公开(公告)号：WO2011026141A3

公开(公告)日：2011-04-21

申请号：PCT/US2010047402

申请日：2010-08-31

Applicant: LIFE TECHNOLOGIES CORP ,  COX DAVID ,  HABERSTROH MARC ,  KINNEY PATRICK D ,  CARRILLO ALBERT L ,  HOSHIZAKI JON A ,  SHARIATI MARYAM ,  KING HOWARD G ,  LEE JOE Y ,  CHAN MATTHEW S

Inventor： COX DAVID ,  HABERSTROH MARC ,  KINNEY PATRICK D ,  CARRILLO ALBERT L ,  HOSHIZAKI JON A ,  SHARIATI MARYAM ,  KING HOWARD G ,  LEE JOE Y ,  CHAN MATTHEW S

IPC: G02B7/00 ,  G02B21/00

CPC classification number: C12Q1/6869 ,  B01F13/0059 ,  B01F13/0222 ,  B01J19/0046 ,  B01J2219/00317 ,  B01J2219/00466 ,  B01J2219/00527 ,  B01J2219/00576 ,  B01J2219/00596 ,  B01J2219/00648 ,  B01J2219/00659 ,  B01J2219/00722 ,  B01L3/502707 ,  B01L3/50273 ,  B01L2200/027 ,  B01L2200/0647 ,  B01L2200/0684 ,  B01L2200/16 ,  B01L2300/0819 ,  B01L2300/0822 ,  B01L2300/0867 ,  B01L2300/0877 ,  B01L2300/0887 ,  B01L2300/1822 ,  B01L2400/0427 ,  C12Q1/6874 ,  G01N35/00069 ,  G01N35/1002 ,  G01N35/1097 ,  G01N2035/00148 ,  G01N2035/00237 ,  G01N2035/1034 ,  G02B21/34 ,  Y10T436/11 ,  Y10T436/143333

Abstract: Various embodiments of a sequencing system capable of rapidly imaging samples at multiple wavelengths are provided herein. In one embodiment, the system includes a fast- indexing filter wheel having a plurality of excitation and emission filters capable of being rapidly rotated into and out of communication with an excitation source (e.g., an arc lamp, a laser). For example, the filter wheel can be configured to index in an amount of time falling within a range of about 40 ms to about 60 ms, preferably 50 ms. The system can also be configured to account for vibrations resulting from the quick starts and stops of the fast- indexing filter wheel as well as vibrations resulting from other sources. Various methods of rapidly imaging a sample at multiple wavelength are also provided herein.

Abstract translation: 本文提供了能够快速成像多个波长的样品的测序系统的各种实施例。 在一个实施例中，该系统包括快速分度滤光轮，其具有能够快速旋转进入激发源（例如，弧光灯，激光器）和与激光源（例如，弧光灯，激光器）连通的多个激发和发射滤光片。 例如，滤光轮可被配置成以大约40ms至大约60ms，优选为50ms的范围内的时间量进行索引。 该系统还可以配置为考虑快速启动和停止快速分度滤光轮以及其他来源产生振动的振动。 本文还提供了用于快速成像多个波长的样品的各种方法。

公开(公告)号：AT521006T

公开(公告)日：2011-09-15

申请号：AT05764213

申请日：2005-06-30

Applicant: LIFE TECHNOLOGIES CORP

Inventor： KINNEY PATRICK D ,  KING HOWARD G ,  PALLAS MICHAEL C ,  NALEY MARK

IPC: G02B21/24 ,  G02B7/38