公开(公告)号：EP1015869A4

公开(公告)日：2000-07-05

申请号：EP97941059

申请日：1997-09-12

Applicant: MOLECULAR DYNAMICS INC

Inventor： KAIN ROBERT C

IPC: G01N21/64 ,  G01N21/27 ,  G02B21/02 ,  G02B21/36 ,  G01N21/00 ,  G01N21/55 ,  G02B21/00

CPC classification number: G02B21/02

Abstract: The present invention features two flat-field, telecentric, infinite conjugate, achromatic objectives (32 and 34) each of which has an external pupil lying in a common plane located equidistant from the two objectives, defining a mechanically accessible central pupil (30) of an imaging system centered in the common plane (28). Each of the objectives is afocal in the common plane, with one of the lenses forming a focal plane proximate to a sample. The lenses are adapted to provide varying levels of magnification while keeping constant the number of resolvable points in the field of view. An array detector (14) is positioned proximate to a focal plane formed of the remaining objective lens (34). The double objective lens assembly is described as being included in transillumination and epi-illumination systems.

Abstract translation: 本发明的特征在于两个平场，远心，无限共轭消色差物镜（32和34），每个物镜具有位于与两个物镜等距离的共同平面中的外瞳，限定机械可接近的中心瞳孔（30）， 以公共平面（28）为中心的成像系统。 每个物镜在共同的平面上都是焦外的，其中一个镜片形成接近样品的焦平面。 镜头适用于提供不同级别的放大率，同时保持视场中可分辨点的数量不变。 阵列检测器（14）位于由剩余物镜（34）形成的焦平面附近。 双物镜组件被描述为包括在透照和落射照明系统中。

公开(公告)号：EP0792475A4

公开(公告)日：1999-09-22

申请号：EP96915535

申请日：1996-05-06

Applicant: MOLECULAR DYNAMICS INC

Inventor： MILLER MICHAEL F ,  MAJLOF LARS ,  KAIN ROBERT C

IPC: G01N21/62 ,  G01J3/44 ,  G01N21/17 ,  G01N21/27 ,  G01N21/64 ,  G01N21/76 ,  G01N21/84 ,  G02B26/10 ,  G03B42/00

CPC classification number: G01N21/6452 ,  G01J3/4406 ,  G01J2003/1213 ,  G01J2003/1221 ,  G02B26/10

Abstract: A compact, movable scan head (10) has multiple scanning modalities, capable of high speed and high resolution scanning of a variety of samples (20). Stimulation and detection of storage phosphor screens and fluorescent samples (20) are preferably achieved with first and second channels (81, 82) in an optical path of a first side (11) of the scan head (10). The first side (11) preferably has a laser diode light source (14). Reading of reflective and transmissive signals is also possible. A third channel (83) is available in the optical path of a second side (12) of the scan head (10). The third channel preferably provides LED (32) point scanning and reading of fluorescent, reflective and transmissive signals received from the sample (20). The various modalities of the scan head (10) have coincident optical paths.

公开(公告)号：EP1012578A4

公开(公告)日：2000-08-16

申请号：EP98904835

申请日：1998-01-29

Applicant: MOLECULAR DYNAMICS

Inventor： KAIN ROBERT C ,  MARASON ERIC G ,  JOHNSTON RICHARD F

IPC: G01N21/01 ,  G01N21/64

CPC classification number: G01N21/6456 ,  G01N21/6428 ,  G01N21/6452

Abstract: A sample substrate (51, Fig. 4A) for use in a fluorescence imaging system (Fig. 1) includes a rigid base with a specularly reflective surface, typically metal, on which is deposited a transparent coating layer (53). The coating layer has a thickness selected so that a particular fluorescence excitation wavelength, corresponding to a specified fluorescent constituent to be sought in sample material, has an optical path from the top of the coating layer to the reflecting surface in the base of substantially an odd multiple of one-quarter wavelength, so that the standing wave of the fluorescence excitation wavelength of light incident of the substrate has an antinode (61) located at or near where sample material would be disposed on top of the coating layer. This maximizes fluorescence excitation of the sample on the reflective substrate. The transparent coating layer may be a dielectric material (e.g. silica) or may be a multilayer structure with a top layer of biologically active material for binding a specified sample constituent.

