公开(公告)号：DE69726306T2

公开(公告)日：2004-10-14

申请号：DE69726306

申请日：1997-09-24

Applicant: LOMA LINDA UNIVERSITY LOMA LIN

Inventor： SZALAY A ,  WANG GEFU ,  WANG YUBAO

IPC: C12N15/09 ,  C07K14/00 ,  C07K14/435 ,  C07K16/00 ,  C07K16/40 ,  C07K19/00 ,  C12N9/02 ,  C12N15/02 ,  C12P21/04 ,  C12P21/08 ,  C12P21/06 ,  C12N1/20 ,  C07H21/04

Abstract: A fusion gene is provided comprising the cDNA of Renilla luciferase and the cDNA of the "humanized" Aequorea green fluorescent protein. The fusion gene was used to produce a novel protein, the "Renilla-GFP fusion protein," which displayed both the luciferase activity of Renilla luciferase, and the green fluorescence of GFP. The Renilla-GFP fusion gene is useful as a double marker for monitoring gene expression quantitatively in UV light and by enzyme activity.

公开(公告)号：DE69726306D1

公开(公告)日：2003-12-24

申请号：DE69726306

申请日：1997-09-24

Applicant: LOMA LINDA UNIVERSITY LOMA LIN

Inventor： SZALAY A ,  WANG GEFU ,  WANG YUBAO

IPC: C12N15/09 ,  C07K14/00 ,  C07K14/435 ,  C07K16/00 ,  C07K16/40 ,  C07K19/00 ,  C12N9/02 ,  C12N15/02 ,  C12P21/04 ,  C12P21/08 ,  C12P21/06 ,  C12N1/20 ,  C07H21/04

Abstract: A fusion gene is provided comprising the cDNA of Renilla luciferase and the cDNA of the "humanized" Aequorea green fluorescent protein. The fusion gene was used to produce a novel protein, the "Renilla-GFP fusion protein," which displayed both the luciferase activity of Renilla luciferase, and the green fluorescence of GFP. The Renilla-GFP fusion gene is useful as a double marker for monitoring gene expression quantitatively in UV light and by enzyme activity.