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    PIPPETTE DEVICES, SYSTEMS, AND METHODS OF USE THEREOF
    1.
    发明申请
    PIPPETTE DEVICES, SYSTEMS, AND METHODS OF USE THEREOF 审中-公开
    PIPPETTE设备，系统及其使用方法

    公开(公告)号：WO2013166203A2

    公开(公告)日：2013-11-07

    申请号：PCT/US2013/039142

    申请日：2013-05-01

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： JACKSON, John ,  BULLOCH, Kyle ,  KAHATT, Espir

    IPC: B01L3/02

    CPC classification number: B01L3/0217 ,  B01L3/022 ,  B01L3/0279 ,  B01L2200/025 ,  B01L2200/087 ,  C12M35/02

    Abstract: In some embodiments the present disclosure describes novel pipette devices, pipetting systems and methods of use thereof which require a uses to use less force for one or more functions such as to: load a pipette tip, aspirate sample, dispense sample and eject a pipette tip. A pipette device of the disclosure comprises: a pipette plunger; at least one pair of magnets; and at least one spring; wherein the pipette device configured to reduce the force required by a user to aspirate, dispense, load and eject a pipette tip. In some embodiments, a pipette device of the disclosure further comprises a detent. In some embodiments a pipette according to the disclosure further comprises a trigger. Some embodiments describe electroporation systems comprising a pipette device of the disclosure as well as various configurations of electroporation systems and devices and methods of use thereof.

    Abstract translation: 在一些实施方案中，本公开描述了新颖的移液管装置，移液系统及其使用方法，其需要使用较少的力用于一种或多种功能，例如：装载移液管吸头，抽吸样品，分配样品并喷射移液管吸头 。 本公开的移液管装置包括：移液管柱塞; 至少一对磁铁; 和至少一个弹簧; 其中所述移液管装置被配置成减小使用者抽吸，分配，加载和喷射移液管尖端所需的力。 在一些实施例中，本公开的移液管装置还包括棘爪。 在一些实施例中，根据本公开的移液器还包括触发器。 一些实施方案描述了电穿孔系统，其包括本公开的吸移管装置以及电穿孔系统和装置的各种构造及其使用方法。

    PREPARATION OF ELECTROPHORESIS GELS, AND RELATED DEVICES, SYSTEMS, AND METHODS
    2.
    发明申请
    PREPARATION OF ELECTROPHORESIS GELS, AND RELATED DEVICES, SYSTEMS, AND METHODS 审中-公开
    电泳凝胶的制备及相关器件，系统和方法

    公开(公告)号：WO2017083609A1

    公开(公告)日：2017-05-18

    申请号：PCT/US2016/061476

    申请日：2016-11-11

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： BULLOCH, Kyle ,  DILLER, Thomas

    IPC: G01N27/447

    CPC classification number: G01N27/44747 ,  G01N27/44704 ,  G01N27/44782

    Abstract: An apparatus (200) for preparation of an electrophoresis slab gel may include a base (220) having an opening configured to receive a cassette (100) configured to contain an electrophoresis slab gel, a clamping mechanism (240) configured to move relative to the base (220) between an open position in which the clamping mechanism (240) permits insertion of a cassette into the base (220), and a closed position in which the clamping mechanism (240) is configured to clamp a cassette received in the base, a compressible pad (260) operatively coupled to the clamping mechanism in a position to compress against a cassette (100) received in the base (220) in the closed position of the clamping mechanism (240). The cassette (100) may include a first plate (11) and a second plate (12), and a spacer mechanism (13) separate from each of the first and second plates, the spacer mechanism configured to be positioned between the inner faces of the first plate and the second plate. When subjected to a clamping force exerted on the outer faces of the first and second plates, the spacer mechanism is configured to maintain a separation distance between the inner faces of the first and second plates, and provide a seal to prevent leakage of an electrophoresis gel solution introduced between the plates.

    Abstract translation: 用于制备电泳平板凝胶的装置（200）可以包括：具有开口的基座（220），所述开口构造成接收配置为容纳电泳平板凝胶的盒（100）;夹紧机构（ 240），所述闭合位置构造成相对于所述基座（220）在所述夹紧机构（240）允许将盒子插入所述基座（220）的打开位置与所述夹紧机构（240）配置于其中的闭合位置之间移动 以夹紧容纳在底座中的盒子;在夹紧机构（240）的关闭位置中，可操作地联接到夹紧机构的可压缩垫（260）压靠容纳在基座（220）中的盒子（100） 。 盒（100）可包括第一板（11）和第二板（12）以及与第一板和第二板中的每一个分离的间隔机构（13），间隔机构构造成定位在第一板 第一板和第二板。 当受到施加在第一和第二板的外表面上的夹紧力时，间隔机构被构造成保持第一和第二板的内表面之间的分离距离，并且提供密封以防止电泳凝胶的泄漏 解决方案介绍之间的板块。

    PREPARATION OF ELECTROPHORESIS GELS, AND RELATED DEVICES, SYSTEMS, AND METHODS
    4.
    发明公开
    PREPARATION OF ELECTROPHORESIS GELS, AND RELATED DEVICES, SYSTEMS, AND METHODS 审中-公开

    公开(公告)号：EP3374764A1

    公开(公告)日：2018-09-19

    申请号：EP16804964.1

    申请日：2016-11-11

    Applicant: Life Technologies Corporation

    Inventor： BULLOCH, Kyle ,  DILLER, Thomas

    IPC: G01N27/447

    CPC classification number: G01N27/44747 ,  G01N27/44704 ,  G01N27/44782

    Abstract: An apparatus for preparation of an electrophoresis slab gel may include a base having an opening configured to receive a cassette configured to contain an electrophoresis slab gel, a clamping mechanism configured to move relative to the base between an open position in which the clamping mechanism permits insertion of a cassette into the base, and a closed position in which the clamping mechanism is configured to clamp a cassette received in the base, a compressible pad operatively coupled to the clamping mechanism in a position to compress against a cassette received in the base in the closed position of the clamping mechanism. The cassette may include a first plate and a second plate, and a spacer mechanism separate from each of the first and second plates, the spacer mechanism configured to be positioned between the inner faces of the first plate and the second plate. When subjected to a clamping force exerted on the outer faces of the first and second plates, the spacer mechanism is configured to maintain a separation distance between the inner faces of the first and second plates, and provide a seal to prevent leakage of an electrophoresis gel solution introduced between the plates.

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