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公开(公告)号：JPS61283862A
公开(公告)日：1986-12-13
申请号：JP12543485
申请日：1985-06-10
Applicant: NEC CORP
Inventor： NAKAMOTO SHINYA , KIMURA JUN
IPC: G01N27/327 , C12N11/06 , G01N27/30 , G01N27/414
Abstract: PURPOSE:To enable the formation of several kinds of enzymes at a part of a solid surface as immobilized enzyme film, by arranging a process of forming an enzyme film, a process of performing a crosslinking reaction and a process of removing an enzyme film. CONSTITUTION:A protein solution containing a first enzyme is used to form a first enzyme film 3 on the surface of a wafer in which an island-like silicon layer on the surface of a sapphire substrate 1 is used to form a sensor section 2 of an ISFET. Then, the wafer surface is covered with a metal mask 5 and a crosslinking agent solution 4 is sprayed onto the metal mask 5. Then, glutaric aldehyde solution drops are made to reach the enzyme film 3 adhered on the sensor section 2 through an opening of the metal mask 5 provided so as to be positioned on the sensor section to perform a crosslinking reaction with the enzume film 3. Then, after allowed to stand at the room temperature for about 10min., the work is washed sequentially to remove the unreacted glutaric aldehyde and all the enzyme film 3 but that immobilized on the specified sensor section 2. By this process, roughly uniform immobilized urease film can be formed at the sensor section 2.
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公开(公告)号：JPS61254845A
公开(公告)日：1986-11-12
申请号：JP9712985
申请日：1985-05-08
Applicant: NEC CORP
Inventor： KIMURA JUN , KURIYAMA TOSHIHIDE
IPC: G01N27/414 , G01N27/30 , G01N27/327
Abstract: PURPOSE:To form surely an immobilized enzyme film only to the specific part of a micro-ion sensor by providing electrodes insulated electrically from the ion sensitive part of the ion sensor near said ion sensor and forming the immobilized enzyme film on the electrodes. CONSTITUTION:The conductive electrode 7 consisting of gold is formed on ISFETs 14, 15 which are the semiconductor ion sensor and thereafter the parts thereof except the liquid contact part and the junctures of lead wires are insulated by tantalum oxide 8, by which the conductive electrodes 12, 13 covering the periphery of the respective ion sensitive parts are installed. The ion sensor element 11 having the conductive electrodes is immobilized and flocculated with the enzyme except the lead wire take-out part and thereafter the specified shape is maintained. The immobilization of the enzyme is made possible by immersing the element into water contg. collagen, etc. and applying a DC electric field between the electrodes 12 and 13.
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公开(公告)号：JPS61254844A
公开(公告)日：1986-11-12
申请号：JP9712885
申请日：1985-05-08
Applicant: NEC CORP
Inventor： KIMURA JUN , KAWANA YOSHIE , KURIYAMA TOSHIHIDE
IPC: G01N27/414 , G01N27/30
Abstract: PURPOSE:To measure the concn. of sodium having low selectivity with ionophore by two electrodes by providing two kinds of ionophore-contg. films having different sodium sensitivities to the ion sensitive parts of two ion sensitive FETs. CONSTITUTION:The respectively different ionophore films 6, 7 are formed on the two ion sensitive FETs (ISFETs) 8, 9 formed on a multi-ion sensor chip 11. The distribution coeffts. of the 1st film 6 and the 2nd film 7 with sodium and potassium are varied. The potassium concn. is determined by measuring first the output voltage E1 of the ISFET having the 1st ionophore film and the sum of the potassium concn. and the sodium concn. is determined by measuring the output voltage E2 of the ISFET in the measurable range having the 2nd ionophore film. The sodium concn. is obtd. by subtracting the same.
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公开(公告)号：JPS61245051A
公开(公告)日：1986-10-31
申请号：JP8692485
申请日：1985-04-23
Applicant: NEC CORP
Inventor： KURIYAMA TOSHIHIDE , KIMURA JUN , KAWANA YOSHIE
IPC: G01N27/00 , G01N27/30 , G01N27/414
Abstract: PURPOSE:To realize a process for producing micro-sensors which are suitable for mass-production and are made into one chip by forming enzyme immobilized films which are different from each other to the prescribed positions on the respective surfaces of semiconductor field effect type ion sensors (ISFET) made on the same chip in the stage of a wafer. CONSTITUTION:A non-water soluble high-polymer film 13 is first provided to the region except the sensor parts to be provided with the enzyme immobilized films 14 on the semiconductor wafer 3 formed with the ISFET1. Then the high- polymer soln. contg. the prescribed enzyme ejected from a nozzle 2a is stuck to the surface of the prescribed sensor parts where the film 13 is not formed to form the enzyme films. The enzyme is fixed by adding a crosslinking agent to the enzyme films or subjecting the films to photocrosslinking by which plural pieces of the different immobilized film 14 are provided to the surfaces of the prescribed sensor parts. The regions to be provided with the films 14 are controlled by the films 13 in the above-mentioned manner and therefore the accurate control of the area and thickness of the films 14 are made possible and he sensor having the uniform characteristics is obtd.
