公开(公告)号：US10570250B2

公开(公告)日：2020-02-25

申请号：US16107818

申请日：2018-08-21

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Kyle Gee ,  Xin Wang ,  Hee Chol Kang ,  Aimei Chen ,  Yexin Wu ,  Yi-Zhen Hu ,  Robert Aggeler ,  Quentin Low

IPC: C08G61/12 ,  H01L51/00 ,  C07F7/08 ,  C08L65/00 ,  G01N33/533 ,  G01N33/58 ,  C09K11/06 ,  G01N33/545

Abstract: Water-soluble, conjugated polymers containing one or more dibenzosilole monomer residues, as well as compositions, kits, and methods of making and using such polymers are disclosed. Also disclosed are dibenzosilole derivatives substituted with one or more water-solubilizing groups, and methods of making and using such derivatives to prepare water-soluble dibenzosilole polymers.

公开(公告)号：US20180355098A1

公开(公告)日：2018-12-13

申请号：US16107818

申请日：2018-08-21

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Kyle Gee ,  Xin Wang ,  Hee Chol Kang ,  Aimei Chen ,  Yexin Wu ,  Yi-Zhen Hu ,  Robert Aggeler ,  Quentin Low

IPC: C08G61/12 ,  H01L51/00 ,  G01N33/545 ,  C09K11/06 ,  G01N33/58 ,  G01N33/533 ,  C08L65/00 ,  C07F7/08

CPC classification number: C08G61/123 ,  C07F7/0816 ,  C07F7/083 ,  C08G2261/122 ,  C08G2261/124 ,  C08G2261/1424 ,  C08G2261/145 ,  C08G2261/1644 ,  C08G2261/1646 ,  C08G2261/3142 ,  C08G2261/3244 ,  C08G2261/334 ,  C08G2261/344 ,  C08G2261/411 ,  C08G2261/522 ,  C08G2261/5222 ,  C08G2261/72 ,  C08G2261/94 ,  C08L65/00 ,  C09K11/06 ,  C09K2211/1416 ,  C09K2211/1491 ,  G01N33/533 ,  G01N33/545 ,  G01N33/582 ,  H01L51/0039 ,  H01L51/0043 ,  H01L51/0094

Abstract: Water-soluble, conjugated polymers containing one or more dibenzosilole monomer residues, as well as compositions, kits, and methods of making and using such polymers are disclosed. Also disclosed are dibenzosilole derivatives substituted with one or more water-solubilizing groups, and methods of making and using such derivatives to prepare water-soluble dibenzosilole polymers.

公开(公告)号：US11768156B2

公开(公告)日：2023-09-26

申请号：US17936947

申请日：2022-09-30

Applicant: Life Technologies Corporation

Inventor： Kyle Gee ,  Kathleen Kihn ,  Yi-Zhen Hu ,  Yexin Wu ,  Kathleen Free

IPC: G01N21/64 ,  C07D311/82 ,  C07D209/10 ,  C07F15/00 ,  C07F9/24

CPC classification number: G01N21/6486 ,  C07D209/10 ,  C07D311/82 ,  C07F9/2466 ,  C07F15/0093 ,  G01N21/6428

Abstract: Provided herein are compounds, compositions, kits, uses, and methods for assessing the viability of cells and/or quantifying the amount of live and dead cells in a mixture using differential stains. In some embodiments, the compounds are used in culture media or can function independently of fixation and/or permeabilization. In some embodiments, the compounds comprise a platinum atom. In some embodiments, the compounds comprise a nitrogen mustard moiety.

公开(公告)号：US20190195801A1

公开(公告)日：2019-06-27

申请号：US16205702

申请日：2018-11-30

Applicant: Life Technologies Corporation

Inventor： Kyle Gee ,  Kathleen Kihn ,  Yi-Zhen Hu ,  Yexin Wu ,  Kathleen Free

IPC: G01N21/64 ,  C07F15/00 ,  C07D209/10 ,  C07D311/82

CPC classification number: G01N21/6486 ,  C07D209/10 ,  C07D311/82 ,  C07F15/0093 ,  G01N21/6428

Abstract: Provided herein are compounds, compositions, kits, uses, and methods for assessing the viability of cells and/or quantifying the amount of live and dead cells in a mixture using differential stains. In some embodiments, the compounds are used in culture media or can function independently of fixation and/or permeabilization. In some embodiments, the compounds comprise a platinum atom. In some embodiments, the compounds comprise a nitrogen mustard moiety.

公开(公告)号：US10087280B2

公开(公告)日：2018-10-02

申请号：US15491650

申请日：2015-08-14

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Kyle Gee ,  Xin Wang ,  Hee Chol Kang ,  Aimei Chen ,  Yexin Wu ,  Yi-Zhen Hu ,  Robert Aggeler ,  Quentin Low

IPC: C08G61/12 ,  C07F7/08 ,  C08L65/00 ,  G01N33/533 ,  G01N33/58 ,  H01L51/00 ,  C09K11/06 ,  G01N33/545

Abstract: Water-soluble, conjugated polymers containing one or more dibenzosilole monomer residues, as well as compositions, kits, and methods of making and using such polymers are disclosed. Also disclosed are dibenzosilole derivatives substituted with one or more water-solubilizing groups, and methods of making and using such derivatives to prepare water-soluble dibenzosilole polymers.

