公开(公告)号：US11998910B2

公开(公告)日：2024-06-04

申请号：US17412376

申请日：2021-08-26

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Mark Reed ,  Zheng (Derek) Xing ,  David Marran ,  Earl Zergiebel

IPC: B01L3/00 ,  C12Q1/6874

CPC classification number: B01L3/502715 ,  B01L3/502707 ,  C12Q1/6874 ,  B01L2200/026 ,  B01L2200/06 ,  B01L2300/0609 ,  B01L2300/0636 ,  B01L2300/0848

Abstract: In an example, a method for preparing a nucleotide solution includes flowing an aqueous solution from an initial solution storage of a sequencing instrument continuously through a container fluidically coupled to the sequencing instrument, the container comprising a nucleotide concentrate; and collecting the aqueous solution with nucleotide in a storage container.

公开(公告)号：US20240390901A1

公开(公告)日：2024-11-28

申请号：US18792286

申请日：2024-08-01

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jon Hoshizaki ,  Mark Reed ,  David Marran ,  Patrick Leung

IPC: B01L3/00 ,  A61J1/20

Abstract: A method of applying a fluid composition to a flow cell of a sensor device includes inserting the sensor device into a holder; pipetting an aliquot of the fluid composition into a pipette tip; and applying the aliquot of the fluid composition to an opening of a fluidic connector engaged with the flow cell of the sensor device. The fluidic connector includes a body; and a plurality of fluidics interfaces formed in the body, each fluidic interface of the plurality of fluidics interfaces includes an opening, a first port in fluid communication with the opening, a second port, and a third port in fluidic communication with the second port.

公开(公告)号：US20220176369A1

公开(公告)日：2022-06-09

申请号：US17526979

申请日：2021-11-15

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Joseph PODHASKY ,  Mark Reed ,  Nathan Kane

IPC: B01L3/02 ,  B01L3/00

Abstract: A system includes a pipetting system including a 3-axis gantry; a sled mechanism to select a magnetic comb from a set of magnetic combs; a fluorometer; and a set of receptacles to receive welled plates. A method for purifying nucleic acids includes applying a sample to a well of a multi-well plate, selecting a magnetic comb from a set of magnetic combs disposed on a gantry system, collecting magnetic beads using the magnetic comb, collecting nucleic acid using the magnetic beads, and eluting the nucleic acid from the beads.

公开(公告)号：US11117129B2

公开(公告)日：2021-09-14

申请号：US16543421

申请日：2019-08-16

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Mark Reed ,  Zheng (Derek) Xing ,  David Marran ,  Earl Zergiebel

IPC: B01L3/00 ,  C12Q1/6874

Abstract: In an example, a method for preparing a nucleotide solution includes flowing an aqueous solution from an initial solution storage of a sequencing instrument continuously through a container fluidically coupled to the sequencing instrument, the container comprising a nucleotide concentrate; and collecting the aqueous solution with nucleotide in a storage container.

公开(公告)号：US20240182965A1

公开(公告)日：2024-06-06

申请号：US18437474

申请日：2024-02-09

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Mark Reed ,  Chiu Tai Andrew Wong ,  David Marran

IPC: C12Q1/6874

CPC classification number: C12Q1/6874

Abstract: A sequencing system includes an automated sequencing instrument adapted to determine variant calls for one or more extracted polynucleotide samples with a performance of at least 98.5% raw read accuracy and a run time in a range of 5 hours to 14 hours to determine variant calls for 4 extracted polynucleotide samples using a targeted assay with one DNA pool per sample and an average amplicon size in a range of 100 to 120 bases.

公开(公告)号：US11905558B2

公开(公告)日：2024-02-20

申请号：US16994318

申请日：2020-08-14

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Mark Reed ,  Chiu Tai Andrew Wong ,  David Marran

IPC: C12Q1/6874

CPC classification number: C12Q1/6874

Abstract: A sequencing system includes an automated sequencing instrument adapted to determine variant calls for one or more extracted polynucleotide samples with a performance of at least 98.5% raw read accuracy and a run time in a range of 5 hours to 14 hours to determine variant calls for 4 extracted polynucleotide samples using a targeted assay with one DNA pool per sample and an average amplicon size in a range of 100 to 120 bases.

