公开(公告)号：WO2021092523A1

公开(公告)日：2021-05-14

申请号：PCT/US2020/059581

申请日：2020-11-07

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： LEONG, Harrison ,  BAUDO, Charles ,  HIGDON, Charles, Wendell

IPC: C12Q1/6886 ,  C12Q1/6851 ,  C12Q1/6827

Abstract: Systems and methods for detecting microsatellite instability in a biological sample are described. Signal data is received from a capillary electrophoresis genetic analysis instrument, wherein the signal data is measured from fluorescence of fragments comprising nucleic acid sequences amplified from the biological sample via polymerase chain reaction (PCR). The nucleic acid sequences correspond to a plurality of different microsatellite loci and are obtained using a plurality of PCR primers configured to flank a plurality of microsatellite loci of a biological sample. When the PCR primers and the biological sample are combined and subjected to PCR amplification, fluorescently labeled DNA fragments are generated comprising the plurality of microsatellite loci. Fluorescent data obtained from the plurality of fluorescently labelled microsatellite loci are used to classify microsatellite instability of the biological sample.

公开(公告)号：WO2021092299A1

公开(公告)日：2021-05-14

申请号：PCT/US2020/059295

申请日：2020-11-06

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： HIGDON, Charles ,  LEONG, Harrison ,  BAUDO, Charles ,  BORNARTH, Carole ,  SCHREIBER, Edgar

IPC: C12Q1/6886 ,  C12Q1/6851 ,  C12Q1/6827

Abstract: Systems, primers, kits, and methods for detecting microsatellite instability in a biological sample are described. Signal data is received from a capillary electrophoresis genetic analysis instrument, wherein the signal data is measured from fluorescence of fragments comprising nucleic acid sequences amplified from the biological sample via polymerase chain reaction (PCR). The nucleic acid sequences correspond to a plurality of different microsatellite loci and are obtained using a plurality of PCR primers configured to flank a plurality of microsatellite loci of a biological sample. When the PCR primers and the biological sample are combined and subjected to PCR amplification, fluorescently labeled DNA fragments are generated comprising the plurality of microsatellite loci. Fluorescent data obtained from the plurality of fluorescently labelled microsatellite loci are used to classify microsatellite instability of the biological sample.

公开(公告)号：EP4055195A1

公开(公告)日：2022-09-14

申请号：EP20817570.3

申请日：2020-11-07

Applicant: Life Technologies Corporation

Inventor： LEONG, Harrison ,  BAUDO, Charles ,  HIGDON, Charles, Wendell

IPC: C12Q1/6886 ,  C12Q1/6851 ,  C12Q1/6827