公开(公告)号：DE102008058068A1

公开(公告)日：2010-05-20

申请号：DE102008058068

申请日：2008-11-18

Applicant: LEICA MICROSYSTEMS

Inventor： SIEBER JOCHEN ,  DYBA MARCUS ,  SEYFRIED VOLKER

IPC: G01N21/64

Abstract: The device comprises a feeder device (4) for components (1,2) which are adjusted or regulated by the feeder device on the basis of a measurement data of the measurement parameter determined by a microscopic method. A microscope (5) is provided for determining the measurement data which is obtained by image analysis. The measurement parameter is intensity in a medium (3). An independent claim is included for a method for preparing a concentration of components in a fluid medium, particularly in a microscopic sample.

公开(公告)号：DE102008064164A1

公开(公告)日：2010-07-01

申请号：DE102008064164

申请日：2008-12-22

Applicant: LEICA MICROSYSTEMS

Inventor： SIEBER JOCHEN ,  SEYFRIED VOLKER

IPC: G01N33/58 ,  G01N21/64

Abstract: Ein Verfahren zur räumlich hochauflösenden stochastischen Untersuchung einer mit einer Substanz markierten Struktur einer Probe, wobei als Struktur, die mit der Substanz markiert wird, eine biologische Struktur verwendet wird, wobei Moleküle der Substanz in einem ersten Zustand (A) und in einem zweiten Zustand (B) vorliegen können, wobei sich der erste und der zweite Zustand (A, B) in mindestens einer fotophysikalischen Eigenschaft voneinander unterscheiden, ist im Hinblick auf eine besonders eierten Struktur einer Probe derart ausgestaltet und weitergebildet, dass eine Substanz verwendet wird, bei der mit hinreichend hoher Wahrscheinlichkeit und in hinreichend großem Umfang ein Teil der Moleküle in dem ersten Zustand (A) und ein Teil der Moleküle in dem zweiten Zustand (B) vorliegen und ein Wechsel zwischen den beiden Zuständen (A, B) in beiden Richtungen spontan erfolgen kann.

公开(公告)号：DE102008058088A1

公开(公告)日：2010-05-20

申请号：DE102008058088

申请日：2008-11-18

Applicant: LEICA MICROSYSTEMS

Inventor： SIEBER JOCHEN

IPC: G01N33/58 ,  G01N33/68

Abstract: The method for spatial high-resolution examination of a biological structure (1) of a sample marked with a substance (3), comprises converting the substance from a first state such as a non-fluorescent state into a second state such as a fluorescent state, where the first and second states differ from each other in a photo-physical property, initially binding a protein tag (2) on the structure for marking the structure or expressing the protein tag together with the structure as fusion protein, and then non-covalently binding the substance by the tag. The tag has fluorogen activating protein. The method for spatial high-resolution examination of a biological structure (1) of a sample marked with a substance (3), comprises converting the substance from a first state such as a non-fluorescent state into a second state such as a fluorescent state, where the first and second states differ from each other in a photo-physical property, initially binding a protein tag (2) on the structure for marking the structure or expressing the protein tag together with the structure as fusion protein, and then non-covalently binding the substance by the tag. The tag has fluorogen activating protein. The substance is only switchable or convertible from the first state into the second state in a state unbound with the fluorogen activating protein. A bleached molecule of the substance is substituted by an unbleached molecule of the substance during the state unbound with the fluorogen activating protein. The tag has a part binding the structure and a part binding the substance. The part binding the structure non-covalently binds to the part binding the substance. An adapter protein is realized between the part binding the structure and the part binding the substance. The photo-physical properties of the substance are influenced by providing or changing local environmental conditions. The change of the photo-physical properties of the substance takes place by binding or the type of binding to the fluorogen activating protein. The fluorogen activating protein-type is selected or adapted in dependent of the examination technique or property of the examination technique.