公开(公告)号：US20220033858A1

公开(公告)日：2022-02-03

申请号：US17327002

申请日：2021-05-21

Applicant: LIFE TECHNOLOGIES CORPORATION ,  THERMO FISHER SCIENTIFIC GENEART GMBH

Inventor： Namritha RAVINDER ,  Korbinian HEIL ,  Yizhu GUO ,  Xiquan LIANG ,  Robert POTTER ,  Sanjay KUMAR

IPC: C12N15/90 ,  C12Q1/686 ,  C12N15/10 ,  C12N15/11 ,  C12P19/34

Abstract: The present disclosure generally relates to compositions and methods for the genetic modification of cells. In particular, the disclosure relates to CRISPR reagents and the use of such reagents.

公开(公告)号：US20180195089A1

公开(公告)日：2018-07-12

申请号：US15842664

申请日：2017-12-14

Applicant: LIFE TECHNOLOGIES CORPORATION ,  THERMO FISHER SCIENTIFIC GENEART GMBH

Inventor： Namritha RAVINDER ,  Korbinian HEIL ,  Yizhu GUO ,  Xiquan LIANG ,  Robert POTTER ,  Sanjay KUMAR

IPC: C12N15/90 ,  C12N15/10 ,  C12Q1/686 ,  C12P19/34 ,  C12N15/11

CPC classification number: C12N15/907 ,  C12N15/102 ,  C12N15/11 ,  C12N2310/3519 ,  C12P19/34 ,  C12Q1/686 ,  C12Q2525/143

Abstract: The present disclosure generally relates to compositions and methods for the genetic modification of cells. In particular, the disclosure relates to CRISPR reagents and the use of such reagents.

公开(公告)号：US20180010105A1

公开(公告)日：2018-01-11

申请号：US15607247

申请日：2017-05-26

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jeffrey ROGERS ,  Robert POTTER

IPC: C12N9/12 ,  C12P19/34 ,  C12N15/10

CPC classification number: C12N9/1276 ,  C12N15/1096 ,  C12P19/34 ,  C12Y207/07049

Abstract: The invention provides novel reverse transcriptases (RTs) with desirable properties such as increased thermostability, increased thermoreactivity and/or increased resistance to inhibitors. In certain embodiments, the invention provides methods of producing, amplifying and/or sequencing nucleic acid molecules (particularly cDNA molecules) using kits, compositions and/or reactions mixtures containing such novel reverse transcriptase enzymes.

公开(公告)号：US20230151345A1

公开(公告)日：2023-05-18

申请号：US18071206

申请日：2022-11-29

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Robert POTTER ,  Jonathan CHESNUT ,  Xiquan LIANG

IPC: C12N9/22 ,  C12N15/11 ,  C12N15/90

CPC classification number: C12N9/22 ,  C12N15/11 ,  C12N15/907 ,  C12N2310/20 ,  C12N2800/80

Abstract: The present disclosure generally relates to compositions and methods for improving the efficiency of homologous recombination. In particular, the disclosure relates to reagents and the use of such reagents.

公开(公告)号：US20240025939A1

公开(公告)日：2024-01-25

申请号：US17909091

申请日：2021-03-05

Applicant: LIFE TECHNOLOGIES CORPORATION ,  THERMO FISHER SCIENTIFIC GENEART GMBH

Inventor： Robert POTTER ,  Nikolai NETUSCHIL

IPC: C07H21/00 ,  C12Q1/686 ,  C12N9/22

CPC classification number: C07H21/00 ,  C12Q1/686 ,  C12N9/22

Abstract: The present disclosure generally relates to compositions and methods for the synthesis of nucleic acid molecules with low error rates. Provided, as examples, are compositions and methods for high throughput synthesis and assembly of nucleic acid molecules, in many instances, with high sequence fidelity. In many instances, thermostable mismatch recognition proteins (e.g., thermostable mismatch binding protein, thermostable mismatch endonucleases) will be present in compositions and use methods provided.

公开(公告)号：US20170306306A1

公开(公告)日：2017-10-26

申请号：US15520533

申请日：2015-10-26

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Robert POTTER ,  Jonathan CHESNUT ,  Xiquan LIANG

IPC: C12N9/22 ,  C12N15/90 ,  C12N2310/20

CPC classification number: C12N9/22 ,  C07K2319/00 ,  C07K2319/81 ,  C12N15/902 ,  C12N2310/20

Abstract: The present disclosure generally relates to compositions and methods for improving the efficiency of homologous recombination. In particular, the disclosure relates to reagents and the use of such reagents.

公开(公告)号：US20230242922A1

公开(公告)日：2023-08-03

申请号：US17931858

申请日：2022-09-13

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jonathan CHESNUT ,  Alicia IHLAN ,  Robert POTTER ,  Youngzee SONG

IPC: C12N15/62 ,  C12N15/90 ,  C12N9/22

CPC classification number: C12N15/62 ,  C12N15/907 ,  C12N9/22 ,  C07K2319/81

Abstract: The present disclosure relates to recombinant nucleases, recombinant nucleases operatively linked to nucleic acid binding domains, and methods of making and using them.

公开(公告)号：US20220372522A1

公开(公告)日：2022-11-24

申请号：US17766998

申请日：2020-10-07

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Xiquan LIANG ,  Pei-Zhong TANG ,  Robert POTTER

IPC: C12N15/90 ,  C12N9/22 ,  C12N15/11 ,  C07K14/245

Abstract: The present disclosure relates, in part, to improved methods of making single-stranded DNA (ssDNA) from double-stranded DNA (dsDNA), as well as use of the resulting ssDNA for genome engineering. The disclosure also relates, in part, to improved methods of genetic modification using single stranded DNA binding proteins.

公开(公告)号：US20220002716A1

公开(公告)日：2022-01-06

申请号：US17364956

申请日：2021-07-01

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Anilkumar KORE ,  Senthilvelan ANNAMALAI ,  Shanmugasundaram MUTHIAN ,  Robert POTTER ,  Tyson VONDERFECHT

IPC: C12N15/11 ,  C07H21/00 ,  C12N9/12 ,  A61K31/7088 ,  A61K47/54 ,  A61K38/45

Abstract: This specification generally relates to trinucleotide RNA cap analogs, methods of use thereof, and kits comprising same. In particular, the trinucleotide cap analogs provided herein permit ready detection and/or isolation of capped RNA transcripts in vitro and translation of capped mRNAs in vivo.

公开(公告)号：US20200080077A1

公开(公告)日：2020-03-12

申请号：US16536983

申请日：2019-08-09

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Xiquan LIANG ,  Robert POTTER ,  Namritha RAVINDER ,  Shantanu KUMAR

IPC: C12N15/10 ,  C12N15/113 ,  C12N15/90

Abstract: The present disclosure generally relates to compositions and methods for improving the efficiency of homologous recombination. In particular, the disclosure relates to reagents and the use of such reagents.