公开(公告)号：US10386377B2

公开(公告)日：2019-08-20

申请号：US14889366

申请日：2014-05-07

Applicant: Micronics, Inc.

Inventor： Andrew W. Kolb ,  Carolina L. Elmufdi ,  C. Frederick Battrell

IPC: B01L3/00 ,  G01N33/80 ,  G01N33/49

Abstract: Microfluidic cartridges or devices for serum separation and blood cross-match analysis are provided. The devices may include a serum separation subcircuit alone or in combination with a solute mixing subcircuit. The serum separation subcircuit promotes on-cartridge clotting of a blood sample and manipulates the flow of the separated serum sample for subsequent cross-match analysis with a second blood sample, for example. The solute mixing subcircuit includes at least two intake channels, one for a whole blood sample from, for example, a blood donor and the other for the separated serum sample from, for example, a transfusion recipient. The solute mixing subcircuit further includes a serpentine mixing channel conjoined to a downstream channel. Under vacuum generated by a conjoined finger pump, the two input streams fill the serpentine mixing and downstream channels due to capillary action, enabling visualization of an agglutination reaction.

公开(公告)号：US20160109467A1

公开(公告)日：2016-04-21

申请号：US14889366

申请日：2014-05-07

Applicant: MICRONICS, INC.

Inventor： Andrew W. Kolb ,  Carolina L. Elmufdi ,  C. Frederick Battrell

IPC: G01N33/80 ,  B01L3/00

CPC classification number: G01N33/80 ,  B01L3/502715 ,  B01L3/502723 ,  B01L3/50273 ,  B01L3/502738 ,  B01L3/502753 ,  B01L2200/027 ,  B01L2200/10 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0883 ,  B01L2400/0406 ,  B01L2400/0481 ,  G01N33/491

Abstract: Microfluidic cartridges or devices for serum separation and blood cross-match analysis are provided. The devices may include a serum separation subcircuit alone or in combination with a solute mixing subcircuit. The serum separation subcircuit promotes on-cartridge clotting of a blood sample and manipulates the flow of the separated serum sample for subsequent cross-match analysis with a second blood sample, for example. The solute mixing subcircuit includes at least two intake channels, one for a whole blood sample from, for example, a blood donor and the other for the separated serum sample from, for example, a transfusion recipient. The solute mixing subcircuit further includes a serpentine mixing channel conjoined to a downstream channel. Under vacuum generated by a conjoined finger pump, the two input streams fill the serpentine mixing and downstream channels due to capillary action, enabling visualization of an agglutination reaction.

Abstract translation: 提供用于血清分离和血液交叉匹配分析的微流控盒或装置。 装置可以包括单独的血清分离子电路或者与溶质混合分支电路组合。 血清分离辅助电路促进血液样品的盒式凝血，并操纵分离的血清样品的流动，用于随后与第二血液样品的交叉匹配分析。 溶质混合分支电路包括至少两个进气通道，一个用于来自例如血液供体的全血样品，另一个用于来自例如输血接受者的分离的血清样品。 溶质混合分支电路还包括与下游通道结合的蛇形混合通道。 在由结合的手指泵产生的真空下，两个输入流由于毛细管作用而填充蛇形混合和下游通道，从而使凝集反应可视化。