公开(公告)号：GB2368032B

公开(公告)日：2003-01-08

申请号：GB0201920

申请日：2000-08-02

Applicant: MOLECULAR DYNAMICS INC

Inventor： JOVANOVICH STEVAN B ,  ROACH DAVID J ,  HADD ANDREW G ,  HELLMAN BO E R

IPC: C12N15/09 ,  B01D57/02 ,  B01J4/00 ,  B01J19/00 ,  B01J19/26 ,  B01L3/00 ,  B01L7/00 ,  B01L99/00 ,  B03C5/00 ,  C12M1/00 ,  C12M1/34 ,  C12M1/38 ,  C12Q1/68 ,  C40B40/02 ,  C40B40/06 ,  C40B50/06 ,  C40B60/04 ,  C40B60/14 ,  G01N1/00 ,  G01N27/447 ,  G01N31/20 ,  G01N33/50 ,  G01N35/00 ,  G01N35/02 ,  G01N35/10 ,  G01N37/00

Abstract: Methods for preparing nanoscale reactions using nucleic acids are presented. Nucleic acids are captured saturably, yet reversibly, on the internal surface of the reaction chamber, typically a capillary. Excess nucleic acid is removed and the reaction is performed directly within the capillary. Alternatively, the saturably bound nucleic acid is eluted, dispensing a metered amount of nucleic acid for subsequent reaction in a separate chamber. Devices for effecting the methods of the invention and a system designed advantageously to utilize the methods for high throughput nucleic acid sequencing reactions are also provided.

公开(公告)号：CA2379969A1

公开(公告)日：2001-02-08

申请号：CA2379969

申请日：2000-08-02

Applicant: MOLECULAR DYNAMICS INC

Inventor： ROACH DAVID J ,  HADD ANDREW G ,  JOVANOVICH STEVAN B ,  HELLMAN BO E R

IPC: C12N15/09 ,  B01D57/02 ,  B01J4/00 ,  B01J19/00 ,  B01J19/26 ,  B01L3/00 ,  B01L7/00 ,  B01L99/00 ,  B03C5/00 ,  C12M1/00 ,  C12M1/34 ,  C12M1/38 ,  C12Q1/68 ,  C40B40/02 ,  C40B40/06 ,  C40B50/06 ,  C40B60/04 ,  C40B60/14 ,  G01N1/00 ,  G01N27/447 ,  G01N31/20 ,  G01N33/50 ,  G01N35/00 ,  G01N35/02 ,  G01N35/10 ,  G01N37/00

Abstract: An automated system utilizes an array of nanoscale capillary-dimension reaction chambers (12). The ends of the chambers are temporarily sealed with deformable membranes (264a, 264b) and the reactions effected by incubation o r temperature cycling. The reaction containers may be filled by capillary acti on and dispensed by air displacement, centrifugal force or other means. Reactio n mixtures may be assembled by using the reaction chambers (12) to meter reaction components that are combined on a substrate. Alternatively, a first reaction component may be immobilized on the interior surface of the reactio n container and a second mixture component pumped of drawn into the container to form a final reaction mixture.

公开(公告)号：GB2368032A

公开(公告)日：2002-04-24

申请号：GB0201920

申请日：2000-08-02

Applicant: MOLECULAR DYNAMICS INC

Inventor： JOVANOVICH STEVAN B ,  ROACH DAVID J ,  HADD ANDREW G ,  HELLMAN BO E R

IPC: C12N15/09 ,  B01D57/02 ,  B01J4/00 ,  B01J19/00 ,  B01J19/26 ,  B01L3/00 ,  B01L7/00 ,  B01L99/00 ,  B03C5/00 ,  C12M1/00 ,  C12M1/34 ,  C12M1/38 ,  C12Q1/68 ,  C40B40/02 ,  C40B40/06 ,  C40B50/06 ,  C40B60/04 ,  C40B60/14 ,  G01N1/00 ,  G01N27/447 ,  G01N31/20 ,  G01N33/50 ,  G01N35/00 ,  G01N35/02 ,  G01N35/10 ,  G01N37/00

Abstract: An automated system utilizes an array of nanoscale capillary-dimension reaction chambers (12). The ends of the chambers are temporarily sealed with deformable membranes (264a, 264b) and the reactions effected by incubation or temperature cycling. The reaction containers may be filled by capillary action and dispensed by air displacement, centrifugal force or other means. Reaction mixtures may be assembled by using the reaction chambers (12) to meter reaction components that are combined on a substrate. Alternatively, a first reaction component may be immobilized on the interior surface of the reaction container and a second mixture component pumped of drawn into the container to form a final reaction mixture.

