公开(公告)号：WO1995028476A1

公开(公告)日：1995-10-26

申请号：PCT/US1995004765

申请日：1995-04-17

Applicant: MIDWEST RESEARCH INSTITUTE

Inventor： MIDWEST RESEARCH INSTITUTE ,  PICATAGGIO, Stephen, K. ,  ZHANG, Min ,  EDDY, Christina, K. ,  DEANDA, Kristine, A. ,  FINKELSTEIN, Mark ,  MOHAGHEGHI, Ali ,  NEWMAN, Mildred, M. ,  McMILLAN, James, D.

IPC: C12N01/21

CPC classification number: C12N9/10 ,  C12N9/90 ,  C12N15/52 ,  C12P7/065 ,  Y02E50/17

Abstract: The invention relates to microorganisms which normally do not ferment pentose sugar and which are genetically altered to ferment pentose sugar to produce ethanol, and fermentation processes utilizing the same. Examples include Zymomonas mobilis which has been transformed with combinations of E. coli genes for xylose isomerase, xylulokinase, transaldolase, transketolase, L-arabinose isomerase, L-ribulokinase, and L-ribulose 5-phosphate 4-epimerase. Expression of the added genes are under the control of Zymomonas mobilis promoters. These newly created microorganisms are useful for fermenting pentoses and glucose, produced by hydrolysis of hemicellulose and cellulose, to produce ethanol.

Abstract translation: 本发明涉及通常不会发酵戊糖并且遗传改变以发酵戊糖以产生乙醇的微生物，以及利用其的发酵方法。 实例包括已经用木糖异构酶，木酮糖激酶，转醛醇酶，转酮酶，L-阿拉伯糖异构酶，L-核糖激酶和L-核酮糖5-磷酸4-差向异构酶的大肠杆菌基因的组合转化的运动发酵单胞菌（Zymomonas mobilis）。 添加的基因的表达在运动发酵单胞菌启动子的控制下。 这些新产生的微生物可用于发酵通过半纤维素和纤维素的水解产生的戊糖和葡萄糖以产生乙醇。

公开(公告)号：WO1997013842A1

公开(公告)日：1997-04-17

申请号：PCT/US1996015442

申请日：1996-09-27

Applicant: MIDWEST RESEARCH INSTITUTE

Inventor： MIDWEST RESEARCH INSTITUTE ,  PICATAGGIO, Stephen, K. ,  ZHANG, Min ,  FRANDEN, Mary, Ann ,  MC MILLAN, James, D. ,  FINKELSTEIN, Mark

IPC: C12N01/20

CPC classification number: C12N9/1205 ,  C12N9/90 ,  C12N15/746 ,  C12P7/56

Abstract: A recombinant Lactobacillus MONT4 is provided which has been genetically engineered with xylose isomerase and xylulokinase genes from Lactobacillus pentosus to impart to the Lactobacillus MONT4 the ability to ferment lignocellulosic biomass containining xylose to lactic acid.

Abstract translation: 提供了重组乳酸杆菌MONT4，其已经用来自戊糖乳杆菌的木糖异构酶和木酮糖激酶基因进行了遗传工程改造以赋予乳酸菌MONT4以发酵木糖纤维素生物质将木糖包含在乳酸中的能力。

公开(公告)号：EP1005530A1

公开(公告)日：2000-06-07

申请号：EP98918955.0

申请日：1998-04-05

Applicant: Midwest Research Institute

Inventor： ZHANG, Min ,  CHOU, Yat-Chen ,  PICATAGGIO, Stephen, K. ,  FINKELSTEIN, Mark

IPC: C12N1/21 ,  C12N15/74

CPC classification number: C12N9/10 ,  C12N9/90 ,  C12N15/52 ,  C12P7/065 ,  C12R1/01 ,  Y02E50/17 ,  Y10S435/822

Abstract: A microorganism of the genus Zymomonas containing exogenous genes encoding xylose isomerase, xylulokinase, L-arabinose isomerase, L-ribulokinase, L-ribulose-5-phosphate 4-epimerase, transaldolase and transketolase and further comprising at least one promotor recognized by Zymomonas which regulates the expression of at least one of the genes, wherein the microorganism is capable of growing on arabinose and xylose as carbon sources and fermenting the arabinose and xylose to ethanol in about 75 % theoretical yield and, wherein the microorganism without the genes is incapable of growing on or fermenting the arabinose and xylose to ethanol.

公开(公告)号：EP1005530B1

公开(公告)日：2006-03-15

申请号：EP98918955.0

申请日：1998-04-05

Applicant: Midwest Research Institute

Inventor： ZHANG, Min ,  CHOU, Yat-Chen ,  PICATAGGIO, Stephen, K. ,  FINKELSTEIN, Mark

IPC: C12N1/21 ,  C12N15/74 ,  C12N15/52 ,  C12P7/06 ,  C12N15/70

CPC classification number: C12N9/10 ,  C12N9/90 ,  C12N15/52 ,  C12P7/065 ,  C12R1/01 ,  Y02E50/17 ,  Y10S435/822

Abstract: A microorganism of the genus Zymomonas containing exogenous genes encoding xylose isomerase, xylulokinase, L-arabinose isomerase, L-ribulokinase, L-ribulose-5-phosphate 4-epimerase, transaldolase and transketolase and further comprising at least one promotor recognized by Zymomonas which regulates the expression of at least one of the genes, wherein the microorganism is capable of growing on arabinose and xylose as carbon sources and fermenting the arabinose and xylose to ethanol in about 75 % theoretical yield and, wherein the microorganism without the genes is incapable of growing on or fermenting the arabinose and xylose to ethanol.

公开(公告)号：EP0859829A1

公开(公告)日：1998-08-26

申请号：EP96935981.0

申请日：1996-09-27

Applicant: Midwest Research Institute

Inventor： PICATAGGIO, Stephen, K. ,  ZHANG, Min ,  FRANDEN, Mary, Ann ,  MC MILLAN, James, D. ,  FINKELSTEIN, Mark

IPC: C12N9 ,  C12N15 ,  C12P7

CPC classification number: C12N9/1205 ,  C12N9/90 ,  C12N15/746 ,  C12P7/56

Abstract: A recombinant Lactobacillus MONT4 is provided which has been genetically engineered with xylose isomerase and xylulokinase genes from Lactobacillus pentosus to impart to the Lactobacillus MONT4 the ability to ferment lignocellulosic biomass containining xylose to lactic acid.