公开(公告)号：CA1231303A

公开(公告)日：1988-01-12

申请号：CA459077

申请日：1984-07-17

Applicant: MILES LAB

Inventor： CARRICO ROBERT J ,  DERIEMER LESLIE H ,  GROSCH JOSEPHINE C ,  WILSON GARY A

IPC: G01N33/50 ,  C12Q1/68 ,  G01N33/58

Abstract: A nucleic acid hybridization method for detecting bacteria in a test sample, such as a mammalian body fluid, employing a polynucleotide probe having a base sequence which is homologous with at least one base sequence in the nucleic acid content or substantially all of the respective bacteria suspected to be present in the sample. The homologous base sequence in the probe preferably comprises at least a 10 base portion of one of the strands of a gene which codes for the synthesis of a nucleic acid or protein comprised in the mechanism of protein synthesis, e.g., tRNAs, rRNAs, and elongation factors. Resulting hybridization between the probe and single stranded nucleic acids released from bacteria in the sample indicates bacterial presence and can be detected by employing labeled probes or otherwise detecting duplex formation. The method provides a unique, direct and rapid means for detecting the presence of bacteria without the need for conventional microbiological culturing. In particular, the method can be applied conveniently as a screening test for bacterial presence in fluids which are normally sterile, such as urine or blood.

公开(公告)号：DE3369702D1

公开(公告)日：1987-03-12

申请号：DE3369702

申请日：1983-10-19

Applicant: MILES LAB

Inventor： GROSCH JOSEPHINE ,  WILSON GARY A ,  WOLLWEBER KAREN ,  YEHLE CLIFFORD O

IPC: C12N1/02 ,  C12Q1/04

公开(公告)号：DK498983A

公开(公告)日：1984-05-02

申请号：DK498983

申请日：1983-10-31

Applicant: MILES LAB

Inventor： GROSCH JOSEPHINE ,  WILSON GARY A ,  WOLLWEBER KAREN ,  YEHLE CLIFFORD O

IPC: C12N1/02 ,  C12Q1/04 ,  C12Q ,  C12N

公开(公告)号：DK498883A

公开(公告)日：1984-05-02

申请号：DK498883

申请日：1983-10-31

Applicant: MILES LAB

Inventor： GROSCH JOSEPHINE ,  WILSON GARY A ,  WOLLWEBER KAREN ,  YEHLE CLIFFORD O

IPC: C12N15/09 ,  C12N1/20 ,  C12N9/28 ,  C12N9/34 ,  C12N9/54 ,  C12N9/92 ,  C12N15/00 ,  C12N15/64 ,  C12N15/75 ,  C12Q1/04 ,  C12Q1/40 ,  C12R1/07 ,  C12N

公开(公告)号：DK498883D0

公开(公告)日：1983-10-31

申请号：DK498883

申请日：1983-10-31

Applicant: MILES LAB

Inventor： GROSCH JOSEPHINE ,  WILSON GARY A ,  WOLLWEBER KAREN ,  YEHLE CLIFFORD O

IPC: C12N15/09 ,  C12N1/20 ,  C12N9/28 ,  C12N9/34 ,  C12N9/54 ,  C12N9/92 ,  C12N15/00 ,  C12N15/64 ,  C12N15/75 ,  C12Q1/04 ,  C12Q1/40 ,  C12R1/07 ,  C12N15

公开(公告)号：AU3138784A

公开(公告)日：1985-02-07

申请号：AU3138784

申请日：1984-08-01

Applicant: MILES LAB

Inventor： CARRICO ROBERT J ,  DERIEMER LESLIE H ,  GROSCH JOSEPHINE C ,  WILSON GARY A

IPC: G01N33/50 ,  C12Q1/68 ,  G01N33/58 ,  C07G17/00 ,  C12Q1/04 ,  C12Q1/12 ,  C12Q1/14 ,  C12R1/01 ,  C12R1/125 ,  C12R1/19 ,  C12R1/22 ,  C12R1/37 ,  C12R1/385

公开(公告)号：NO833808L

公开(公告)日：1984-05-02

申请号：NO833808

申请日：1983-10-19

Applicant: MILES LAB

Inventor： GROSCH JOSEPHINE ,  WILSON GARY A ,  WOLLWEBER KAREN ,  YEHLE CLIFFORD O

IPC: C12N15/09 ,  C12N1/20 ,  C12N9/28 ,  C12N9/34 ,  C12N9/54 ,  C12N9/92 ,  C12N15/00 ,  C12N15/64 ,  C12N15/75 ,  C12Q1/04 ,  C12Q1/40 ,  C12R1/07 ,  C12N

公开(公告)号：NO833809A

公开(公告)日：1984-05-02

申请号：NO833809

申请日：1983-10-19

Applicant: MILES LAB

Inventor： GROSCH JOSEPHINE ,  WILSON GARY A ,  WOLLWEBER KAREN ,  YEHLE CLIFFORD O

IPC: C12N1/02 ,  C12Q1/04 ,  C12Q

CPC classification number: C12Q1/04

公开(公告)号：AT25405T

公开(公告)日：1987-02-15

申请号：AT83110397

申请日：1983-10-19

Applicant: MILES LAB

Inventor： GROSCH JOSEPHINE ,  WILSON GARY A ,  WOLLWEBER KAREN ,  YEHLE CLIFFORD O

IPC: C12N1/02 ,  C12Q1/04

公开(公告)号：NO833809L

公开(公告)日：1984-05-02

申请号：NO833809

申请日：1983-10-19

Applicant: MILES LAB

Inventor： GROSCH JOSEPHINE ,  WILSON GARY A ,  WOLLWEBER KAREN ,  YEHLE CLIFFORD O

IPC: C12N1/02 ,  C12Q1/04 ,  C12Q