公开(公告)号：DE60109581T2

公开(公告)日：2006-01-19

申请号：DE60109581

申请日：2001-01-25

Applicant: LOMA LINDA UNIVERSITY LOMA LIN

Inventor： SHABAHANG SHAHROKH ,  SZALAY A ,  YU YONG

IPC: A61K6/00 ,  C12N1/20 ,  C12N1/21 ,  C12Q1/02 ,  C12Q1/04 ,  C12Q1/18 ,  C12Q1/22 ,  G01N33/50

Abstract: A method for the evaluation of a material to determine whether the material is susceptible to bacterial contamination or colonization comprising providing bacteria which are modified to produce a first detectable signal, exposing the material being evaluated to the bacteria and determining whether the first signal is present determining whether the first signal is present on the material or within the material.

公开(公告)号：DE69726306T2

公开(公告)日：2004-10-14

申请号：DE69726306

申请日：1997-09-24

Applicant: LOMA LINDA UNIVERSITY LOMA LIN

Inventor： SZALAY A ,  WANG GEFU ,  WANG YUBAO

IPC: C12N15/09 ,  C07K14/00 ,  C07K14/435 ,  C07K16/00 ,  C07K16/40 ,  C07K19/00 ,  C12N9/02 ,  C12N15/02 ,  C12P21/04 ,  C12P21/08 ,  C12P21/06 ,  C12N1/20 ,  C07H21/04

Abstract: A fusion gene is provided comprising the cDNA of Renilla luciferase and the cDNA of the "humanized" Aequorea green fluorescent protein. The fusion gene was used to produce a novel protein, the "Renilla-GFP fusion protein," which displayed both the luciferase activity of Renilla luciferase, and the green fluorescence of GFP. The Renilla-GFP fusion gene is useful as a double marker for monitoring gene expression quantitatively in UV light and by enzyme activity.

公开(公告)号：DE69726306D1

公开(公告)日：2003-12-24

申请号：DE69726306

申请日：1997-09-24

Applicant: LOMA LINDA UNIVERSITY LOMA LIN

Inventor： SZALAY A ,  WANG GEFU ,  WANG YUBAO

IPC: C12N15/09 ,  C07K14/00 ,  C07K14/435 ,  C07K16/00 ,  C07K16/40 ,  C07K19/00 ,  C12N9/02 ,  C12N15/02 ,  C12P21/04 ,  C12P21/08 ,  C12P21/06 ,  C12N1/20 ,  C07H21/04

Abstract: A fusion gene is provided comprising the cDNA of Renilla luciferase and the cDNA of the "humanized" Aequorea green fluorescent protein. The fusion gene was used to produce a novel protein, the "Renilla-GFP fusion protein," which displayed both the luciferase activity of Renilla luciferase, and the green fluorescence of GFP. The Renilla-GFP fusion gene is useful as a double marker for monitoring gene expression quantitatively in UV light and by enzyme activity.

公开(公告)号：DE60109581D1

公开(公告)日：2005-04-28

申请号：DE60109581

申请日：2001-01-25

Applicant: LOMA LINDA UNIVERSITY LOMA LIN

Inventor： SHABAHANG SHAHROKH ,  SZALAY A ,  YU YONG

IPC: A61K6/00 ,  C12N1/21 ,  C12Q1/02 ,  C12Q1/04 ,  C12Q1/18 ,  G01N33/50 ,  C12Q1/22 ,  C12N1/20

Abstract: A method for the evaluation of a material to determine whether the material is susceptible to bacterial contamination or colonization comprising providing bacteria which are modified to produce a first detectable signal, exposing the material being evaluated to the bacteria and determining whether the first signal is present determining whether the first signal is present on the material or within the material.