Abstract translation: 用于荧光成像系统（图1）的样品基底（51，图4A）包括具有镜面反射表面（通常为金属）的刚性基底，在镜面反射表面上沉积透明涂层（53）。 涂层的厚度被选择为使得特定的荧光激发波长（对应于在样品材料中寻找的特定荧光成分）具有从涂层的顶部到基本上为奇数的基底中的反射表面的光路 使得基板入射的光的荧光激发波长的驻波具有位于或靠近样品材料将设置在涂层顶部上的波腹（61）。 这最大化了反射基底上样品的荧光激发。 透明涂层可以是电介质材料（例如二氧化硅），或者可以是具有生物活性材料顶层用于结合特定样品成分的多层结构。

公开(公告)号：EP0880690A4

公开(公告)日：2000-07-19

申请号：EP97904082

申请日：1997-01-31

Applicant: MOLECULAR DYNAMICS INC

Inventor： KAIN ROBERT C ,  ALEXAY CHRISTOPHER C

IPC: G01N21/64 ,  G02B21/00 ,  G02B21/16 ,  G02B21/36 ,  G01N21/55 ,  G02B21/02

CPC classification number: G01N21/6458 ,  G01N21/6428 ,  G01N2021/6421 ,  G01N2021/6463 ,  G02B21/002 ,  G02B21/0076

Abstract: Provided is a coaxial illumination and collection laser scanning system designed to provide substantially uniform detection sensitivity across the field of view of an objective lens (27; 127) by reducing lateral chromatic aberrations at the expense of amplifying axial chromatic aberrations. Axial chromatic aberrations in the system are removed in the path of a retro-beam (31). A laser (18; 117, 118) is in optical communication with the objective lens. The laser (18; 117, 118) produces a collimated beam of coherent light (19; 119) that is directed by a scanner (25) through the objective lens (27; 127) to illuminate a raster of spots on the sample's surface (29), thereby stimulating a series of small regions of the sample to emit light.

Abstract translation: 本发明涉及一种光学扫描方法，包括产生和组合第一和第二光束以形成入射光束（119）; 引导所述入射光束通过限定光轴和视野的物镜（127），并将入射光束（119）引导到样品（129）上，在其上产生照明光斑; 通过具有光学特性的光束扩展器（121）引导所述事件，以补偿由所述物镜（127）引入的轴向色差，从而确保来自所述第一光束和所述第二光束的基本上所有的光照射在所述样品的公共焦平面上 129）表面; 扫描样品（129）表面上的入射光束; 收集从所述光点发射的光并形成收集光的后光束，所述物镜（127）具有光学性质，以通过引入指定量的轴向色差来优化横向色彩，从而在所述视场上提供基本均匀的检测灵敏度 同时减小横向色差以提高所述视场中的检测灵敏度; 将入射光束（119）与逆光束分离; 通过具有光学特性的聚焦透镜引导分离的后光束光以补偿由物镜（127）引入的轴向色差，其中所述聚焦透镜通过空间滤光器上的孔将所述回射束聚焦; 以及检测通过所述空间滤波器的后光束光。

