公开(公告)号：EP1272529A1

公开(公告)日：2003-01-08

申请号：EP00981902.0

申请日：2000-11-30

Applicant: Korea Institute of Science and Technology

Inventor： JEONG, Seo, Young ,  BAE, Eunhee ,  KWON, Ick Chan ,  CHOI, Kuiwon

IPC: C08B37/08 ,  C12N11/16

CPC classification number: C08J9/28 ,  C08B37/003 ,  C08J2201/0484 ,  C08J2305/08 ,  C08L5/08 ,  C12N5/0075 ,  C12N2533/72

Abstract: The present invention relates to macroporous chitosan beads having 5-200 νm in size of relatively large and uniform pores that are distributed from surface to core region, and a preparation method thereof comprising the following steps: by dropping chitosan solution, aqueous chitosan solution or their mixture into the low-temperature of organic solvent or liquid nitrogen; and by regulating pore size by phase separation method via temperature difference. The macroporous chitosan beads of the present invention make cell culture more efficient than the previous substrate, since cell can attach to them efficiently due to their large surface area, it is easy for cell to be injected into them and cell attached to them can exist longer due to their three-dimensional structure, therefore they can be used for a study about production of protein, antibiotics, anticancer agent, polysaccharide, physiologically active agent, animal hormone, or plant hormone as well as a study about substitution of metabolic organs, cartilage or bone.

Abstract translation: 本发明涉及大孔径壳聚糖珠粒及其制备方法，所述大孔径壳聚糖粒子的粒径为5〜200ν，具有较大且均匀的孔隙，分布于表面至核心区域，包括以下步骤：将壳聚糖溶液，壳聚糖水溶液或其 混合物转化为低温的有机溶剂或液氮; 并通过温度差通过相分离法调节孔径。 本发明的大孔脱乙酰壳多糖珠粒使细胞培养比先前的基质更有效，因为细胞由于它们的大表面积而可以有效地附着到它们上，细胞很容易注入它们并且细胞附着在它们上的时间可以更长 由于它们的三维结构，因此它们可以用于研究蛋白质，抗生素，抗癌剂，多糖，生理活性剂，动物激素或植物激素的生产以及代谢器官，软骨替代的研究 或骨头。