公开(公告)号：EP1645563A4

公开(公告)日：2006-09-06

申请号：EP04747133

申请日：2004-07-07

Applicant: NAT INST OF ADVANCED IND SCIEN

Inventor： MIZUKAMI FUJIO ,  KIYOZUMI Y ,  IKEDA TAKUJI ,  KAWAI AKIKO ,  NAGASE TAKAKO ,  SAKAGUCHI KENGO ,  CHIKU HIROYUKI

IPC: C07K1/113 ,  C07K14/245

CPC classification number: C07K1/1133 ,  C07K1/1136 ,  C07K14/245

Abstract: It is intended to provide a method of refolding an inactive protein which is produced by Escherichia coli or the like and the higher-order structure of which is still unformed or a protein which has been activated due to a change in its three-dimensional structure for some reason, etc. Namely, a method comprising treating an inactive protein which is produced by Escherichia coli or the like and the higher-order structure of which is still unformed or a protein which has been activated due to a change in its three-dimensional structure for some reason with zeolite beta to thereby impart the inherent function or activity to the protein; a refolding kit; a refolding agent and a molded article; and a method of producing an active protein with the use of the same. Thus, it is possible to provide a novel method of activating protein function that is excellent in versatility and universality, can be conveniently and easily carried out, is less expensive and allows repeated use of a function activator, compared with the existing methods.

公开(公告)号：EP1688393A4

公开(公告)日：2009-05-06

申请号：EP04818561

申请日：2004-11-17

Applicant: NAT INST OF ADVANCED IND SCIEN

Inventor： IKEDA TAKUJI ,  KOMURA KENICHI ,  MIZUKAMI FUJIO ,  NIWA SYUICHI ,  YOKOYAMA TOSHIROU ,  HANAOKA TAKAAKI ,  SATO KOICHI ,  KIYOZUMI YOSHIMICHI ,  HASEGAWA YASUHISA ,  MANICKAM SASIDHARAN

IPC: C01B39/46 ,  B01D67/00 ,  B01D71/02 ,  B01J29/04 ,  B01J29/70 ,  C01B37/02 ,  C07D201/04 ,  C07D223/10

CPC classification number: B01D71/028 ,  B01D67/0051 ,  B01D67/0083 ,  B01D2323/08 ,  B01D2325/02 ,  B01D2325/22 ,  B01J29/04 ,  B01J29/70 ,  C01B37/02 ,  C07D223/10

Abstract: Disclosed are a high silica zeolite having a novel crystal structure, a zeolite membrane and methods for producing them. The zeolite is represented by the chemical composition:[(Si36-xTy·O72)·Mz] (wherein M represents a cation of an alkali metal such as Li, Na, K and Rb; T represents Al, Ga, Fe and Ce as skeleton substituting elements; and x satisfies 0 ≤ x ≤ 3.0, y satisfies 0 ≤ y ≤ 1.0, and z satisfies 0 ≤ z ≤ 3.0), and has a micropore composed of a covalent bond of Si-O. The zeolite has a specific diffraction peak at 2θ in the powder x-ray diffraction pattern.

公开(公告)号：AT540900T

公开(公告)日：2012-01-15

申请号：AT04818561

申请日：2004-11-17

Applicant: NAT INST OF ADVANCED IND SCIEN

Inventor： IKEDA TAKUJI ,  KOMURA KENICHI ,  MIZUKAMI FUJIO ,  NIWA SYUICHI ,  YOKOYAMA TOSHIROU ,  HANAOKA TAKAAKI ,  SATO KOICHI ,  KIYOZUMI YOSHIMICHI ,  HASEGAWA YASUHISA ,  MANICKAM SASIDHARAN

IPC: C01B39/46 ,  B01D67/00 ,  B01D71/02 ,  B01J29/04 ,  B01J29/70 ,  C01B37/02 ,  C07D201/04 ,  C07D223/10

Abstract: A high-silica content zeolite having a novel crystal structure, a zeolite membrane and manufacturing methods for these are provided, and the present invention relates to a zeolite having the chemical composition represented by [(Si 36-x T y ·O 72 )·M z ] (wherein M is a cation of an alkali metal such as Li, Na, K or Rb, T represents Al, Ga, Fe and Ce as skeleton substituting elements, x satisfies 0 ‰¤ x ‰¤ 3.0, y satisfies 0 ‰¤ y ‰¤ 1.0 and z satisfies 0 ‰¤ z ‰¤ 3.0), and having a micropore formed of covalent bonds between Si and O atoms, with a specific diffraction peak at 2¸ in powder x-ray diffraction, together with a zeolite membrane and methods for manufacturing these.

公开(公告)号：CA2531969C

公开(公告)日：2011-04-26

申请号：CA2531969

申请日：2004-07-07

Applicant: NAT INST OF ADVANCED IND SCIEN

Inventor： MIZUKAMI FUJIO ,  KIYOZUMI YOSHIMICHI ,  IKEDA TAKUJI ,  KAWAI AKIKO ,  NAGASE TAKAKO ,  SAKAGUCHI KENGO ,  CHIKU HIROYUKI

IPC: C07K1/113 ,  C07K14/245

Abstract: The present invention provides a method for refolding protein produced, for example, by Escherichia coli, that is inactive due to an as yet unformed higher order structure, or protein deactivated due to a change in conformation for some reason. The invention comprises a method, refolding kit, refolding agent, and molding that activate a native function or activity inherent to a protein through treatment with zeolite beta of protein produced, for example, by Escherichia coli, that is inactive due to an as yet unformed higher order structure, or protein deactivated due to a change in conformation for some reason. The invention also comprises a method for producing an active protein that utilizes the same. As compared with conventional methods, the present invention can provide a novel method for activating protein function that is highly versatile and generalizable, that employs a simple and easy protocol, and that is inexpensive and enables repeated use of the function activator.

公开(公告)号：CA2531969A1

公开(公告)日：2005-01-20

申请号：CA2531969

申请日：2004-07-07

Applicant: NAT INST OF ADVANCED IND SCIEN

Inventor： SAKAGUCHI KENGO ,  CHIKU HIROYUKI ,  IKEDA TAKUJI ,  KAWAI AKIKO ,  NAGASE TAKAKO ,  KIYOZUMI YOSHIMICHI ,  MIZUKAMI FUJIO

IPC: C07K1/113 ,  C07K14/245

Abstract: It is intended to provide a method of refolding an inactive protein which is produced by Escherichia coli or the like and the higher-order structure of which is still unformed or a protein which has been activated due to a chang e in its three-dimensional structure for some reason, etc. Namely, a method comprising treating an inactive protein which is produced by Escherichia col i or the like and the higher-order structure of which is still unformed or a protein which has been activated due to a change in its three-dimensional structure for some reason with zeolite beta to thereby impart the inherent function or activity to the protein; a refolding kit; a refolding agent and a molded article; and a method of producing an active protein with the use of the same. Thus, it is possible to provide a novel method of activating prote in function that is excellent in versatility and universality, can be conveniently and easily carried out, is less expensive and allows repeated u se of a function activator, compared with the existing methods.