公开(公告)号：US20240110063A1

公开(公告)日：2024-04-04

申请号：US18345621

申请日：2023-06-30

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Andrew LEVITZ ,  Khairuzzaman Bashar MULLAH ,  Brian EVANS ,  Scott C. BENSON ,  Chris VONNEGUT

IPC: C09B57/14 ,  C12Q1/6818 ,  C12Q1/6844 ,  G01N29/24

CPC classification number: C09B57/14 ,  C12Q1/6818 ,  C12Q1/6844 ,  G01N29/2418

Abstract: The present disclosure relates to dibenzoxanthene compounds that are efficient quenchers of fluorescence, for example in the far red and near infrared spectrum. Applications using the dibenzoxanthene quenching compounds and methods of making same are also described.

公开(公告)号：US20200224249A1

公开(公告)日：2020-07-16

申请号：US16649088

申请日：2018-10-04

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Chris VONNEGUT ,  Scott CLARKE

IPC: C12Q1/6809

Abstract: Disclosed is a method for determining the quality, expressed in terms of a quality value, of an RNA sample based on a multiplex fluorescent ratiometric method, wherein the ratio of small RNA to large and/or structured RNA in a sample is determined, thereby obtaining the quality value. Also disclosed is a method for determining the quality value of an RNA sample wherein the relationship of the intensity of the fluorescent signal corresponding to small RNA is compared to the intensity of the fluorescent signal corresponding to large and/or structured RNA in a sample. The relationship of the intensity of the two fluorescent signals is used to determine the amount of intact RNA present in the sample.