公开(公告)号：US11357806B2

公开(公告)日：2022-06-14

申请号：US15131218

申请日：2016-04-18

Applicant: Macau University of Science and Technology

Inventor： Zhi-Hong Jiang ,  Jing-Rong Wang ,  Jia-Ning Mi ,  Junping Kou ,  Yuwei Han ,  Yingqiong Xu

IPC: A61K36/068 ,  A61K31/133 ,  A61K31/164 ,  A61K31/688 ,  G01N30/34 ,  G01N30/72 ,  B01D15/42 ,  C12Q1/04 ,  G01N33/92 ,  G01N30/00 ,  G01N30/02

Abstract: A method of isolating at least one sphingolipid portion selected from a sphingoid base portion, a ceramide portion, a glycosphingolipid portion or a phosphosphingolipid portion from Cordyceps, in particular from wild-type Cordyceps, allows for obtaining sphingolipid portions having an increased amount of one of sphingoid bases, ceramides, glycosphingolipids or phosphosphingolipids. The sphingolipid portions isolated contained significant amounts of sphingolipids not reported so far, and possess exceptional immunosuppressive activities. A method of treating a subject suffering from an inflammatory disease like an autoimmune disease or an allergic disease includes administering sphingolipids isolated from Cordyceps, in particular from wild-type Cordyceps. A method of treating a subject suffering from an inflammatory disease includes administering certain sphingolipids to the subject. Still further in accordance with the present invention is a composition, in particular a pharmaceutical composition comprising at least one sphingolipid portion.

公开(公告)号：US10039797B2

公开(公告)日：2018-08-07

申请号：US15131243

申请日：2016-04-18

Applicant: Macau University of Science and Technology

Inventor： Jing-Rong Wang ,  Jia-Ning Mi ,  Zhi-Hong Jiang

IPC: A61K36/068 ,  A61K31/133 ,  A61K31/164 ,  A61K31/688 ,  B01D15/42 ,  C12Q1/04 ,  G01N30/02 ,  G01N30/72 ,  G01N33/92

Abstract: A method of identifying and preferably quantifying at least one sphingolipid, in particular the sphingolipid portion in wild-type Cordyceps or Cordyceps derivates includes preparing a test sample solution from a Cordyceps sample, subjecting the test sample solution to liquid chromatrography with a mobile phase including at least a first and second eluting solvent; and performing a mass spectrometry after the step of subjecting. The quality and safety of Cordyceps products can be determined and wild-type Cordyceps and Cordyceps derivates may be differentiated via the ratio and/or presence of certain sphingolipids especially suitable as markers.

公开(公告)号：US20170131300A1

公开(公告)日：2017-05-11

申请号：US15131243

申请日：2016-04-18

Applicant: Macau University of Science and Technology

Inventor： Jing-Rong Wang ,  Jia-Ning Mi ,  Zhi-Hong Jiang

IPC: G01N33/92 ,  G01N30/72 ,  B01D15/42 ,  C12Q1/04

CPC classification number: A61K36/068 ,  A61K31/133 ,  A61K31/164 ,  A61K31/688 ,  A61K2236/333 ,  A61K2236/39 ,  A61K2236/50 ,  A61K2236/53 ,  B01D15/426 ,  C12Q1/04 ,  G01N30/72 ,  G01N33/92 ,  G01N2030/027 ,  G01N2333/37 ,  G01N2405/08

Abstract: The present invention relates to a method of identifying and preferably quantifying at least one sphingolipid, in particular the sphingolipid portion in wild-type Cordyceps or Cordyceps derivates. A further aspect relates to a method of identifying wild-type Cordyceps in a Cordyceps sample as well as for identifying Cordyceps derivates in a Cordyceps sample. The methods of the present invention, thus, allow for determining the quality and safety of Cordyceps products and for advantageously differentiating between wild-type Cordyceps and Cordyceps derivates via the ratio and/or presence of certain sphingolipids especially suitable as markers.

公开(公告)号：US20170128507A1

公开(公告)日：2017-05-11

申请号：US15131218

申请日：2016-04-18

Applicant: Macau University of Science and Technology

Inventor： Zhi-Hong Jiang ,  Jing-Rong Wang ,  Jia-Ning Mi ,  Junping Kou ,  Yuwei Han ,  Yingqiong Xu

IPC: A61K36/068 ,  A61K31/164 ,  A61K31/688 ,  A61K31/133

CPC classification number: A61K36/068 ,  A61K31/133 ,  A61K31/164 ,  A61K31/688 ,  A61K2236/333 ,  A61K2236/39 ,  A61K2236/50 ,  A61K2236/53 ,  B01D15/426 ,  C12Q1/04 ,  G01N30/34 ,  G01N30/72 ,  G01N30/7233 ,  G01N33/92 ,  G01N2030/009 ,  G01N2030/027 ,  G01N2333/37 ,  G01N2405/08

Abstract: A method of isolating at least one sphingolipid portion selected from a sphingoid base portion, a ceramide portion, a glycosphingolipid portion or a phosphosphingolipid portion from Cordyceps, in particular from wild-type Cordyceps, allows for obtaining sphingolipid portions having an increased amount of one of sphingoid bases, ceramides, glycosphingolipids or phosphosphingolipids. The sphingolipid portions isolated contained significant amounts of sphingolipids not reported so far, and possess exceptional immunosuppressive activities. A method of treating a subject suffering from an inflammatory disease like an autoimmune disease or an allergic disease includes administering sphingolipids isolated from Cordyceps, in particular from wild-type Cordyceps. A method of treating a subject suffering from an inflammatory disease includes administering certain sphingolipids to the subject. Still further in accordance with the present invention is a composition, in particular a pharmaceutical composition comprising at least one sphingolipid portion.