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    LIPASE VARIANT
    5.
    发明公开
    LIPASE VARIANT 有权
    脂肪酶

    公开(公告)号：EP1054956A1

    公开(公告)日：2000-11-29

    申请号：EP99934304.9

    申请日：1999-02-17

    Applicant: NOVO NORDISK A/S

    Inventor： BORCH, Kim ,  VIND, Jesper ,  SVENDSEN, Allan ,  PETERSEN, Dorte, Aaby ,  PATKAR, Shamkant, Anant ,  BOJSEN, Kirsten

    IPC: C12N9/20 ,  C11D3/386

    CPC classification number: C11D1/83 ,  C11D1/02 ,  C11D3/38627 ,  C12N9/20

    Abstract: Variants of Lipolase® (wild-type Humicola lanuginosa lipase) with a certain distribution of electrically charged amino acids have a particularly good first-wash performance in a detergent solution with a high ratio of anionic to non-ionic surfactant. The effect is achieved by attaching a positively charged peptide extension at the N-terminal and by imposing certain restrictions on the charge distribution in the region corresponding to amino acid positions 90-101 and at position 210. The inventors further devised a method of developing variants with such performance from Lipolase by attaching a peptide extension at the N-terminal and substituting amino acids in the region 90-101 or in the immediate surroundings in the three-dimensional structure. The lipases may further provide additional benefits, such as whiteness maintenance and dingy cleanup.

    C. ANTARCTICA LIPASE AND LIPASE VARIANTS
    8.
    发明公开
    C. ANTARCTICA LIPASE AND LIPASE VARIANTS 失效
    C. ANTARTICA-LIPASE UND -IPASEVARIANTEN。

    公开(公告)号：EP0652945A1

    公开(公告)日：1995-05-17

    申请号：EP93914647.0

    申请日：1993-07-05

    Applicant: NOVO NORDISK A/S

    Inventor： SVENDSEN, Allan ,  PATHAR, Shamkant, Anant ,  EGEL-MITANI, Michi ,  BORCH, Kim ,  CLAUSEN, Ib, Groth ,  HANSEN, Mogens, Trier

    IPC: C12N15 ,  C12N1 ,  C12N9 ,  C12P7 ,  C12S3 ,  D21C9 ,  D21H21 ,  C12R1

    CPC classification number: D21H21/02 ,  C07K2299/00 ,  C12N9/20

    Abstract: A lipase variant of a parent lipase comprising a trypsin-like catalytic triad including an active serine located in a predominantly hydrophobic, elongated binding pocket of the lipase molecule and, located in a critical position of a lipid contact zone of the lipase structure, an amino acid residue different from an aromatic amino acid residue, which amino acid residue interacts with a lipid substrate at or during hydrolysis, in which lipase variant said amino acid residue has been replaced by an aromatic amino acid residue so as to confer to the variant an increased specific activity as compared to that of the parent lipase. The parent lipase may be a C. antarctica lipase A essentially free from other substances from C. antarctica, which comprises the amino acid sequence shown in SEQ ID No. 2, or a variant of said lipase which (1) has lipase activity, (2) reacts with an antibody reactive with at least one epitope of C. antarctica lipase A having th eamino acid sequence SEQ ID No. 2, and/or (3) is encoded by a nucleotide sequence which hybridizes with an oligonucleotide probe prepared on the basis of the full or partial nucleotide sequence shown in SEQ ID No. 1 encoding the C. antarctica lipase A.

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