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Patent Agency Ranking
公开(公告)号：WO2005016948A1
公开(公告)日：2005-02-24
申请号：PCT/KR2003/001654
申请日：2003-08-14
Applicant: KOREA BASIC SCIENCE INSTITUTE , AHN, Yeong Hee , YOO, Jong Shin , KIM, Soohyun
Inventor： AHN, Yeong Hee , YOO, Jong Shin , KIM, Soohyun
IPC: C07H1/00
CPC classification number: C07H1/00
Abstract: The present invention relates to the method for tagging of carbohydrates with active methylene compound. Particularly, it relates to the method for tagging of carbohydrates with active methylene compound comprising the step of preparing carbohydrate conjugate in which carbohydrate and methylene compound are combined by mixing carbohydrate mixture and methylene compound under aqueous polar aprotic solvent containing amine base catalyst. The tagging method of the present invention does not need many kinds of chemical reagent and the reactions can be taken even in the presence of certain amount of impurities. So, it can be used for the analysis of oligosaccharide present in the various kinds of samples.
Abstract translation: 本发明涉及用活性亚甲基化合物标记碳水化合物的方法。 特别涉及用活性亚甲基化合物标记碳水化合物的方法，包括制备碳水化合物缀合物的步骤，其中碳水化合物和亚甲基化合物通过混合碳水化合物混合物和亚甲基化合物在含有胺基催化剂的极性非质子溶剂的水溶液下混合。 本发明的标记方法不需要多种化学试剂，即使在存在一定量的杂质的情况下也可以进行反应。 因此，可用于各种样品中存在的寡糖的分析。
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公开(公告)号：EP2799551B1
公开(公告)日：2018-07-04
申请号：EP12860693.6
申请日：2012-12-20
Applicant: Korea Basic Science Institute
Inventor： LEE, Zee-Won , KIM, Soohyun , KIM, Seung II , HWANG, Jung Me , LEE, Kyung-Bok
CPC classification number: G01N33/5035 , C12N15/1055 , G01N33/5008 , G01N33/5011 , G01N33/581 , G01N33/582 , G01N2333/90666 , G01N2333/912 , G01N2500/02 , G01N2500/10
Abstract: The present invention relates to a method for detecting the interactions of biomaterials, and more specifically, to a method for screening a prey which interacts with a bait, the method including: (a) preparing a cell which expresses (i) a first construct comprising a translocation module, a first labeling material, and a first medium, and (ii) a third construct comprising a prey and a second labeling material; (b) introducing (iii) a second construct into the prepared cell, the second construct comprising a bait and a second medium binding with the first medium; (c) allowing the prey and the bait to interact each other; and (d) confirming the interaction between the prey and the bait by detecting intracellular distributions of the first construct and the third construct. Further, the present invention relates to a cell expressing the first construct and the third construct. Still further, the present invention relates to a screening kit comprising the cell and the second construct. The methods of the present invention can overcome disadvantages including inaccuracy and complexity of the existing techniques for biomaterial interaction detection, and can measure the interaction between a material which is not directly produced through gene translation and gene expression in the cell and a material expressed in the cell, and thus are effective in detecting interactions of a broad range of biomaterials.
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3.发明公开
公开(公告)号：EP1654267A1
公开(公告)日：2006-05-10
申请号：EP03818122.8
申请日：2003-08-14
Applicant: Korea Basic Science Institute
Inventor： AHN, Yeong Hee , YOO, Jong Shin , KIM, Soohyun
IPC: C07H1/00
CPC classification number: C07H1/00
Abstract: The present invention relates to the method for tagging of carbohydrates with active methylene compound. Particularly, it relates to the method for tagging of carbohydrates with active methylene compound comprising the step of preparing carbohydrate conjugate in which carbohydrate and methylene compound are combined by mixing carbohydrate mixture and methylene compound under aqueous polar aprotic solvent containing amine base catalyst. The tagging method of the present invention does not need many kinds of chemical reagent and the reactions can be taken even in the presence of certain amount of impurities. So, it can be used for the analysis of oligosaccharide present in the various kinds of samples.
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公开(公告)号：EP2799551A1
公开(公告)日：2014-11-05
申请号：EP12860693.6
申请日：2012-12-20
Applicant: Korea Basic Science Institute
Inventor： LEE, Zee-Won , KIM, Soohyun , KIM, Seung II , HWANG, Jung Me
CPC classification number: G01N33/5035 , C12N15/1055 , G01N33/5008 , G01N33/5011 , G01N33/581 , G01N33/582 , G01N2333/90666 , G01N2333/912 , G01N2500/02 , G01N2500/10
Abstract: The present invention relates to a method for detecting the interactions of biomaterials, and more specifically, to a method for screening a prey which interacts with a bait, the method including: (a) preparing a cell which expresses (i) a first construct comprising a translocation module, a first labeling material, and a first medium, and (ii) a third construct comprising a prey and a second labeling material; (b) introducing (iii) a second construct into the prepared cell, the second construct comprising a bait and a second medium binding with the first medium; (c) allowing the prey and the bait to interact each other; and (d) confirming the interaction between the prey and the bait by detecting intracellular distributions of the first construct and the third construct. Further, the present invention relates to a cell expressing the first construct and the third construct. Still further, the present invention relates to a screening kit comprising the cell and the second construct. The methods of the present invention can overcome disadvantages including inaccuracy and complexity of the existing techniques for biomaterial interaction detection, and can measure the interaction between a material which is not directly produced through gene translation and gene expression in the cell and a material expressed in the cell, and thus are effective in detecting interactions of a broad range of biomaterials.
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5.发明授权
公开(公告)号：EP1654267B1
公开(公告)日：2010-04-21
申请号：EP03818122.8
申请日：2003-08-14
Applicant: Korea Basic Science Institute
Inventor： AHN, Yeong Hee , YOO, Jong Shin , KIM, Soohyun
IPC: C07H1/00
CPC classification number: C07H1/00
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