公开(公告)号：AT236719T

公开(公告)日：2003-04-15

申请号：AT99951826

申请日：1999-10-07

Applicant: MOTOROLA INC

Inventor： BURDON JEREMY W ,  HUANG RONG-FONG ,  WILCOX DAVID ,  NACLERIO NICHOLAS J ,  BRISCOE CYNTHIA ANN GORSUCH ,  GRODZINSKI PIOTR ,  YU HUINAN ,  MARRERO ROBERT ,  GALLAGHER SEAN ROSS ,  CHAN YUK-TONG ,  FOLEY BARBARA BARENBURG ,  DAI XUNHU

IPC: G01N35/08 ,  B01J19/00 ,  B01J19/24 ,  B01J35/02 ,  B01L3/00 ,  B01L7/00 ,  B28B11/00 ,  B32B18/00 ,  B81B1/00 ,  C12M1/00 ,  C40B60/14 ,  F04B19/00 ,  F28D9/00 ,  F28F13/00 ,  G01N30/02 ,  G01N30/52 ,  G01N30/60 ,  G01N35/00 ,  G01N37/00 ,  H05K1/02 ,  H05K1/03 ,  H05K3/46

Abstract: A multilayered microfluidic DNA analysis system includes a cell lysis chamber, a DNA separation chamber, a DNA amplification chamber, and a DNA detection system. The multilayered microfluidic DNA analysis system is provided as a substantially monolithic structure formed from a plurality of green-sheet layers sintered together. The substantially monolithic structure has defined therein a means for heating the DNA amplification chamber and a means for cooling the DNA amplification chamber. The means for heating and means for cooling operate to cycle the temperature of the DNA amplification chamber as required for performing a DNA amplification process, such as PCR.

公开(公告)号：ES2197681T3

公开(公告)日：2004-01-01

申请号：ES99951826

申请日：1999-10-07

Applicant: MOTOROLA INC

Inventor： BURDON JEREMY W ,  HUANG RONG-FONG ,  WILCOX DAVID ,  NACLERIO NICHOLAS J ,  BRISCOE CYNTHIA ANN GORSUCH ,  GRODZINSKI PIOTR

IPC: G01N35/08 ,  B01J19/00 ,  B01J19/24 ,  B01J35/02 ,  B01L3/00 ,  B01L7/00 ,  B28B11/00 ,  B32B18/00 ,  B81B1/00 ,  C12M1/00 ,  C40B60/14 ,  F04B19/00 ,  F28D9/00 ,  F28F13/00 ,  G01N30/02 ,  G01N30/52 ,  G01N30/60 ,  G01N35/00 ,  G01N37/00 ,  H05K1/02 ,  H05K1/03 ,  H05K3/46

Abstract: A multilayered microfluidic DNA analysis system includes a cell lysis chamber, a DNA separation chamber, a DNA amplification chamber, and a DNA detection system. The multilayered microfluidic DNA analysis system is provided as a substantially monolithic structure formed from a plurality of green-sheet layers sintered together. The substantially monolithic structure has defined therein a means for heating the DNA amplification chamber and a means for cooling the DNA amplification chamber. The means for heating and means for cooling operate to cycle the temperature of the DNA amplification chamber as required for performing a DNA amplification process, such as PCR.

公开(公告)号：AU6418499A

公开(公告)日：2000-05-01

申请号：AU6418499

申请日：1999-10-07

Applicant: MOTOROLA INC

Inventor： BURDON JEREMY W ,  HUANG RONG-FONG ,  WILCOX DAVID ,  NACLERIO NICHOLAS J ,  BRISCOE CYNTHIA ANN GORSUCH ,  GRODZINSKI PIOTR ,  YU HUINAN ,  MARRERO ROBERT ,  GALLAGHER SEAN ROSS ,  CHAN YUK-TONG ,  FOLEY BARBARA MCNEIL ,  DAI XUNHU

