公开(公告)号：GB2368032B

公开(公告)日：2003-01-08

申请号：GB0201920

申请日：2000-08-02

Applicant: MOLECULAR DYNAMICS INC

Inventor： JOVANOVICH STEVAN B ,  ROACH DAVID J ,  HADD ANDREW G ,  HELLMAN BO E R

IPC: C12N15/09 ,  B01D57/02 ,  B01J4/00 ,  B01J19/00 ,  B01J19/26 ,  B01L3/00 ,  B01L7/00 ,  B01L99/00 ,  B03C5/00 ,  C12M1/00 ,  C12M1/34 ,  C12M1/38 ,  C12Q1/68 ,  C40B40/02 ,  C40B40/06 ,  C40B50/06 ,  C40B60/04 ,  C40B60/14 ,  G01N1/00 ,  G01N27/447 ,  G01N31/20 ,  G01N33/50 ,  G01N35/00 ,  G01N35/02 ,  G01N35/10 ,  G01N37/00

Abstract: Methods for preparing nanoscale reactions using nucleic acids are presented. Nucleic acids are captured saturably, yet reversibly, on the internal surface of the reaction chamber, typically a capillary. Excess nucleic acid is removed and the reaction is performed directly within the capillary. Alternatively, the saturably bound nucleic acid is eluted, dispensing a metered amount of nucleic acid for subsequent reaction in a separate chamber. Devices for effecting the methods of the invention and a system designed advantageously to utilize the methods for high throughput nucleic acid sequencing reactions are also provided.

公开(公告)号：CA2379969A1

公开(公告)日：2001-02-08

申请号：CA2379969

申请日：2000-08-02

Applicant: MOLECULAR DYNAMICS INC

Inventor： ROACH DAVID J ,  HADD ANDREW G ,  JOVANOVICH STEVAN B ,  HELLMAN BO E R

IPC: C12N15/09 ,  B01D57/02 ,  B01J4/00 ,  B01J19/00 ,  B01J19/26 ,  B01L3/00 ,  B01L7/00 ,  B01L99/00 ,  B03C5/00 ,  C12M1/00 ,  C12M1/34 ,  C12M1/38 ,  C12Q1/68 ,  C40B40/02 ,  C40B40/06 ,  C40B50/06 ,  C40B60/04 ,  C40B60/14 ,  G01N1/00 ,  G01N27/447 ,  G01N31/20 ,  G01N33/50 ,  G01N35/00 ,  G01N35/02 ,  G01N35/10 ,  G01N37/00

Abstract: An automated system utilizes an array of nanoscale capillary-dimension reaction chambers (12). The ends of the chambers are temporarily sealed with deformable membranes (264a, 264b) and the reactions effected by incubation o r temperature cycling. The reaction containers may be filled by capillary acti on and dispensed by air displacement, centrifugal force or other means. Reactio n mixtures may be assembled by using the reaction chambers (12) to meter reaction components that are combined on a substrate. Alternatively, a first reaction component may be immobilized on the interior surface of the reactio n container and a second mixture component pumped of drawn into the container to form a final reaction mixture.

公开(公告)号：GB2368032A

公开(公告)日：2002-04-24

申请号：GB0201920

申请日：2000-08-02

Applicant: MOLECULAR DYNAMICS INC

Inventor： JOVANOVICH STEVAN B ,  ROACH DAVID J ,  HADD ANDREW G ,  HELLMAN BO E R

IPC: C12N15/09 ,  B01D57/02 ,  B01J4/00 ,  B01J19/00 ,  B01J19/26 ,  B01L3/00 ,  B01L7/00 ,  B01L99/00 ,  B03C5/00 ,  C12M1/00 ,  C12M1/34 ,  C12M1/38 ,  C12Q1/68 ,  C40B40/02 ,  C40B40/06 ,  C40B50/06 ,  C40B60/04 ,  C40B60/14 ,  G01N1/00 ,  G01N27/447 ,  G01N31/20 ,  G01N33/50 ,  G01N35/00 ,  G01N35/02 ,  G01N35/10 ,  G01N37/00

Abstract: An automated system utilizes an array of nanoscale capillary-dimension reaction chambers (12). The ends of the chambers are temporarily sealed with deformable membranes (264a, 264b) and the reactions effected by incubation or temperature cycling. The reaction containers may be filled by capillary action and dispensed by air displacement, centrifugal force or other means. Reaction mixtures may be assembled by using the reaction chambers (12) to meter reaction components that are combined on a substrate. Alternatively, a first reaction component may be immobilized on the interior surface of the reaction container and a second mixture component pumped of drawn into the container to form a final reaction mixture.

公开(公告)号：AU6512700A

公开(公告)日：2001-02-19

申请号：AU6512700

申请日：2000-08-02

Applicant: MOLECULAR DYNAMICS INC

Inventor： JOVANOVICH STEVAN B ,  ROACH DAVID J ,  HADD ANDREW G ,  HELLMAN BO E R

IPC: C12N15/09 ,  B01D57/02 ,  B01J4/00 ,  B01J19/00 ,  B01J19/26 ,  B01L3/00 ,  B01L7/00 ,  B01L99/00 ,  B03C5/00 ,  C12M1/00 ,  C12M1/34 ,  C12M1/38 ,  C12Q1/68 ,  C40B40/02 ,  C40B40/06 ,  C40B50/06 ,  C40B60/04 ,  C40B60/14 ,  G01N1/00 ,  G01N27/447 ,  G01N31/20 ,  G01N33/50 ,  G01N35/00 ,  G01N35/02 ,  G01N35/10 ,  G01N37/00

Abstract: Methods for preparing nanoscale reactions using nucleic acids are presented. Nucleic acids are captured saturably, yet reversibly, on the internal surface of the reaction chamber, typically a capillary. Excess nucleic acid is removed and the reaction is performed directly within the capillary. Alternatively, the saturably bound nucleic acid is eluted, dispensing a metered amount of nucleic acid for subsequent reaction in a separate chamber. Devices for effecting the methods of the invention and a system designed advantageously to utilize the methods for high throughput nucleic acid sequencing reactions are also provided.