公开(公告)号：WO2017069781A1

公开(公告)日：2017-04-27

申请号：PCT/US2015/057217

申请日：2015-10-23

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： MARTIN, Philip

IPC: C12N1/06 ,  C12N15/10 ,  C12M1/00 ,  B01D61/14

CPC classification number: C12N1/06 ,  B01D61/147 ,  C12M47/04 ,  C12M47/06 ,  C12N15/1017

Abstract: A cell lysis workflow involving a cell suspension that is passed through a filter in a first flow direction from a first side of the filter toward a second side thereof. The filter captures a plurality of cells on the first side. A lysis solution is passed through the filter in a second flow direction opposite the first direction, thereby dislodging the plurality of cells captured on the filter, and resuspending the plurality of cells in the lysis solution on the first side of the filter. The lysis solution lyses the plurality of cells to produce a cellular lysate. The cellular lysate is passed through the filter in the first flow direction.

Abstract translation: 涉及细胞悬液的细胞溶解工作流程，所述细胞悬液沿第一流动方向从滤器的第一侧朝向其第二侧穿过过滤器。 过滤器捕获第一侧上的多个细胞。 裂解溶液沿与第一方向相反的第二流动方向通过过滤器，由此移除捕集在过滤器上的多个细胞，并将多个细胞重新悬浮在过滤器的第一侧上的裂解溶液中。 裂解溶液裂解多个细胞以产生细胞裂解物。 细胞裂解物以第一流动方向通过过滤器。