEE, Kyung-Bok

IPC: C12N15/10 ,  G01N33/50 ,  G01N33/58

CPC classification number: G01N33/5035 ,  C12N15/1055 ,  G01N33/5008 ,  G01N33/5011 ,  G01N33/581 ,  G01N33/582 ,  G01N2333/90666 ,  G01N2333/912 ,  G01N2500/02 ,  G01N2500/10

Abstract: The present invention relates to a method for detecting the interactions of biomaterials, and more specifically, to a method for screening a prey which interacts with a bait, the method including: (a) preparing a cell which expresses (i) a first construct comprising a translocation module, a first labeling material, and a first medium, and (ii) a third construct comprising a prey and a second labeling material; (b) introducing (iii) a second construct into the prepared cell, the second construct comprising a bait and a second medium binding with the first medium; (c) allowing the prey and the bait to interact each other; and (d) confirming the interaction between the prey and the bait by detecting intracellular distributions of the first construct and the third construct. Further, the present invention relates to a cell expressing the first construct and the third construct. Still further, the present invention relates to a screening kit comprising the cell and the second construct. The methods of the present invention can overcome disadvantages including inaccuracy and complexity of the existing techniques for biomaterial interaction detection, and can measure the interaction between a material which is not directly produced through gene translation and gene expression in the cell and a material expressed in the cell, and thus are effective in detecting interactions of a broad range of biomaterials.