公开(公告)号：AU690273B2

公开(公告)日：1998-04-23

申请号：AU2491697

申请日：1997-06-17

Applicant: MITSUI TOATSU CHEMICALS

Inventor： IKEDA ICHIRO ,  KONO NAOKO ,  MAKINO TADASHI ,  HASHIMOTO YOSHIHIDE

IPC: C07K16/26 ,  G01N33/543 ,  G01N33/74 ,  G01N33/577

Abstract: A monoclonal antibody specifically reactive to a human growth hormone with a molecular weight of about 20,000, a cell line capable of producing the monoclonal antibody and an immunoassay of a human growth hormone having a molecular weight of about 20,000 using the monoclonal antibody. An animal cell capable of producing an antibody specific to a human growth hormone having a molecular weight of 20,000 (20k hGH) was prepared from an animal immunized with 20k hGH. The animal cell was fused with a myeloma cell to produce a monoclonal antibody which specifically reacted to 20k hGH, but substantially not to 22k hGH. The monoclonal antibody was useful for immunoassay of 20k hGH.

公开(公告)号：NZ328115A

公开(公告)日：1998-02-26

申请号：NZ32811597

申请日：1997-06-17

Applicant: MITSUI TOATSU CHEMICALS ,  MITSUI CHEMICALS INC

Inventor： IKEDA ICHIRO ,  KONO NAOKO ,  MAKINO TADASHI ,  HASHIMOTO YOSHIDE

IPC: C07K16/26 ,  G01N33/543 ,  G01N33/74 ,  C12N5/12 ,  C12N5/18 ,  C12N5/26 ,  C12N15/13 ,  C12N15/18 ,  C12N15/85 ,  C07K16/46 ,  C07K16/22 ,  C07K17/08 ,  C07K17/14 ,  C07K19/00 ,  G01N33/552 ,  G01N33/573 ,  C12P21/08 ,  C12Q1/68

Abstract: A monoclonal antibody specifically reactive to a human growth hormone with a molecular weight of about 20,000, a cell line capable of producing the monoclonal antibody and an immunoassay of a human growth hormone having a molecular weight of about 20,000 using the monoclonal antibody. An animal cell capable of producing an antibody specific to a human growth hormone having a molecular weight of 20,000 (20k hGH) was prepared from an animal immunized with 20k hGH. The animal cell was fused with a myeloma cell to produce a monoclonal antibody which specifically reacted to 20k hGH, but substantially not to 22k hGH. The monoclonal antibody was useful for immunoassay of 20k hGH.

公开(公告)号：EP0814091A2

公开(公告)日：1997-12-29

申请号：EP97304247

申请日：1997-06-17

Applicant: MITSUI TOATSU CHEMICALS

Inventor： IKEDA ICHIRO ,  KONO NAOKO ,  MAKINO TADASHI ,  HASHIMOTO YOSHIHIDE

IPC: C07K16/26 ,  G01N33/543 ,  G01N33/74 ,  C07K17/00 ,  C12N5/20 ,  G01N33/577

CPC classification number: C07K16/26 ,  G01N33/54306 ,  G01N33/74 ,  G01N2333/61 ,  Y10S530/809

Abstract: A monoclonal antibody specifically reactive to a human growth hormone with a molecular weight of about 20,000, a cell line capable of producing the monoclonal antibody and an immunoassay of a human growth hormone having a molecular weight of about 20,000 using the monoclonal antibody. An animal cell capable of producing an antibody specific to a human growth hormone having a molecular weight of 20,000 (20k hGH) was prepared from an animal immunized with 20k hGH. The animal cell was fused with a myeloma cell to produce a monoclonal antibody which specifically reacted to 20k hGH, but substantially not to 22k hGH. The monoclonal antibody was useful for immunoassay of 20k hGH.

Abstract translation: 一种单克隆抗体，与分子量为约20,000，一个细胞系能够产生单克隆抗体的人生长激素特异性反应和免疫测定使用单克隆抗体具有约20000的分子量的人生长激素。 动物细胞能够在特定的具有20,000（20k hGH的）的分子量，其从与20k hGH的免疫的动物制备的人生长激素抗体产生的。 动物细胞有什么骨髓瘤细胞融合以产生单克隆抗体具体在哪些的反应是20k hGH的，但基本上不22KhGH的。 单克隆抗体，其为20KhGH的免疫测定法是有用的。

公开(公告)号：JPH06237754A

公开(公告)日：1994-08-30

申请号：JP8276093

申请日：1993-03-17

Applicant: MITSUI TOATSU CHEMICALS

Inventor： AZUMA TATSUO ,  MURANAKA HIDEKAZU ,  AIKAWA TOSHIKAZU ,  KUSUHARA NOBUMI ,  SATOZAWA NOBORU ,  IKEDA ICHIRO

IPC: C12M1/00 ,  C12M3/06 ,  C12N5/04 ,  C12N5/07 ,  C12N5/071 ,  C12N5/06

Abstract: PURPOSE:To provide a cell separator enabling stable high-density culture for a long period of time without filter cloggings. CONSTITUTION:The objective cell separator, which is installed out of a culture tank and independently thereof, is equipped with (A) a tank to hold culture fluid and cells and (B) a rotatable filter with its cross-section of closed design, having meshes 1 to 5 times the size of a carrier in the case cells to be cultured or such carrier is used. As the meshes are larger than the size of cells, cloggings do not occur. Rotation of the filter makes apparent meshes smaller, resulting in adequate separatory efficiency for cells. With this separator, stable cell separation can be continued for one month or longer.

公开(公告)号：JPH06189781A

公开(公告)日：1994-07-12

申请号：JP34634292

申请日：1992-12-25

Applicant: MITSUI TOATSU CHEMICALS

Inventor： SHIMAZAKI YUKIO ,  FUKUDA TAMOTSU ,  KUSUHARA NOBUMI ,  AZUMA TATSUO ,  MURANAKA HIDEKAZU ,  TAKAGI SHIRO ,  SATOZAWA NOBORU ,  IKEDA ICHIRO ,  KAWASHIMA NOBUHIRO ,  AIKAWA TOSHIKAZU

IPC: C12N5/02 ,  C12N5/07 ,  C12N5/071 ,  C12P21/00 ,  C12P21/08 ,  C12R1/91

Abstract: PURPOSE:To recover a bioactive protein in a stable state, in production of the bioactive protein by perfusion culture of an animal cell. CONSTITUTION:pH of the recovered solution continuously obtained in perfusion culture of an animal cell is adjusted within a range of 6.0 to 7.0. Thereby, the bioactive protein can be prevented from being decomposed by a protease and the protein is stabilized.

公开(公告)号：JPH0723780A

公开(公告)日：1995-01-27

申请号：JP17142093

申请日：1993-07-12

Applicant: MITSUI TOATSU CHEMICALS

Inventor： SATOZAWA NOBORU ,  KUSUHARA NOBUMI ,  MURANAKA HIDEKAZU ,  IKEDA ICHIRO ,  HAYASHI TOMOKO

IPC: C12N5/10 ,  C12N15/02 ,  C12P21/08

Abstract: PURPOSE:To obtain a synthetic medium enabling the production of valuable substances by animal cells without using protein components such as transferrin, insulin and albumin by using glycylglycine as an essential component. CONSTITUTION:This synthetic medium for the high-density culture of animal cells to produce valuable substances contains glycylglycine and is absolutely free from proteins acting as proliferation factor (e.g. transferrin, insulin and albumin). The amount of glycylglycine added to the medium is about 1-100mM, preferably about 5-15mM.