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公开(公告)号：JPS61234348A
公开(公告)日：1986-10-18
申请号：JP7575785
申请日：1985-04-10
Applicant: NEC CORP
Inventor： KAWANA YOSHIE , KIMURA JUN , KURIYAMA TOSHIHIDE
IPC: G01N27/00 , C12Q1/00 , G01N27/30 , G01N27/414
Abstract: PURPOSE:To enable massproduction of biosensors, by a method wherein an enzyme immobilized film and a photoresist layer are formed on a semiconductor wafer with an ion sensor formed thereon and then, the photoresist layer and the enzyme immobilized film are removed except for necessary portion. CONSTITUTION:An ion induction section 2 of an FE type ion sensor is provided on the top surface of a sapphire substrate 1 while a gold electrode 5 on the undersurface thereof. An enzyme immobilized film 3 and a photoresist layer 4 are formed over the entire surface on the side of the ion induction section 2. Then, a photomask is used to remove the photoresist layer 4 except for the surface of the ion induction section 2 by exposure and development and then, the work is immersed into an aqueous solution of crude trypsin or the like to decompose the enzyme-immobilized film 3. Thereafter, a biosensor with an eyzyme film at a specified ion induction section alone by removing the photoresist layer 4. Thus, a massproducible fine one-chipped sensor can be obtained by applying an IC production technology.
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Patent Agency Ranking
公开(公告)号：JPS6188135A
公开(公告)日：1986-05-06
申请号：JP20916584
申请日：1984-10-05
Applicant: Nec Corp
Inventor： KURIYAMA TOSHIHIDE , KIMURA JUN , KAWANA YOSHIE
IPC: C12Q1/00 , G01N27/00 , G01N27/414 , G01N27/30
CPC classification number: C12Q1/001 , G01N27/414
Abstract: PURPOSE:To form easily integrated micro semiconductor biosensors by integrating the plural semiconductor field effect type ion sensors on one chip. CONSTITUTION:A photoresist film 6 soluble in acetone is spin-coated on the surface of a wafer constituted by forming the semiconductor field effect type ion sensors ISFETs by using an island-shaped silicon layer on the front of a sapphire substrate 1 and depositing gold 8 by evaporation on the rear. The photoresist film on the surface of the ISFETs to be provided with an enzyme immobilizing film is removed by exposing and developing in using a photomask and thereafter a protein soln. 7 contg. enzyme and crosslinking agent is spin- coated thereon. The water is then immersed in acetone to dissolve the photoresist 6 and at the same time the enzyme immobilizing film 7 coated on the photoresist is removed. The wafer is thereafter scribed, by which the semiconductor biosensors are obtd.
Abstract translation: 目的：通过将多个半导体场效应型离子传感器集成在一个芯片上，形成容易集成的微型半导体生物传感器。 构成：将可溶于丙酮的光致抗蚀剂膜6旋涂在通过使用蓝宝石衬底1前面的岛状硅层形成半导体场效应型离子传感器ISFET的晶片的表面上，并沉积金8 通过后方蒸发。 通过使用光掩模曝光和显影，然后用蛋白质溶胶，去除要提供酶固定膜的ISFET表面上的光致抗蚀剂膜。 7对比 酶和交联剂被旋涂在其上。 然后将水浸入丙酮中以溶解光致抗蚀剂6，同时除去涂覆在光致抗蚀剂上的酶固定膜7。 然后划刻晶片，通过该晶片可以看到半导体生物传感器。
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公开(公告)号：JPS6164316A
公开(公告)日：1986-04-02
申请号：JP18763084
申请日：1984-09-07
Applicant: Nec Corp
Inventor： AWATA MASAAKI , KIMURA JUN
CPC classification number: Y02A50/2358 , Y02A50/2359
Abstract: PURPOSE: To economically treat exhaust gas while enabling the recovery of a hydrophobic org. substance, by washing exhaust gas with washing water containing bacteria to decompose a hydrophobic org. substance and subsequently containing the treated gas with an adsorbable solid to remove the remaining hydrophobic org. substance by adsorption.
CONSTITUTION: Exhaust gas 1 containing a hydrophobic org. substance and a hydrophobic org. substance is sent to a gas-liquid contact part 2 to be brought into contact with washing water 4 containing bacteria from a recirculation washing water tank 13 to perform the absorptive removal of the hydrophobic org. substance. Washing water 5 after absorption is flowed into the recirculation washing water tank 3 and all of or a part of the hydrophobic org. substance is decomposed by bacteria. The exhaust gas 6 passed through the gas-liquid contact part 2 is guided to and adsorbing part 7 packed with activated carbon to perfomm the adsorptive removal of the hydrophobic org. substance. In this case, because the hydrophobic org. substance such as phenol is already removed, the lowering in adsorptive capacity can be prevented by the covering of the surface of activated carbon with a phenol polymer. The adsorbent is desorbed by steam 9 and desorbing gas is condensed under cooling and the hydrophobic org. solvent is separated and recovered by the difference of specific gravity.
