公开(公告)号：US20190154696A1

公开(公告)日：2019-05-23

申请号：US15753454

申请日：2016-08-30

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Scott CLARKE ,  Kyle GEE ,  Carmen FINNESSY

IPC: G01N33/58 ,  C12Q1/6813 ,  G01N33/52

Abstract: The present disclosure provides methods, compositions and kits for measuring cellular nascent nucleic acid synthesis by colorimetric labeling of nucleic acid. The nucleic acid synthesis can be measured as cell proliferation, DNA or RNA synthesis, gene expression or apoptosis. Additionally, these methods may be used to screen compounds for their effect on cellular proliferation by treating cells or an organism with the test compound simultaneous to or before treatment with a competitive nucleoside analog.