公开(公告)号：AU6512700A

公开(公告)日：2001-02-19

申请号：AU6512700

申请日：2000-08-02

Applicant: MOLECULAR DYNAMICS INC

Inventor： JOVANOVICH STEVAN B ,  ROACH DAVID J ,  HADD ANDREW G ,  HELLMAN BO E R

IPC: C12N15/09 ,  B01D57/02 ,  B01J4/00 ,  B01J19/00 ,  B01J19/26 ,  B01L3/00 ,  B01L7/00 ,  B01L99/00 ,  B03C5/00 ,  C12M1/00 ,  C12M1/34 ,  C12M1/38 ,  C12Q1/68 ,  C40B40/02 ,  C40B40/06 ,  C40B50/06 ,  C40B60/04 ,  C40B60/14 ,  G01N1/00 ,  G01N27/447 ,  G01N31/20 ,  G01N33/50 ,  G01N35/00 ,  G01N35/02 ,  G01N35/10 ,  G01N37/00

Abstract: Methods for preparing nanoscale reactions using nucleic acids are presented. Nucleic acids are captured saturably, yet reversibly, on the internal surface of the reaction chamber, typically a capillary. Excess nucleic acid is removed and the reaction is performed directly within the capillary. Alternatively, the saturably bound nucleic acid is eluted, dispensing a metered amount of nucleic acid for subsequent reaction in a separate chamber. Devices for effecting the methods of the invention and a system designed advantageously to utilize the methods for high throughput nucleic acid sequencing reactions are also provided.

公开(公告)号：EP0991472A4

公开(公告)日：2000-08-16

申请号：EP97928828

申请日：1997-06-03

Applicant: MOLECULAR DYNAMICS INC

Inventor： ROACH DAVID J ,  JOHNSTON RICHARD F

IPC: G01N35/10 ,  B01L3/02 ,  C40B60/14

CPC classification number: B01L3/0262 ,  B01J2219/00387 ,  B01J2219/00527 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00639 ,  B01J2219/00659 ,  B01J2219/0072 ,  B01L3/0248 ,  B01L3/0255 ,  B01L2300/0838 ,  C40B60/14 ,  G01N2035/1037 ,  Y10T436/25 ,  Y10T436/2575

Abstract: A microspot deposition system (12) featuring a hollow cylindrical wall (26) extending from a closed end (28), terminating in an open end (30) and including a longitudinal gap (34) extending from the open end (30) toward the closed end (28) to allow the rapid exhaustion of the atmosphere and efficient cleaning within the cylindrical wall (26). The cylindrical wall (26) defines a lumen (32) with both the lumen (32) and the gap (34) adapted to facilitate capillary action of liquid in fluid communication therewith to form a meniscus (46) proximate to the open end (30). To facilitate deposition of liquid contained within the lumen (32), the gap (34) may be tapered so that it is narrowest proximate to the open end (30). The narrowed portion of the gap (34) results in a meniscus (46) having a reduced area to ensure preferential fluid flow toward the open end (30), which facilitates deposition via capillary action between the liquid in the lumen (32) and a working surface (52).

Abstract translation: 1。一种微点沉积系统（12），其特征在于从封闭端（28）延伸的中空圆柱形壁（26），终止于开口端（30）并包括从开口端（30）朝向 封闭端（28）以允许大气的快速消耗和圆柱形壁（26）内的有效清洁。 圆柱形壁（26）限定内腔（32），内腔（32）和间隙（34）适于促进与其流体连通的液体的毛细作用，以形成接近开口端（30）的弯月面（46） ）。 为了促进容纳在内腔（32）内的液体的沉积，间隙（34）可以是锥形的，使得它在开口端（30）附近最窄。 间隙（34）的变窄部分导致弯液面（46）具有减小的面积以确保朝向开口端（30）的优先流体流动，这便于通过腔（32）中的液体与 工作表面（52）。