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    COMPOSITIONS AND METHODS FOR DETECTION OF MULTIPLE MICROORGANISMS
    3.
    发明申请
    COMPOSITIONS AND METHODS FOR DETECTION OF MULTIPLE MICROORGANISMS 审中-公开
    用于检测多种微生物的组合物和方法

    公开(公告)号：WO2014003997A1

    公开(公告)日：2014-01-03

    申请号：PCT/US2013/044180

    申请日：2013-06-04

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： TEBBS, Robert ,  CUMMINGS, Craig ,  BRZOSKA, Pius ,  MATHENY, Sharon ,  O'CONNELL, Catherine ,  FURTADO, Manohar ,  SERGEEV, Nikolay ,  BREVNOV, Maxim

    IPC: C12Q1/68

    CPC classification number: C12Q1/689 ,  C12Q2600/16

    Abstract: The present disclosure describes compositions, methods and kits for detection of one or multiple microorganism contaminants in samples. Some embodiments relate to detecting one or more microorganisms producing virulence factors such as shiga toxin stx1 and stx2 and eae. Some embodiments relate to detection of STEC microorganisms including an E. coli O26, an E. coli O45, an E. coli O103, an E. coli O111, an E. coli O121 or an E. coli O145. In some embodiments, compositions, methods and kits can detect and identify individual serotypes of shiga toxin producing microorganisms. Workflows for multiple microbe detection and identification are also described.

    Abstract translation: 本公开描述了用于检测样品中一种或多种微生物污染物的组合物，方法和试剂盒。 一些实施方案涉及检测产生毒力因子的一种或多种微生物，例如志贺毒素stx1和stx2和eae。 一些实施方案涉及检测STEC微生物，包括大肠杆菌O26，大肠杆菌O45，大肠杆菌O103，大肠杆菌O111，大肠杆菌O121或大肠杆菌O145。 在一些实施方案中，组合物，方法和试剂盒可以检测和鉴定产生志贺毒素的微生物的个体血清型。 还描述了用于多个微生物检测和鉴定的工作流程。

    COMPOSITIONS AND METHODS FOR DETECTION OF MYCOBACTERIUM AVIUM PARATUBERCULOSIS
    4.
    发明申请
    COMPOSITIONS AND METHODS FOR DETECTION OF MYCOBACTERIUM AVIUM PARATUBERCULOSIS 审中-公开
    用于检测艾滋病病原菌的组合物和方法

    公开(公告)号：WO2013151647A1

    公开(公告)日：2013-10-10

    申请号：PCT/US2013/029072

    申请日：2013-03-05

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： JI, Yongmei ,  BRZOSKA, Pius ,  BURRELL, Angela ,  CUMMINGS, Craig ,  O´CONNELL, Caytherine

    IPC: C12Q1/68

    CPC classification number: C12Q1/689 ,  C12Q1/6806 ,  C12Q2600/112 ,  C12Q2600/16

    Abstract: Disclosed are compositions, assays, methods, diagnostic methods, kits and diagnostic kits for the specific and differential detection of Mycobacterium avium subsp. paratuberculosis from samples including veterinary samples, clinical samples, food samples, forensic sample, an environmental sample (e.g., soil, dirt, garbage, sewage, air, or water), including food processing and manufacturing surfaces, or a biological sample.

    Abstract translation: 公开了用于特异性和差异性检测鸟分枝杆菌亚种的组合物，测定法，方法，诊断方法，试剂盒和诊断试剂盒。 来自包括兽医样品，临床样品，食品样品，法医样品，环境样品（例如土壤，污垢，垃圾，污水，空气或水）的样品中的副结核病，包括食品加工和制造表面或生物样品。

    DISCRIMINATORY POSITIVE/EXTRACTION CONTROL DNA
    7.
    发明申请
    DISCRIMINATORY POSITIVE/EXTRACTION CONTROL DNA 审中-公开
    歧视积极/提取控制DNA

    公开(公告)号：WO2010111509A1

    公开(公告)日：2010-09-30

    申请号：PCT/US2010/028693

    申请日：2010-03-25

    Applicant: LIFE TECHNOLOGIES CORPORATION ,  BREWER, Michael ,  PETRAUSKENE, Olga ,  LIU, Jen-Kuei ,  CUMMINGS, Craig ,  LIEW, Sueh-Ning ,  FURTADO, Manohar

    Inventor： BREWER, Michael ,  PETRAUSKENE, Olga ,  LIU, Jen-Kuei ,  CUMMINGS, Craig ,  LIEW, Sueh-Ning ,  FURTADO, Manohar

