Abstract:
It is an object of the present invention to provide: a protein refolding column filler, which is effective for the refolding, namely, the activation of the function, of an inactive protein with an as yet unformed higher order structure produced in Escherichia coli or the like, or a protein whose conformation has been changed due to a certain cause and which has become inactivated; and a column filled with the aforementioned column filler. The present invention provides a protein refolding column filler, which comprises zeolite with BEA structure (Zeolite Beta) that is granulated into a particle state.
Abstract:
It is intended to provide a method of refolding an inactive protein which is produced by Escherichia coli or the like and the higher-order structure of which is still unformed or a protein which has been activated due to a change in its three-dimensional structure for some reason, etc. Namely, a method comprising treating an inactive protein which is produced by Escherichia coli or the like and the higher-order structure of which is still unformed or a protein which has been activated due to a change in its three-dimensional structure for some reason with zeolite beta to thereby impart the inherent function or activity to the protein; a refolding kit; a refolding agent and a molded article; and a method of producing an active protein with the use of the same. Thus, it is possible to provide a novel method of activating protein function that is excellent in versatility and universality, can be conveniently and easily carried out, is less expensive and allows repeated use of a function activator, compared with the existing methods.
Abstract:
An enzyme electrode having excellent sensitivity, excellent stability, and a longer operating life, and an enzyme sensor using the enzyme electrode are provided. The enzyme electrode includes an electrode 2, a mesoporous silica material 3 formed on the electrode 2, and enzyme 4 immobilized in a small cavity of the mesoporous silica material 3. The size of the small cavity of the mesoporous silica material 3 is set to be 0.5 to 2.0 times the size of the enzyme 4.
Abstract:
The present invention provides a clay film and a member thereof, having low moisture permeability and gas permeability, having mechanical strength enabling use as a self-supporting film, having high flexibility and high heat resistance, being an electrical insulator and having low thermal conductivity; a clay film comprising clay alone, clay and a small amount of a reinforcing material, or clay, a small amount of an additive and a small amount of a reinforcing material and subjected to surface treatment; a clay film comprising clay and a small amount of a reinforcing material, or clay, a small amount of an additive and a small amount of a fibrous reinforcing material, and having gas impermeability; a multilayer film comprising a fabric and clay and having a composite multilayer structure comprising a clay layer and a fabric; and, an adhesive clay film having clay for a main component thereof and a single layer or multilayer structure in which an adhesive layer is present on the clay film.
Abstract:
Disclosed are a high silica zeolite having a novel crystal structure, a zeolite membrane and methods for producing them. The zeolite is represented by the chemical composition:[(Si36-xTy·O72)·Mz] (wherein M represents a cation of an alkali metal such as Li, Na, K and Rb; T represents Al, Ga, Fe and Ce as skeleton substituting elements; and x satisfies 0 ≤ x ≤ 3.0, y satisfies 0 ≤ y ≤ 1.0, and z satisfies 0 ≤ z ≤ 3.0), and has a micropore composed of a covalent bond of Si-O. The zeolite has a specific diffraction peak at 2θ in the powder x-ray diffraction pattern.
Abstract:
A method for carrying out a reaction of one substance capable of being activated by a catalyst with another substance capable of reacting with said one substance activated, characterized in that the substance capable of bein g activated is activated by passing the substance through a diaphram type catalyst and the reaction is thus performed in one reaction step; a method f or producing an aromatic alcohol utilizing the above method; and a reaction apparatus suitable for these reactions. In the method, one substance is activated by passing through a diaphram type catalyst and an objective reaction is carried out by using the activated substance, and the reaction c an be performed in one reaction step and with safety. Moreover, the contact of the above activated substance with a compound to be reacted therewith can be freely controlled, and therefore, over-reaction can be prevented and an objective product can be produced in high yield. The method is thus markedly advantageous from an economical view point as a commercial process for producing oxygen-containing organic compounds such as an aromatic alcohol, a ketone, an aldehyde, a carboxylic acid and an epoxide.