IPC: G01N35/08 ,  B01J19/00 ,  B01J19/24 ,  B01J35/02 ,  B01L3/00 ,  B01L7/00 ,  B28B11/00 ,  B32B18/00 ,  B81B1/00 ,  C12M1/00 ,  C40B60/14 ,  F04B19/00 ,  F28D9/00 ,  F28F13/00 ,  G01N30/02 ,  G01N30/52 ,  G01N30/60 ,  G01N35/00 ,  G01N37/00 ,  H05K1/02 ,  H05K1/03 ,  H05K3/46

Abstract: A multilayered microfluidic DNA analysis system includes a cell lysis chamber, a DNA separation chamber, a DNA amplification chamber, and a DNA detection system. The multilayered microfluidic DNA analysis system is provided as a substantially monolithic structure formed from a plurality of green-sheet layers sintered together. The substantially monolithic structure has defined therein a means for heating the DNA amplification chamber and a means for cooling the DNA amplification chamber. The means for heating and means for cooling operate to cycle the temperature of the DNA amplification chamber as required for performing a DNA amplification process, such as PCR.

公开(公告)号：CA2346059A1

公开(公告)日：2000-04-20

申请号：CA2346059

申请日：1999-10-07

Applicant: MOTOROLA INC

Inventor： GRODZINSKI PIOTR ,  GALLAGHER SEAN ROSS ,  BURDON JEREMY W ,  FOLEY BARBARA MCNEIL ,  HUANG RONG-FONG ,  DAI XUNHU ,  BRISCOE CYNTHIA ANN GORSUCH ,  NACLERIO NICHOLAS J ,  YU HUINAN ,  MARRERO ROBERT ,  WILCOX DAVID ,  CHAN YUK-TONG

IPC: G01N35/08 ,  B01J19/00 ,  B01J19/24 ,  B01J35/02 ,  B01L3/00 ,  B01L7/00 ,  B28B11/00 ,  B32B18/00 ,  B81B1/00 ,  C12M1/00 ,  C40B60/14 ,  F04B19/00 ,  F28D9/00 ,  F28F13/00 ,  G01N30/02 ,  G01N30/52 ,  G01N30/60 ,  G01N35/00 ,  G01N37/00 ,  H05K1/02 ,  H05K1/03 ,  H05K3/46

Abstract: A multilayered microfluidic device having a substantially monolithic structu re is formed by sintering together a plurality of green-sheet layers. The substantially monolithic structure has an inlet port for receiving fluid, an outlet port for releasing fluid, and an interconnection between the inlet po rt and the outlet port. The substantially monolithic structure may also include a variety of components to enable useful interaction with the fluid, such as electrically conductive pathways, heaters, fluid sensors, fluid motion transducers, and optically transmissive portions. The components are preferably fabricated using thick-film or green-sheet technology and are preferably co-fired with and sintered to the green-sheet layers to become integral with the substantially monolithic structure. By using an adhesive t o bind the green-sheet layers together, the multilayered microfluidic device m ay be fabricated without the application of high pressures. Selection of an adhesive with a polymer that decomposes at a higher temperature than the binder present in the green-sheet layers promotes stability of the interface s during the firing process and promotes void-free sintering within the interfacial regions.

公开(公告)号：WO0021659A9

公开(公告)日：2000-09-08

申请号：PCT/US9923324

申请日：1999-10-07

Applicant: MOTOROLA INC

Inventor： BURDON JEREMY W ,  HUANG RONG-FONG ,  WILCOX DAVID ,  NACLERIO NICHOLAS J ,  BRISCOE CYNTHIA ANN GORSUCH ,  GRODZINSKI PIOTR ,  YU HUINAN ,  MARRERO ROBERT ,  GALLAGHER SEAN ROSS ,  CHAN YUK-TONG ,  FOLEY BARBARA MCNEIL ,  DAI XUNHU

