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    Library preparation methods and compositions and uses therefor
    1.
    发明授权
    Library preparation methods and compositions and uses therefor 有权

    公开(公告)号：US11001836B2

    公开(公告)日：2021-05-11

    申请号：US16024175

    申请日：2018-06-29

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Mark Andersen ,  Daniel Mazur ,  Sihong Chen ,  Guobin Luo ,  Xinzhan Peng

    IPC: C12N15/10 ,  C12Q1/6806 ,  C12Q1/6853 ,  C12Q1/6855 ,  C12Q1/686 ,  C40B20/04 ,  C40B50/06 ,  C40B40/06

    Abstract: Provided are methods for preparing a library of target nucleic acid sequences, as well as compositions and uses therefor. Methods comprise contacting a nucleic acid sample with a plurality of adaptors capable of amplification of one or more target nucleic acid sequences under conditions wherein the target nucleic acid(s) undergo a first amplification; digesting the resulting first amplification products; repairing the digested target amplicons; and amplifying the repaired products in a second amplification, thereby producing a library of target nucleic acid sequence. Each of the plurality of adaptor compositions comprise a handle and a targeted nucleic acid sequence and optionally one or more tag sequences. Provided methods may be carried out in a single, addition only workflow reaction, allowing for rapid production of highly multiplexed targeted libraries, optionally including unique tag sequences. Resulting library compositions are useful for a variety of applications, including sequencing applications.

    Polymerase compositions and kits, and methods of using and making the same
    6.
    发明授权
    Polymerase compositions and kits, and methods of using and making the same 有权

    公开(公告)号：US11866740B2

    公开(公告)日：2024-01-09

    申请号：US17302621

    申请日：2021-05-07

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Daniel Mazur ,  Peter B. Vander Horn ,  Eileen Tozer ,  Sihong Chen ,  Guobin Luo ,  Joshua Shirley ,  Kevin Heinemann

    IPC: C12N9/12 ,  C12Q1/686

    CPC classification number: C12N9/1252 ,  C12Q1/686 ,  C12Y207/07007 ,  C12Q1/686 ,  C12Q2521/101

    Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, recombinant polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. In some aspects, the disclosure provides recombinant polymerases that yield lower systematic error rates and/or improved accuracy, when used in sequencing by synthesis reactions as compared to a control polymerase. In one aspect, the disclosure relates to recombinant polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In another aspect, the recombinant polymerases are useful for the amplification of nucleic acid templates during PCR, emPCR, isothermal amplification, recombinase polymerase amplification, rolling circle amplification, strand displacement amplification and proximity ligation amplification. In some aspects, the disclosure relates to recombinant polymerases useful for the generation of nucleic acid libraries and/or nucleic acid templates.

    Polymerase compositions, methods of making and using same
    9.
    发明授权
    Polymerase compositions, methods of making and using same 有权

    公开(公告)号：US09976128B2

    公开(公告)日：2018-05-22

    申请号：US14754203

    申请日：2015-06-29

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Peter Vander Horn ,  Theo Nikiforov ,  Guobin Luo ,  Mindy Landes ,  Daniel Mazur ,  Eileen Tozer ,  Tommie Lincecum

    IPC: C12N9/12 ,  C12Q1/68

    CPC classification number: C12N9/1252 ,  C12Q1/6846 ,  C12Q1/686 ,  C12Y207/07007

    Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragment thereof are provided that allow for nucleic acid amplification. In one aspect, the disclosure relates to modified polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In some aspects, the disclosure relates to modified polymerases useful for the generation of nucleic acid libraries or nucleic acid templates for use in various downstream processes. In some aspects, the disclosure relates to the identification of homologous amino acid mutations that can be transferred across classes or families of polymerases to provide novel polymerases with altered catalytic properties. In some aspects, the disclosure provides modified polymerases having enhanced catalytic properties as compared to a reference polymerase.

    LINKING METHODS, COMPOSITIONS, SYSTEMS, KITS AND APPARATUSES
    10.
    发明申请
    LINKING METHODS, COMPOSITIONS, SYSTEMS, KITS AND APPARATUSES 审中-公开

    公开(公告)号：US20180105805A1

    公开(公告)日：2018-04-19

    申请号：US15786223

    申请日：2017-10-17

    Applicant: Life Technologies Corporation

    Inventor： John DAVIDSON ,  Theo Nikiforov ,  Guobin Luo

    IPC: C12N11/10 ,  C12N9/12

    CPC classification number: C12N11/10 ,  C12N9/1241 ,  C12Q1/68 ,  G01N2333/9126

    Abstract: In some embodiments, the disclosure relates generally to methods as well as related compositions, systems, kits and apparatus comprising linking proteins to target compounds and/or to locations of interest using tethers. For example, the tether can be used to link the protein to a target compound, for example, to link an enzyme to a substrate. Similarly, the tether can be used to link the protein at or near a desired location on a surface. In one group of embodiments, the tether includes a polynucleotide and the target compound or location on the surface includes another polynucleotide that is capable of hybridizing to the tether. In such embodiments, the tether can be used to link the protein to the target compound or location using nucleic acid hybridization.

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