公开(公告)号：CA1155838A

公开(公告)日：1983-10-25

申请号：CA414642

申请日：1982-11-01

Applicant: MILES LAB

Inventor： CARRICO ROBERT J ,  NGO THAT T

IPC: C07H15/26

Abstract: A .beta.-galactosyl-umbelliferone-labeled conjugate of the formula: wherein -(NH)L is a protein or polypeptide, such as an immunoglobulin, bound through an amino group thereof, n is an integer from 2 through 10, m is an integer from 1 through 10, and p is on the average from 1 to the number of available amino groups in L. An intermediate in the synthesis of such labeled conjugate is also provided. The labeled conjugates are useful as reagents in specific binding assays (e.g., immunoassays) for determining the conjugated protein or polypeptide, or a specific binding analog or partner thereof, in liquid media.

公开(公告)号：CA1152491A

公开(公告)日：1983-08-23

申请号：CA359240

申请日：1980-08-28

Applicant: MILES LAB

Inventor： CARRICO ROBERT J ,  NGO THAT T

IPC: G01N33/532 ,  A61K39/00 ,  C07H19/20 ,  C07H19/207 ,  C07H21/00 ,  C07K14/00 ,  C12N9/04 ,  G01N33/533 ,  G01N33/535 ,  C07C103/52 ,  C07G7/00

Abstract: Flavin adenine dinucleotide-labeled conjugates of the formula: wherein Ribose represents the riboflavin -pyrophosphate-ribose residue in flavin adenine dinucleotide (FAD), ?NH)L is a protein or polypeptide (e.g., an immunoglobulin) bound through an amino group thereof, n is an integer from 2 through 10, ? is an integer from 1 through 10, and p is on the average from 1 to the number of available amino groups in L. The FAD-labeled conjugates are useful as reagents in specific binding assays (e.g., immunoasssys) to determine the conjugated protein or polypeptide, or a specific binding analog or partner thereof, in liquid medii.

公开(公告)号：CA1152490A

公开(公告)日：1983-08-23

申请号：CA359111

申请日：1980-08-27

Applicant: MILES LAB

Inventor： CARRICO ROBERT J ,  NGO THAT T

IPC: G01N33/532 ,  A61K39/395 ,  C07H17/075 ,  C07H21/00 ,  C07K14/00 ,  G01N33/533 ,  C07C103/52 ,  C07G7/00

Abstract: A .beta.-galactosyl-umbelliferone-labeled conjugate of the formula: wherein ?NH)L is a protein or polypeptide, such as an immunoglobulin, bound through an amino group thereof, n is an integer from 2 through 10, m is an integer from 1 through 10, and ? is on the average from 1 to the number of available amino groups in L. An intermediate in the synthesis of such labeled conjugate is also provided. The libeled conjugates are useful as reagents in specific binding assays (e.g., immunoassays) for determining the conjugated protein or polypeptide, or a specific binding analog or partner thereof, in liquid media.