IPC: G01N35/08 ,  B01J19/00 ,  B01J19/24 ,  B01J35/02 ,  B01L3/00 ,  B01L7/00 ,  B28B11/00 ,  B32B18/00 ,  B81B1/00 ,  C12M1/00 ,  C40B60/14 ,  F04B19/00 ,  F28D9/00 ,  F28F13/00 ,  G01N30/02 ,  G01N30/52 ,  G01N30/60 ,  G01N35/00 ,  G01N37/00 ,  H05K1/02 ,  H05K1/03 ,  H05K3/46

CPC classification number: G01N30/6069 ,  B01J19/0093 ,  B01J2219/00317 ,  B01J2219/00351 ,  B01J2219/00495 ,  B01J2219/00783 ,  B01J2219/00835 ,  B01J2219/00873 ,  B01J2219/00966 ,  B01L3/502707 ,  B01L3/50273 ,  B01L3/502738 ,  B01L7/00 ,  B01L7/52 ,  B01L2200/0689 ,  B01L2200/12 ,  B01L2300/0627 ,  B01L2300/0645 ,  B01L2300/0874 ,  B01L2300/0883 ,  B01L2300/0887 ,  B01L2300/12 ,  B01L2300/18 ,  B01L2300/1822 ,  B01L2400/0415 ,  B01L2400/0439 ,  B32B18/00 ,  B32B2038/042 ,  B32B2311/06 ,  B32B2311/08 ,  B32B2315/02 ,  B81B2201/058 ,  B81C1/00119 ,  B81C2201/019 ,  C04B2235/6562 ,  C04B2237/341 ,  C04B2237/343 ,  C04B2237/348 ,  C04B2237/50 ,  C04B2237/62 ,  C04B2237/68 ,  C04B2237/704 ,  C40B60/14 ,  F04B19/006 ,  F28D9/00 ,  F28D2021/0029 ,  F28F13/00 ,  F28F2260/02 ,  G01N30/60 ,  G01N30/6004 ,  G01N30/6095 ,  G01N2030/025 ,  G01N2030/528 ,  G01N2035/00158 ,  G01N2035/00237 ,  H05K1/0272 ,  H05K1/0306 ,  H05K3/4611 ,  H05K3/4629

Abstract: A multilayered microfluidic device having a substantially monolithic structure is formed by sintering together a plurality of green-sheet layers. The substantially monolithic structure has an inlet port for receiving fluid, an outlet port for releasing fluid, and an interconnection between the inlet port and the outlet port. The substantially monolithic structure may also include a variety of components to enable useful interaction with the fluid, such as electrically conductive pathways, heaters, fluid sensors, fluid motion transducers, and optically transmissive portions. The components are preferably fabricated using thick-film or green-sheet technology and are preferably co-fired with and sintered to the green-sheet layers to become integral with the substantially monolithic structure. By using an adhesive to bind the green-sheet layers together, the multilayered microfluidic device may be fabricated without the application of high pressures. Selection of an adhesive with a polymer that decomposes at a higher temperature than the binder present in the green-sheet layers promotes stability of the interfaces during the firing process and promotes void-free sintering within the interfacial regions.

Abstract translation: 具有基本上整体结构的多层微流体装置通过将多个绿色层层烧结在一起形成。 基本上单片结构具有用于接收流体的入口端口，用于释放流体的出口端口以及入口端口和出口端口之间的互连。 基本上单片结构还可以包括各种部件，以使得能够与诸如导电通路，加热器，流体传感器，流体运动换能器和光学透射部分之类的流体有用的相互作用。 这些组分优选使用厚膜或生片技术制造，并且优选与生片层共烧并烧结成与基本上一体的结构一体。 通过使用粘合剂将生片层结合在一起，可以在不施加高压的情况下制造多层微流体装置。 使用聚合物粘合剂的选择在温度高于生物层中存在的粘合剂的温度下分解促进了烧结过程中界面的稳定性，并促进界面区域内的无空隙烧结。