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公开(公告)号:US20200277580A1
公开(公告)日:2020-09-03
申请号:US16850629
申请日:2020-04-16
Applicant: Life Technologies Corporation
Inventor: Peter VANDER HORN , Theo NIKIFOROV , Guobin LUO , Mindy LANDES , Daniel MAZUR , Eileen TOZER , Tommie LINCECUM
IPC: C12N9/12 , C12Q1/6844 , C12Q1/686
Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragment thereof are provided that allow for nucleic acid amplification. In one aspect, the disclosure relates to modified polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In some aspects, the disclosure relates to modified polymerases useful for the generation of nucleic acid libraries or nucleic acid templates for use in various downstream processes. In some aspects, the disclosure relates to the identification of homologous amino acid mutations that can be transferred across classes or families of polymerases to provide novel polymerases with altered catalytic properties. In some aspects, the disclosure provides modified polymerases having enhanced catalytic properties as compared to a reference polymerase.
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公开(公告)号:US20170369857A1
公开(公告)日:2017-12-28
申请号:US15690945
申请日:2017-08-30
Applicant: Life Technologies Corporation
Inventor: Peter VANDER HORN , Cheng-Yao CHEN , Guobin LUO , Michael PREVITE , Jamshid TEMIROV , Theo NIKIFOROV , Zhaohui ZHOU , Hongye SUN , Yufang WANG , Stefanie Yukiko NISHIMURA , Hongyi WANG , Marian PERIS , Barnett ROSENBLUM , Michael PHELAN
CPC classification number: C12N9/1252 , C12Q1/68 , C12Q1/6804 , C12Q1/6818 , C12Q1/6827 , C12Q1/6869 , C12Q1/6872 , C12Y207/07007 , G01N2500/00 , C12Q2565/632 , C12Q2563/137 , C12Q2563/107 , C12Q2565/30 , C12Q2537/157
Abstract: Provided herein are compositions and systems for use in polymerase-dependent, nucleotide transient-binding methods. The methods are useful for deducing the sequence of a template nucleic acid molecule and single nucleotide polymorphism (SNP) analyses. The methods rely on the fact that the polymerase transient-binding time for a complementary nucleotide is longer compared to that of a non-complementary nucleotide. The labeled nucleotides transiently-binds the polymerase in a template-dependent manner, but does not incorporate. The methods are conducted under any reaction condition that permits transient binding of a complementary or non-complementary nucleotide to a polymerase, and inhibits nucleotide incorporation.
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公开(公告)号:US20240101980A1
公开(公告)日:2024-03-28
申请号:US18521466
申请日:2023-11-28
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor: Daniel MAZUR , Sihong CHEN , Peter VANDER HORN , Eileen TOZER , Guobin LUO , Joshua SHIRLEY , Kevin HEINEMANN
CPC classification number: C12N9/1252 , C12Q1/686 , C12Y207/07007
Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, recombinant polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. In some aspects, the disclosure provides recombinant polymerases that yield lower systematic error rates and/or improved accuracy, when used in sequencing by synthesis reactions as compared to a control polymerase. In one aspect, the disclosure relates to recombinant polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In another aspect, the recombinant polymerases are useful for the amplification of nucleic acid templates during PCR, emPCR, isothermal amplification, recombinase polymerase amplification, rolling circle amplification, strand displacement amplification and proximity ligation amplification. In some aspects, the disclosure relates to recombinant polymerases useful for the generation of nucleic acid libraries and/or nucleic acid templates.
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公开(公告)号:US20200017846A1
公开(公告)日:2020-01-16
申请号:US16542121
申请日:2019-08-15
Applicant: Life Technologies Corporation
Inventor: John DAVIDSON , Theo NIKIFOROV , Guobin LUO
Abstract: In some embodiments, the disclosure relates generally to methods as well as related compositions, systems, kits and apparatus comprising linking proteins to target compounds and/or to locations of interest using tethers. For example, the tether can be used to link the protein to a target compound, for example, to link an enzyme to a substrate. Similarly, the tether can be used to link the protein at or near a desired location on a surface. In one group of embodiments, the tether includes a polynucleotide and the target compound or location on the surface includes another polynucleotide that is capable of hybridizing to the tether. In such embodiments, the tether can be used to link the protein to the target compound or location using nucleic acid hybridization.
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公开(公告)号:US20220162573A1
公开(公告)日:2022-05-26
申请号:US17645146
申请日:2021-12-20
Applicant: Life Technologies Corporation
Inventor: Peter VANDER HORN , Theo NIKIFOROV , Guobin LUO , Mindy LANDES , Daniel MAZUR , Eileen TOZER , Tommie LINCECUM
IPC: C12N9/12 , C12Q1/6844 , C12Q1/686
Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragment thereof are provided that allow for nucleic acid amplification. In one aspect, the disclosure relates to modified polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In some aspects, the disclosure relates to modified polymerases useful for the generation of nucleic acid libraries or nucleic acid templates for use in various downstream processes. In some aspects, the disclosure relates to the identification of homologous amino acid mutations that can be transferred across classes or families of polymerases to provide novel polymerases with altered catalytic properties. In some aspects, the disclosure provides modified polymerases having enhanced catalytic properties as compared to a reference polymerase.