    IPC: C12Q1/68

    CPC classification number: C12Q1/6851 ,  C12Q2561/107 ,  C12Q2545/107 ,  C12Q2537/107

    Abstract: The present teachings generally relate to methods and kits incorporating a discriminating positive control for determining whether a particular microorganism or group of microorganisms are present in a sample, for example but not limited to a food, environmental, agricultural, biopharmaceutical, pharmaceutical, or water sample. According to certain methods, at least part of a starting material, for example but not limited to, a food, environmental, agricultural, biopharmaceutical, pharmaceutical, or water sample can be combined with a culture medium and incubated under conditions suitable for microbial growth followed by extracting microorganism and added control nucleic acids for analysis. The extracted nucleic acids are amplified and the amplified nucleic acids are detected, directly or indirectly, and the fidelity of the methods and the presence or absence of the corresponding microorganism is determined because the discriminating positive control provides both confirmatory results for the methods used but eliminates false positive results.

    Abstract translation: 本教导通常涉及包含用于确定特定微生物或一组微生物是否存在于样品中的鉴别性阳性对照的方法和试剂盒，例如但不限于食品，环境，农业，生物制药，药物或水样品 。 根据某些方法，至少部分原料，例如但不限于食品，环境，农业，生物制药，药物或水样品可以与培养基组合，并在适合于微生物生长的条件下温育 通过提取微生物和添加的对照核酸进行分析。 所提取的核酸被扩增并且直接或间接检测扩增的核酸，并且确定方法的保真度和相应的微生物的存在或不存在，因为鉴别性阳性对照提供所使用的方法的确认结果但消除 假阳性结果。

    COMPOSITIONS AND METHODS FOR DETECTION OF MICROORGANISMS OF THE MYCOBACTERIUM AVIUM COMPLEX EXCLUDING MYCOBACTERIUM AVIUM PARATUBERCULOSIS
    8.
    发明申请
    COMPOSITIONS AND METHODS FOR DETECTION OF MICROORGANISMS OF THE MYCOBACTERIUM AVIUM COMPLEX EXCLUDING MYCOBACTERIUM AVIUM PARATUBERCULOSIS 审中-公开
    用于检测MYCOBACTERIUM AVIUM复合物微生物的组合物和方法，不包括MYCOBACTERIUM AVIUM PARATUBERCULOSIS

    公开(公告)号：WO2013151648A1

    公开(公告)日：2013-10-10

    申请号：PCT/US2013/029093

    申请日：2013-03-05

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： CUMMINGS, Craig ,  BRZOSKA, Pius ,  BURRELL, Angela ,  O'CONNELL, Catherine

    IPC: C12Q1/68

    CPC classification number: C12Q1/689 ,  C12Q2600/112

    Abstract: Disclosed are compositions, assays, methods, diagnostic methods, kits and diagnostic kits for the specific and differential detection of a non-Mycobacterium avium subsp. paratuberculosis (non-MAP) organism, wherein a non-MAP organism is a Mycobacterium avium complex (MAC) organism that does not belong to the Mycobacterium avium subsp. paratuberculosis (MAP) organism, from samples including veterinary samples, clinical samples, food samples, forensic sample, an environmental sample (e.g., soil, dirt, garbage, sewage, air, or water), including food processing and manufacturing surfaces, or a biological sample. Exemplary non-MAP organisms including M. avium subsp. avium (MAA), M. avium subsp. hominissuis (MAH), and M. avium subsp. silvaticum (MAS) can be detected by the present compositions, kits and methods.

    Abstract translation: 公开了用于特异性和差异性检测非分枝杆菌鸟属亚种的组合物，测定，方法，诊断方法，试剂盒和诊断试剂盒。 副结核病（非MAP）生物体，其中非MAP生物体是不属于鸟分枝杆菌亚种的鸟分枝杆菌复合体（MAC）生物体。 来自包括兽医样品，临床样品，食品样品，法医样品，环境样品（如土壤，污垢，垃圾，污水，空气或水）在内的样品的副结核病（MAP）生物，包括食品加工和制造表面，或 生物样品。 示例性非MAP生物体，包括鸟分枝杆菌 鸟类（MAA），鸟分枝杆菌 山楂（MAH）和鸟分枝杆菌 可以通过本发明的组合物，试剂盒和方法检测silvaticum（MAS）。

    COMPOSITIONS AND METHODS FOR DETECTING AND IDENTIFYING SALMONELLA ENTERICA STRAINS
    9.
    发明申请
    COMPOSITIONS AND METHODS FOR DETECTING AND IDENTIFYING SALMONELLA ENTERICA STRAINS 审中-公开
    用于检测和鉴定沙门氏菌菌丝菌株的组合物和方法

