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    HYBRIDIZATION ASSAY WITH IMMOBILIZATION OF HYBRIDS BY ANTI-HYBRID BINDING
    2.
    发明专利
    HYBRIDIZATION ASSAY WITH IMMOBILIZATION OF HYBRIDS BY ANTI-HYBRID BINDING 未知

    公开(公告)号：CA1250232A

    公开(公告)日：1989-02-21

    申请号：CA469907

    申请日：1984-12-12

    Applicant: MILES LAB

    Inventor： CARRICO ROBERT J

    IPC: C12Q1/68 ,  G01N20060101 ,  G01N33/53

    Abstract: A nucleic acid hybridization assay wherein the hybrid formed between the polynucleotide sequence to be detected and the probe is immobilized by binding of an immobilized or immobilized form of an antibody reagent selective for binding such hybrid. Preferred antibody reagents are selective for DNA?RNA or RNA?RNA hybrids or for intercalated duplexes. The immobilized hybrid is detected preferably by use of a labeled form of a second such antibody reagent or a labeled form of the probe. The method dispenses with the need to immobilize either the probe or sample nucleic acids and permits hybridization to proceed between the sequence to be detected and the probe in solution, resulting in rapid rates of hybridization.

    HYBRIDIZATION ASSAY EMPLOYING LABELED PROBE AND ANTI- HYBRID
    3.
    发明专利
    HYBRIDIZATION ASSAY EMPLOYING LABELED PROBE AND ANTI- HYBRID 未知

    公开(公告)号：CA1250230A

    公开(公告)日：1989-02-21

    申请号：CA469905

    申请日：1984-12-12

    Applicant: MILES LAB

    Inventor： CARRICO ROBERT J

    IPC: C12Q1/68 ,  G01N20060101 ,  G01N33/53

    Abstract: A nucleic acid hybridization assay involving a labeled probe and formation of a hybrid having epitopes for an antibody reagent. The label provides a detectable response which is measurably different when the labeled probe is comprised in a hybrid that is bound by the antibody reagent compared to when not comprised in such a hybrid. Particularly useful antibody reagents are antibodies such as anti-DNA'RNA, anti-RNA'RNA and antibodies to intercalated duplexes which do not bind substantially to single stranded nucleic acids. Modulation of the label response can be accomplished in a variety of ways such as by steric inactivation or hindrance or by labeling the antibody reagent with a second label which interacts with the first label. The assay is particularly advantageous because no separation of hybridized and unhybridized probe is required.

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