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公开(公告)号:US20210284976A1
公开(公告)日:2021-09-16
申请号:US17302621
申请日:2021-05-07
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor: Daniel MAZUR , Peter B. VANDER HORN , Eileen TOZER , Sihong CHEN , Guobin LUO , Joshua SHIRLEY , Kevin HEINEMANN
Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, recombinant polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. In some aspects, the disclosure provides recombinant polymerases that yield lower systematic error rates and/or improved accuracy, when used in sequencing by synthesis reactions as compared to a control polymerase. In one aspect, the disclosure relates to recombinant polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In another aspect, the recombinant polymerases are useful for the amplification of nucleic acid templates during PCR, emPCR, isothermal amplification, recombinase polymerase amplification, rolling circle amplification, strand displacement amplification and proximity ligation amplification. In some aspects, the disclosure relates to recombinant polymerases useful for the generation of nucleic acid libraries and/or nucleic acid templates.
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公开(公告)号:US20180223357A1
公开(公告)日:2018-08-09
申请号:US15785579
申请日:2017-10-17
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor: Guobin LUO , Marina SEDOVA , David LIGHT , Ryan JONES , Mohammad ALANJARY
IPC: C12Q1/6874 , C12Q1/6806
CPC classification number: C12Q1/6874 , C12Q1/6806 , C12Q2527/125 , C12Q2563/149 , C12Q2563/137 , C12Q2535/122
Abstract: A method of sequencing a nucleic acid strand includes receiving particles having nucleic acid strands coupled to a polymer matrix, exposing the particles to a solution including a condensing agent, and applying the particles to a surface, the particles depositing on the surface.
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公开(公告)号:US20140234853A1
公开(公告)日:2014-08-21
申请号:US14108166
申请日:2013-12-16
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor: Peter VANDER HORN , Cheng-Yao CHEN , Guobin LUO , Michael PREVITE , Jamshid TEMIROV , Theo NIKIFOROV , Zhaohui ZHOU , Hongye SUN , Yufang WANG , Stefanie Yukiko NISHIMURA , Hongyi WANG , Marian PERIS , Barnett ROSENBLUM , Michael PHELAN
IPC: C12Q1/68
CPC classification number: C12N9/1252 , C12Q1/68 , C12Q1/6804 , C12Q1/6818 , C12Q1/6827 , C12Q1/6869 , C12Q1/6872 , C12Y207/07007 , G01N2500/00 , C12Q2565/632 , C12Q2563/137 , C12Q2563/107 , C12Q2565/30 , C12Q2537/157
Abstract: Provided herein are compositions and systems for use in polymerase-dependent, nucleotide transient-binding methods. The methods are useful for deducing the sequence of a template nucleic acid molecule and single nucleotide polymorphism (SNP) analyses. The methods rely on the fact that the polymerase transient-binding time for a complementary nucleotide is longer compared to that of a non-complementary nucleotide. The labeled nucleotides transiently-binds the polymerase in a template-dependent manner, but does not incorporate. The methods are conducted under any reaction condition that permits transient binding of a complementary or non-complementary nucleotide to a polymerase, and inhibits nucleotide incorporation.
Abstract translation: 本文提供了用于聚合酶依赖性核苷酸瞬变结合方法的组合物和系统。 该方法可用于推导模板核酸分子和单核苷酸多态性(SNP)分析的序列。 该方法依赖于与非互补核苷酸相比,互补核苷酸的聚合酶瞬时结合时间更长的事实。 标记的核苷酸以模板依赖的方式瞬时结合聚合酶,但不包括在内。 所述方法在允许互补或非互补核苷酸与聚合酶短暂结合并且抑制核苷酸掺入的任何反应条件下进行。
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公开(公告)号:US20240318248A1
公开(公告)日:2024-09-26
申请号:US18733283
申请日:2024-06-04
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor: Guobin LUO , Marina Sedova , David Light , Ryan Jones , Mohammad Alanjary
IPC: C12Q1/6874 , C12Q1/6806
CPC classification number: C12Q1/6874 , C12Q1/6806
Abstract: A method for depositing particles on a surface includes receiving a plurality of particles, a particle of the plurality of particles having a polymer matrix conjugated to nucleic acid strands, exposing the plurality of particles to a solution; and applying the plurality of particles to a surface following exposing the plurality of particles to the solution, particles of the plurality of particles depositing on the surface. The solution includes a magnesium salt in a range of 30 mM to 500 mM and polyethylene glycol.
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公开(公告)号:US20220315917A1
公开(公告)日:2022-10-06
申请号:US17302674
申请日:2021-05-10
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor: Mark ANDERSEN , Daniel MAZUR , Sihong CHEN , Guobin LUO , Xinzhan PENG
IPC: C12N15/10 , C12Q1/6806 , C12Q1/6853 , C12Q1/6855
Abstract: Provided are methods for preparing a library of target nucleic acid sequences, as well as compositions and uses therefor. Methods comprise contacting a nucleic acid sample with a plurality of adaptors capable of amplification of one or more target nucleic acid sequences under conditions wherein the target nucleic acid(s) undergo a first amplification; digesting the resulting first amplification products; repairing the digested target amplicons; and amplifying the repaired products in a second amplification, thereby producing a library of target nucleic acid sequence. Each of the plurality of adaptor compositions comprise a handle and a targeted nucleic acid sequence and optionally one or more tag sequences. Provided methods may be carried out in a single, addition only workflow reaction, allowing for rapid production of highly multiplexed targeted libraries, optionally including unique tag sequences. Resulting library compositions are useful for a variety of applications, including sequencing applications.
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