COPYRIGHT: (C)1986,JPO&JapioAbstract translation: 目的：经济地处理废气，同时能够恢复疏水组织。 通过用含有细菌的洗涤水洗涤废气以分解疏水性组分。 随后将含有可吸附固体的处理气体包含以除去剩余的疏水组织。 物质通过吸附。 构成：含有疏水组织的废气1。 物质和疏水组织。 物质被送到气液接触部分2以与来自循环洗涤水箱13的含有细菌的洗涤水4接触以进行疏水组织的吸收除去。 物质。 吸收后的洗涤水5流入再循环洗涤水箱3和疏水组织的全部或一部分。 物质被细菌分解。 通过气液接触部2的废气6被引导并吸附填充有活性炭的部分7，以对疏水组织进行吸附除去。 物质。 在这种情况下，因为疏水组织。 物质如苯酚已经被去除，通过用苯酚聚合物覆盖活性炭的表面可以防止吸附能力的降低。 吸附剂由蒸汽9解吸，解吸气在冷却下冷凝，疏水组织。 通过比重差分离和回收溶剂。
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公开(公告)号：JPS63194723A
公开(公告)日：1988-08-11
申请号：JP2583587
申请日：1987-02-05
Applicant: NEC CORP
Inventor： KAWANA YOSHIE , KIMURA JUN
Abstract: PURPOSE:To miniaturize the device and to measure a chemical substance of very small amount with high accuracy, by constituting the agitating device for very small amount of solution with a vibrator inserted into a sample solution and a piezoelectric actuator vibrating the vibrator. CONSTITUTION:The piezoelectric actuator 4 consist of an element the shape of which is changed according to the impressed voltage. When the vibrator 2 is brought in contact with the piezoelectric actuator 4 impressed with an optional pulse voltage, the optional vibration is given to the vibrator. When a top end of the vibrator 2 is inserted into the sample solution 3, the solution is efficiently agitated by the vibration of the vibrator. The agitation is carried out in every case where the solution is contained in a microvessel, where a flow cell is used in a closed flow, or where a solution drop is put on a hydrophobic base plate.
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公开(公告)号：JPS6368085A
公开(公告)日：1988-03-26
申请号：JP21019386
申请日：1986-09-05
Applicant: NEC CORP
Inventor： MACHIDA NOBUYUKI , KIMURA JUN
Abstract: PURPOSE:To obtain an immobilized microbial cell, etc., having improved activity, strength, etc., readily, by spraying alginic acid gel containing a microbial cell or an enzyme upon a mixed solution of acrylamide and a gelatinizing agent to form fine gel and gelatinizing the mixed solution. CONSTITUTION:An acrylamidemonomer 4 is mixed with an alginic acid gelatinizing agent 5 (e.g. calcium chloride) by a monomer blender 6 and the prepared monomer mixed solution is sent to a spray chamber 7. Separately, a concentrated microbial cell solution or enzyme solution 2 is blended with an aqueous solution 1 of alginic acid by a blender 3 and the prepared mixed sol is sprayed upon the monomer mixed solution in the spray chamber 7 to form fine alginic acid gel. Then the monomer mixed solution containing the fine alginic acid gel is sent to an immobilizing device 8 and the acryl amide is polymerized. Consequently, the fine alginic acid immobilized cell or enzyme is dispersed into and maintained in the acrylamide gel matrix to give the aimed immobilized microbial cell or enzyme.
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公开(公告)号：JPS61216717A
公开(公告)日：1986-09-26
申请号：JP5783585
申请日：1985-03-22
Applicant: NEC CORP
Inventor： MACHIDA NOBUYUKI , KIMURA JUN
Abstract: PURPOSE:To economically treat org. waste gas by utilizing bacteria, by partially immersing a rotor, wherein discs and radial blades are combined, in washing water and sending org. waste gas into washing water as fine gas bubbles from the part below the rotor. CONSTITUTION:Org. waste gas containing phenol and acetic acid etc. is introduced into a contact reaction tank 5 under pressure from a feed-in port 1 to be diffused as fine gas bubbles from the gas diffusion plate 6 provided to the bottom part of said reaction tank 5. The waste gas rises while receives the gas-liquid contact treatment with washing water and the power of the motor 9 for rotating a rotor A, wherein discs 7 and blades 8 are alternately arranged, is reduced by the buoyancy of said gas. The org. components in the waste gas are transferred to washing water and decomposed by the bacteria adhered to the discs 7 at high concn. and suspended bacteria in the washing water. Necessary oxygen is supplied by exposing the rotating discs 7 to air and by diffusing waste gas as fine gas bubbles. The bacteria adhered to the discs 7 are released properly by vibration due to gas bubbles to stably hold bacteria membranes.
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