公开(公告)号：WO2011143478A2

公开(公告)日：2011-11-17

申请号：PCT/US2011/036320

申请日：2011-05-12

Applicant: LIFE TECHNOLOGIES CORPORATION ,  SERGEEV, Nikolay V. ,  BREVNOV, Maxim G. ,  FURTADO, Manohar R.

Inventor： SERGEEV, Nikolay V. ,  BREVNOV, Maxim G. ,  FURTADO, Manohar R.

IPC: C12Q1/68 ,  C12N15/11 ,  G01N33/58

CPC classification number: C12Q1/6853 ,  C12Q1/6818 ,  C12Q1/686 ,  Y02A50/52 ,  C12Q2565/1025 ,  C12Q2563/173 ,  C12Q2527/107 ,  C12Q2561/101

Abstract: This disclosure relates to methods for identifying target nucleic acids in a sample by detecting an amplified sequence corresponding to the target using a detectable probe and by monitoring its melting temperature (T m ).

Abstract translation: 本公开涉及通过使用可检测的探针检测对应于靶的扩增序列并通过监测其解链温度（Tm）来鉴定样品中的靶核酸的方法。

公开(公告)号：WO2023069604A1

公开(公告)日：2023-04-27

申请号：PCT/US2022/047259

申请日：2022-10-20

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： KONG, Say Li ,  LEOW, San Min ,  YING, Andy ,  FURTADO, Manohar R. ,  WONG, Grace Yu Hui ,  KENNY, Daryn ,  PHUA, Jacelyn Hong Yi ,  XU, Xiaohan

IPC: C12Q1/6851 ,  G16B40/10

Abstract: Disclosed are compositions, kits, and methods for amplifying and quantifying a target nucleic acid from a sample. Compositions, kits, and methods enable the quantification of a target nucleic acid from a sample using an internal quantification standard disposed in the same reaction volume as the target nucleic acid, thereby eliminating the need for additional amplification reactions and processing to generate a standard curve. Compositions, kits, and methods also enable the comparison of target nucleic acid loads between two or more test samples by normalizing measured levels of the target nucleic acid in each sample according to relative levels of endogenous nucleic acid in each test sample.

公开(公告)号：EP2569449A2

公开(公告)日：2013-03-20

申请号：EP11781302.2

申请日：2011-05-12

Applicant: Life Technologies Corporation

Inventor： SERGEEV, Nikolay V. ,  BREVNOV, Maxim G. ,  FURTADO, Manohar R.

IPC: C12Q1/68 ,  C12N15/11 ,  G01N33/58

CPC classification number: C12Q1/6853 ,  C12Q1/6818 ,  C12Q1/686 ,  Y02A50/52 ,  C12Q2565/1025 ,  C12Q2563/173 ,  C12Q2527/107 ,  C12Q2561/101

Abstract: This disclosure relates to methods for identifying target nucleic acids in a sample by detecting an amplified sequence corresponding to the target using a detectable probe and by monitoring its melting temperature (Tm).

Abstract translation: 本公开涉及用于通过使用可检测探针检测对应于靶的扩增序列并且通过监测其解链温度（Tm）来鉴定样品中的靶核酸的方法。

公开(公告)号：EP4419712A1

公开(公告)日：2024-08-28

申请号：EP22812874.0

申请日：2022-10-20

Applicant: Life Technologies Corporation ,  Life Technologies Holdings PTE LTD

Inventor： KONG, Say Li ,  LEOW, San Min ,  YING, Andy ,  FURTADO, Manohar R. ,  WONG, Grace Yu Hui ,  KENNY, Daryn ,  PHUA, Jacelyn Hong Yi ,  XU, Xiaohan

IPC: C12Q1/6851 ,  G16B40/10

CPC classification number: C12Q1/6851