公开(公告)号：WO2013082518A1

公开(公告)日：2013-06-06

申请号：PCT/US2012/067427

申请日：2012-11-30

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： JONES, Meredith, Bauman ,  VASU, Sanjay ,  JUDD, David

IPC: B01D15/38 ,  G01N30/14

CPC classification number: G01N30/04 ,  B01D15/12 ,  B01D15/20 ,  B01D15/3828 ,  G01N2030/8831

Abstract: Compositions and methods for increasing protein recovery and/ or reducing metal ion stripping in IMAC chromatography or from resins used therein are provided. Samples like spent/ conditioned cell culture media or cell extracts containing native or recombinant proteins, were supplemented with metal ions before loading on to IMAC resins or columns to reduce metal ion stripping and/ or to increase protein recovery. The compositions and methods discussed herein work for a variety of metal affinity resins. Use of the compositions and methods disclosed may result in reusability of columns or resins, and improve the yield, purity and cost-effectiveness of using metal-ion based chromatography. The process is scalable to large-scale commercial levels.

Abstract translation: 提供了用于增加IMAC色谱中或从其中使用的树脂中的蛋白质回收和/或还原金属离子剥离的组合物和方法。 含有天然或重组蛋白质的废弃/调节细胞培养基或细胞提取物的样品在加载到IMAC树脂或柱之前补充金属离子以减少金属离子剥离和/或增加蛋白质回收。 本文讨论的组合物和方法适用于各种金属亲和树脂。 使用所公开的组合物和方法可以导致柱或树脂的可再利用性，并且提高使用基于金属离子的色谱法的产率，纯度和成本效益。 该过程可扩展到大规模的商业级别。

公开(公告)号：WO2013096858A1

公开(公告)日：2013-06-27

申请号：PCT/US2012/071411

申请日：2012-12-21

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： FIKE, Richard ,  BARRETT, Shawn ,  JIANG, Zhou ,  JUDD, David

IPC: C12N5/00

CPC classification number: C12N5/005 ,  B01J13/06 ,  B01J13/22 ,  B29C67/24 ,  B29L2009/00 ,  C12N5/0018 ,  C12N2500/30 ,  C12N2500/38 ,  C12N2511/00 ,  C12N2533/40 ,  C12N2533/74

Abstract: Compositions and methods are described for preparing media, feeds, and supplements. Such methods and medias may display increased stability of labile components and may use, for example, microsuspension and/or encapsulation technologies, chelation, and optionally, coating and/or mixing the labile compounds with anti-oxidants. The compositions may withstand thermal and/or irradiation treatment and have reduced virus number. These techniques may result in product with extended shelf-life, extended release of their internal components into culture, or in product that can be added aseptically into a bioreactor using minimal volumes. The compositions and methods may optimize the bioproduction workflow and increase efficiency.

Abstract translation: 描述了制备培养基，饲料和补品的组合物和方法。 这样的方法和介质可以显示不稳定组分的增加的稳定性，并且可以使用例如微悬浮和/或封装技术，螯合，以及任选地，将不稳定化合物与抗氧化剂包衣和/或混合。 组合物可以承受热和/或照射治疗并且具有减少的病毒数量。 这些技术可能导致产品具有延长的保质期，将其内部组分延长释放到培养物中，或可以无菌地添加到生物反应器中使用最小体积的产品。 组合物和方法可以优化生物生产工作流程并提高效率。

公开(公告)号：EP2794853B1

公开(公告)日：2020-04-22

申请号：EP12814100.9

申请日：2012-12-21

Applicant: Life Technologies Corporation

Inventor： FIKE, Richard ,  BARRETT, Shawn ,  JIANG, Zhou ,  JUDD, David

IPC: C12N5/00

公开(公告)号：EP3702442A1

公开(公告)日：2020-09-02

申请号：EP20170574.6

申请日：2012-12-21

Applicant: Life Technologies Corporation

Inventor： FIKE, Richard ,  BARRETT, Shawn ,  JIANG, Zhou ,  JUDD, David

IPC: C12N5/00

Abstract: Compositions and methods are described for preparing media, feeds, and supplements. Such methods and medias may display increased stability of labile components and may use, for example, microsuspension and/or encapsulation technologies, chelation, and optionally, coating and/or mixing the labile compounds with anti-oxidants. The compositions may withstand thermal and/or irradiation treatment and have reduced virus number. These techniques may result in product with extended shelf-life, extended release of their internal components into culture, or in product that can be added aseptically into a bioreactor using minimal volumes. The compositions and methods may optimize the bioproduction workflow and increase efficiency.

公开(公告)号：EP2794853A1

公开(公告)日：2014-10-29

申请号：EP12814100.9

申请日：2012-12-21

Applicant: Life Technologies Corporation

Inventor： FIKE, Richard ,  BARRETT, Shawn ,  JIANG, Zhou ,  JUDD, David

IPC: C12N5/00

Abstract: Compositions and methods are described for preparing media, feeds, and supplements. Such methods and medias may display increased stability of labile components and may use, for example, microsuspension and/or encapsulation technologies, chelation, and optionally, coating and/or mixing the labile compounds with anti-oxidants. The compositions may withstand thermal and/or irradiation treatment and have reduced virus number. These techniques may result in product with extended shelf-life, extended release of their internal components into culture, or in product that can be added aseptically into a bioreactor using minimal volumes. The compositions and methods may optimize the bioproduction workflow and increase efficiency.

Abstract translation: 描述了制备培养基，饲料和补品的成分和方法。 这样的方法和介质可以显示不稳定组分的增加的稳定性，并且可以使用例如微悬浮和/或包封技术，螯合和任选地，将不稳定化合物与抗氧化剂包衣和/或混合。 组合物可以承受热和/或照射治疗并且具有减少的病毒数量。 这些技术可能导致产品具有延长的保存期限，将其内部组分延长释放到培养物中，或可以无菌地添加到生物反应器中使用最小体积的产品。 组合物和方法可优化生物生产工作流程并提高效率。