公开(公告)号：EP0746865A4

公开(公告)日：1999-09-01

申请号：EP95937560

申请日：1995-10-20

Applicant: MOLECULAR DYNAMICS INC

Inventor： KAIN ROBERT C

IPC: G01N21/64 ,  G02B21/00 ,  G02B21/16 ,  H01J3/14

CPC classification number: G02B21/002 ,  G02B21/16

Abstract: Fluorescence imaging system includes objective (21) which is achromatic and has an external entrance pupil (29), serves as a condenser for the system, and positioned above sample (23) in close proximity to one another. Laser (18) directs collimated light (19) to scan device (20) located at entrance pupil (29). Scan device (20) reflects, refracts, or diffracts light through objective (21) to illuminate a spot (22) on the sample's surface, and illuminates a line or an area on the sample surface by varying the angle of laser light into objective (21). Sample (23) emits fluorescent light (24) in response to the illumination. The fluorescent light (24) is collected by objective (21) and passes through the system along the path of the illumination light. Wavelength-discriminating dichroic filter (25), placed along the optical axis between laser (18) and objective (21), directs fluorescent light (24) onto photodetector (26) to produce a signal representing the sample surface emitting the fluorescent light. Display (37) displays the digitized data in a raster format.

Abstract translation: 荧光成像系统包括无色的物镜（21），其具有外部入射光瞳（29），用作系统的聚光器，并且位于样品（23）上方彼此靠近。 激光器（18）将准直光（19）引导到位于入瞳（29）处的扫描装置（20）。 扫描装置（20）通过物镜（21）反射，折射或衍射光线以照射样品表面上的斑点（22），并且通过将激光角度改变成物镜来照亮样品表面上的线或区域 21）。 样品（23）响应于照射而发射荧光（24）。 荧光灯（24）由物镜（21）收集并沿着照明光的路径通过系统。 沿着激光器（18）和物镜（21）之间的光轴放置的波长识别二向色滤光器（25）将荧光（24）引导到光探测器（26）上以产生表示发射荧光的样品表面的信号。 显示（37）以光栅格式显示数字化数据。

公开(公告)号：EP0770262A4

公开(公告)日：1998-09-09

申请号：EP96911747

申请日：1996-04-09

Applicant: MOLECULAR DYNAMICS INC

Inventor： KAIN ROBERT C ,  MILLER MICHAEL F ,  MAJLOF LARS

IPC: G01J1/04 ,  G01N21/01 ,  G01N21/47 ,  G01N21/64 ,  G02B26/10 ,  H01J3/14

CPC classification number: G01N21/6452

Abstract: An LED point imaging scanner for stimulating and reading fluorescent and reflective signal radiation from a target sample (10) is disclosed. LED light source (12) is mounted on scan head (20) and focused into pinhole aperture (15) of spatial filter (30), then collimated and focused to a spot (17) on sample (10). The spatial filter, or an optical fiber equivalent, effectively restricts the incoherent LED light and creates a point light source. Signal radiation from sample (10) is collected through scan head (20) and the returning light beam is collimated and focused onto detection means (40). A spatial filter (22) in the path of the returning beam may also be utilized. Detection means (40) is either a small detector directly mounted onto the scan head, or an end of an optical fiber which transmits the point image to a remotely-positioned stationary detector. The scan head is moved along one or two axes relative to the sample.

公开(公告)号：AU3963595A

公开(公告)日：1996-06-26

申请号：AU3963595

申请日：1995-10-20

Applicant: MOLECULAR DYNAMICS

Inventor： KAIN ROBERT C

IPC: G01N21/64 ,  G02B21/00 ,  G02B21/16 ,  H01J3/14

Abstract: A fluorescence imaging system that includes an objective that is both achromatic and has an external entrance pupil. The objective also serves as a condenser for the system which substantially reduces the system's cost and footprint. With the objective positioned above a sample so that they are in close proximity to one another, a laser directs a collimated beam of light to a scan device located at the objective entrance pupil. The scan device reflects, refracts, or diffracts the light through the lens to illuminate a spot on the sample's surface. The scan device illuminates a line or an area on the sample surface by varying the angle of laser light, in one or two dimensions, into the objective. The sample emits fluorescent light in response to the illumination. The fluorescence light is collected by the objective and passes through the system along the path of the illumination light. A wavelength-discriminating dichroic filter is placed along the optical axis between the laser and the objective to direct the fluorescent light onto a photo-detector to produce a signal representing the sample surface emitting the fluorescent light. A display device is provided that displays the digitized data in a raster format.