公开(公告)号：US09624424B2

公开(公告)日：2017-04-18

申请号：US14566166

申请日：2014-12-10

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Yi-Zhen Hu ,  Hee Chol Kang

IPC: C07D403/06 ,  C07D413/06 ,  C07D417/06 ,  C09K11/06 ,  C07D209/14 ,  C07D401/14 ,  C09B23/01 ,  C09B23/08 ,  G01N33/52 ,  C07D209/26 ,  C07D401/10 ,  C07H19/207

CPC classification number: C07H19/207 ,  C07D209/14 ,  C07D209/26 ,  C07D401/10 ,  C07D401/14 ,  C09B23/0041 ,  C09B23/0066 ,  C09B23/086 ,  C09B23/164 ,  C09K11/06 ,  C09K2211/1007 ,  C09K2211/1014 ,  C09K2211/1022 ,  C09K2211/1044 ,  C09K2211/1059 ,  C09K2211/1074 ,  G01N33/52

Abstract: Provided herein are heptamethine cyanine dyes having a large Stokes shift, and the salts and conjugates thereof. Also provided are methods of using and making such large Stokes shift dyes as fluorescence resonance energy transfer (FRET) acceptors or donors.

公开(公告)号：US11952495B1

公开(公告)日：2024-04-09

申请号：US17498377

申请日：2021-10-11

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Yi-Zhen Hu ,  Aimei Chen ,  Daniel Beacham ,  Chrisgen Vonnegut ,  Krishnamurthy Nacharaju

IPC: C09B11/24 ,  G01N21/64

CPC classification number: C09B11/24 ,  G01N21/6428 ,  G01N2021/6439

Abstract: The present disclosure provides for compounds of Formula (I),




its corresponding compounds of Formula (II)





or salts thereof and their use as fluorogenic pH sensors.

公开(公告)号：US20150079622A1

公开(公告)日：2015-03-19

申请号：US14384843

申请日：2013-02-26

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Yi-Zhen Hu ,  Aimei Chen ,  Hee Chol Kang ,  Kyle Gee ,  Bhaskar Mandavilli

IPC: G01N33/52 ,  C07D417/06

CPC classification number: G01N33/84 ,  C07D417/06 ,  C09B23/02 ,  C09B23/04 ,  C09B23/06 ,  G01N33/52

Abstract: Disclosed herein are compounds, compositions, methods, and kits for detecting reactive oxygen species (ROS) by conventional fluorescence microscopy, fluorescence spectroscopy, flow cytometry, and/or high content imaging. The compounds disclosed herein are novel reduced nucleic acid binding cyanine dyes, which dyes are probes for detecting ROS and measuring oxidative stress in cells either in vitro and/or in vivo. Also described herein are processes for preparing novel reduced dyes, i.e., ROS probes, for use in the disclosed compositions, methods and kits.

Abstract translation: 本文公开了通过常规荧光显微镜，荧光光谱，流式细胞术和/或高含量成像检测活性氧（ROS）的化合物，组合物，方法和试剂盒。 本文公开的化合物是新的还原性核酸结合花青染料，其染料是用于检测ROS的探针，并测定体外和/或体内细胞中的氧化应激。 本文还描述了用于制备用于所公开的组合物，方法和试剂盒的新型还原染料即ROS探针的方法。

公开(公告)号：US12258477B2

公开(公告)日：2025-03-25

申请号：US17150247

申请日：2019-08-08

Applicant: Life Technologies Corporation

Inventor： Jongtae Yang ,  Xin Wang ,  Yi-Zhen Hu ,  Kyle Gee ,  Aimei Chen ,  Aleksey Rukavishnikov

IPC: C09B11/28 ,  C07F7/08 ,  C07F9/6561 ,  C12Q1/6816 ,  C12Q1/6869 ,  G01N33/58

Abstract: Silicon-substituted rhodamine compounds are disclosed herein. Also described herein are SiR dyes comprising at least one vinyl group attached to the Si atom (10 position) of the SiR dye. Derivatives, functionalized versions, conjugates, kits, related synthetic methods and uses of SiR compounds also are provided. Silicon-rhodamine (SiR) dyes can provide bright fluorescence at far red wavelengths and exhibit good photostability. The compounds described herein can be useful for fluorescent labeling and detection of biological samples.

公开(公告)号：US11493446B2

公开(公告)日：2022-11-08

申请号：US16205702

申请日：2018-11-30

Applicant: Life Technologies Corporation

Inventor： Kyle Gee ,  Kathleen Kihn ,  Yi-Zhen Hu ,  Yexin Wu ,  Kathleen Free

IPC: G01N21/64 ,  C07D311/82 ,  C07D209/10 ,  C07F15/00 ,  C07F9/24

Abstract: Provided herein are compounds, compositions, kits, uses, and methods for assessing the viability of cells and/or quantifying the amount of live and dead cells in a mixture using differential stains. In some embodiments, the compounds are used in culture media or can function independently of fixation and/or permeabilization. In some embodiments, the compounds comprise a platinum atom. In some embodiments, the compounds comprise a nitrogen mustard moiety.