公开(公告)号：WO2016100895A1

公开(公告)日：2016-06-23

申请号：PCT/US2015/066836

申请日：2015-12-18

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： MOZHAYSKIY, Vadim ,  FU, Yutao ,  HUBBELL, Earl

IPC: C12Q1/68

CPC classification number: C12Q1/6874 ,  C12N15/1058 ,  C12Q1/6825 ,  C12Q2525/173 ,  C12Q2525/204 ,  C12Q2535/122 ,  C12Q2545/101 ,  C12Q2565/518 ,  C12Q2565/607

Abstract: A method for preparing a homopolymer recalibration panel includes: extracting, from a set of amplicons used in sequencing-by-synthesis, a set of candidate amplicons satisfying a first set of criteria, wherein the first set of criteria includes amplicons known to belong to high-confidence regions of a reference genome with no variants; and selecting, from the set of candidate amplicons, a reduced set of amplicons satisfying a second set of criteria, wherein the second set of criteria includes amplicons that together comprise at least a minimal threshold number of homopolymers of each homopolymer length between a predetermined minimal homopolymer length and a predetermined maximal homopolymer length for one or more of homopolymer types A, T, C, and G.

Abstract translation: 制备均聚物重校准面板的方法包括：从一组合成中使用的扩增子提取一组满足第一组标准的候选扩增子，其中第一组标准包括已知属于高分子的扩增子 没有变体的参考基因组的自信区域; 以及从所述候选扩增子集合中选择满足第二组标准的缩减的扩增子集，其中所述第二组标准包括一起包括至少最小阈值数量的预定最小均聚物之间的每个均聚物长度的均聚物的最小阈值数的扩增子 长度和一种或多种均聚物类型A，T，C和G的预定最大均聚物长度。

公开(公告)号：EP3234198A1

公开(公告)日：2017-10-25

申请号：EP15871217.4

申请日：2015-12-18

Applicant: Life Technologies Corporation

Inventor： MOZHAYSKIY, Vadim ,  FU, Yutao ,  HUBBELL, Earl

IPC: C12Q1/68

CPC classification number: C12Q1/6874 ,  C12N15/1058 ,  C12Q1/6825 ,  C12Q2525/173 ,  C12Q2525/204 ,  C12Q2535/122 ,  C12Q2545/101 ,  C12Q2565/518 ,  C12Q2565/607

Abstract: A method for preparing a homopolymer recalibration panel includes: extracting, from a set of amplicons used in sequencing-by-synthesis, a set of candidate amplicons satisfying a first set of criteria, wherein the first set of criteria includes amplicons known to belong to high-confidence regions of a reference genome with no variants; and selecting, from the set of candidate amplicons, a reduced set of amplicons satisfying a second set of criteria, wherein the second set of criteria includes amplicons that together comprise at least a minimal threshold number of homopolymers of each homopolymer length between a predetermined minimal homopolymer length and a predetermined maximal homopolymer length for one or more of homopolymer types A, T, C, and G.

Abstract translation: 用于制备均聚物再校准面板的方法包括：从用于合成测序的一组扩增子中提取满足第一组标准的一组候选扩增子，其中第一组标准包括已知属于高位的扩增子 没有变异的参考基因组的置信区域; 并从候选扩增子组中选择满足第二组标准的扩增子集合，其中第二组标准包括一起包含至少最小阈值数量的每个均聚物长度的均聚物在预定的最小均聚物之间的扩增子 长度和一种或多种均聚物类型A，T，C和G的预定最大均聚物长度。