公开(公告)号：US11590499B2

公开(公告)日：2023-02-28

申请号：US16994289

申请日：2020-08-14

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jon Hoshizaki ,  Patrick Leung ,  David Marran ,  Mark Reed

IPC: B01L3/00

Abstract: An apparatus includes a fluidic coupler including an opening. A first port is in fluid communication with the opening and is to interface with an inlet of a flow cell of a sensor device. A second port is to interface with an outlet of the flow cell of the sensor device. A third port is in fluidic communication with the second port. The apparatus further includes a mechanical assembly moveable between a first position and a second position. The fluidic coupler is secured to the flow cell of the sensor device in the first position. The fluidic coupler is disengaged from the flow cell of the sensor device in the second position.

公开(公告)号：US12287307B2

公开(公告)日：2025-04-29

申请号：US16994202

申请日：2020-08-14

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Creighton Buie ,  Mark Reed ,  Albert Carrillo

IPC: G01N27/414

Abstract: A sensor device includes a substrate having a die attached to the substrate. The die includes an array of sensors and an array of wells cooperatively disposed over the array of sensors. The array of wells is exposed by the substrate. The sensor device further includes a flow cell secured to the substrate and defining a flow space disposed over the die and accessible to the array of wells. The flow cell defines a plurality of separate volumes. Each separate volume of the plurality of separate volumes has an inlet and an outlet. The plurality of separate volumes are separated by dividers. The dividers cover a set of wells of the array of wells.

公开(公告)号：US12076722B2

公开(公告)日：2024-09-03

申请号：US18107887

申请日：2023-02-09

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jon Hoshizaki ,  Patrick Leung ,  David Marran ,  Mark Reed

IPC: B01L3/00 ,  A61J1/20 ,  B01F23/00 ,  B01F23/41 ,  B01F101/23 ,  B23Q17/24 ,  C12M1/34 ,  C12Q1/04 ,  C12Q1/18 ,  C12Q1/686 ,  G01N21/3577 ,  G01N21/359 ,  G01N21/39 ,  G01N21/45 ,  G01N21/64 ,  G01N30/12 ,  G01N30/68 ,  G01N30/70 ,  G01N30/72 ,  G01N30/88 ,  G01N33/00 ,  G01N33/18 ,  G01N33/50 ,  G01N33/68

CPC classification number: B01L3/502738 ,  A61J1/2058 ,  B01L3/502715 ,  B01L3/50273 ,  B01L2300/0816

Abstract: A fluidic coupler to engage a plurality of flow cells of a sensor device includes a body and a plurality of fluidics interfaces formed in the body. Each fluidic interface of the plurality of fluidics interfaces includes an opening, a first port in fluid communication with the opening, a second port, and a third port in fluidic communication with the second port.

公开(公告)号：US20240307871A1

公开(公告)日：2024-09-19

申请号：US18675633

申请日：2024-05-28

Applicant: Life Technologies Corporation

Inventor： Mark Reed ,  David Marran ,  Earl Zergiebel ,  Zheng (Derek) Xing

IPC: B01L3/00 ,  C12Q1/6874

CPC classification number: B01L3/502715 ,  B01L3/502707 ,  C12Q1/6874 ,  B01L2200/026 ,  B01L2200/06 ,  B01L2300/0609 ,  B01L2300/0636 ,  B01L2300/0848

Abstract: A cartridge includes a housing having first and second major surfaces and defining a guide mechanism; and a plurality of containers disposed in the housing, each container of the plurality of containers having a receptacle and a lid, the lid formed of a clip and a seal, the clip to secure the seal to the receptacle, a frit disposed in the receptacle in an enclosed space defined by the receptacle and the seal, the seal having an inlet and an outlet in fluid communication with an enclosed space, the frit including a nucleotide concentrate.