    公开(公告)号：WO2012145557A1

    公开(公告)日：2012-10-26

    申请号：PCT/US2012/034318

    申请日：2012-04-19

    Applicant: LIFE TECHNOLOGIES CORPORATION ,  CUMMINGS, Craig ,  BOLCHAKOVA, Elena ,  FURTADO, Manohar

    Inventor： CUMMINGS, Craig ,  BOLCHAKOVA, Elena ,  FURTADO, Manohar

    IPC: C12Q1/68

    CPC classification number: C12Q1/689 ,  C12N15/1089 ,  C12Q2600/156 ,  C12Q2600/158 ,  Y02A50/451

    Abstract: The present specification describes several novel SNPs of Salmonella enterica subsp. enterica. SNP profiles comprising allelic compositions at each SNP position are described which may be used to identify and differentiate different strains and serovars of Salmonella enterica subsp. enterica. The specification also describes several compositions, methods and kits useful for identifying and differentially distinguishing strains and serovars of Salmonella enterica subsp. enterica.

    Abstract translation: 本说明书描述了几种新型的肠炎沙门氏菌的SNP。 肠炎。 描述了包含每个SNP位置的等位基因组成的SNP谱，其可用于鉴定和区分不同的肠炎沙门氏菌菌株和血清型。 肠炎。 该说明书还描述了用于鉴定和差异区分肠炎沙门氏菌菌株和血清型的几种组合物，方法和试剂盒。 肠炎。

    DETECTION OF LISTERIA SPECIES IN FOOD AND ENVIRONMENTAL SAMPLES, METHODS AND COMPOSITIONS THEREOF
    10.
    发明申请
    DETECTION OF LISTERIA SPECIES IN FOOD AND ENVIRONMENTAL SAMPLES, METHODS AND COMPOSITIONS THEREOF 审中-公开
    食品和环境样品中的LISTERIA物种的检测方法及其组合物

    公开(公告)号：WO2012003409A1

    公开(公告)日：2012-01-05

    申请号：PCT/US2011/042720

    申请日：2011-06-30

    Applicant: LIFE TECHNOLOGIES CORPORATION ,  PETRAUSKENE, Olga ,  CUMMINGS, Craig ,  VATTA, Paolo ,  TEBBS, Robert ,  BALACHANDRAN, Priya ,  ZODER, Patrick ,  WONG, Lily

    Inventor： PETRAUSKENE, Olga ,  CUMMINGS, Craig ,  VATTA, Paolo ,  TEBBS, Robert ,  BALACHANDRAN, Priya ,  ZODER, Patrick ,  WONG, Lily

    IPC: C12Q1/68

    CPC classification number: C12Q1/689 ,  C12Q2600/156 ,  C12Q2600/16

    Abstract: Embodiments of the disclosure relate to isolated nucleic acid sequences, methods of use thereof, and workflows for detection of several Listeria species in a sample, particularly in a food or environmental sample. Embodiments of the disclosure may also be used to detect one or more species or strains of Listeria from each other, for example L. grayi may be detected independently of other Listeria spp . Some embodiments also describe a duplexed assay that can detect L. monocytogenes, L. innocua, L. welshimeri, L. seelgeri, L. marthii (formerly incertae-sedis), L. ivanovii, and L. grayi . Kits for detection of Listeria are also described. In some embodiments, methods and kits of the disclosure may comprise a TAQMAN® assay. In some embodiments, 0.2-2 cfu of Listeria spp. are detected using the compositions, methods and kits after a 24-28 hour enrichment period.

    Abstract translation: 本公开的实施方案涉及分离的核酸序列，其使用方法和用于检测样品中的几种利斯特氏菌物种的工作流程，特别是在食品或环境样品中。 本公开的实施方案还可以用于彼此检测李斯特氏菌的一种或多种物种或菌株，例如，可以独立于其他李斯特菌属物种检测L.crilli。 一些实施方案还描述了可以检测单核细胞增生李斯特氏菌（L. innocua），威尔逊氏乳杆菌（L.Welshimeri），塞贝尔氏球菌（L.ceelgeri），马氏体（L.marthii）（以前称为incertae-sedis），伊马诺病毒L. 还描述了用于检测李斯特菌的试剂盒。 在一些实施方案中，本公开的方法和试剂盒可以包括TAQMAN测定。 在一些实施方案中，0.2-2cfu的李斯特菌属 在24-28小时浓缩期后使用组合物，方法和试剂盒检测。

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