中科微点-全球专利检索

  1. Login
  2. Sign Up

Search Results

6 records (took 0.027 seconds)

    AN $i(IN VITRO) METHOD FOR CONSTRUCTION OF A DNA LIBRARY
    2.
    发明公开
    AN $i(IN VITRO) METHOD FOR CONSTRUCTION OF A DNA LIBRARY 失效
    为了制备DNA文库的体外方法

    公开(公告)号：EP0973940A1

    公开(公告)日：2000-01-26

    申请号：EP98907911.6

    申请日：1998-03-18

    Applicant: NOVO NORDISK A/S

    Inventor： VIND, Jesper

    IPC: C12Q1/68 ,  C12N15/10

    CPC classification number: C12N15/1093 ,  C12N15/1027 ,  C12Q1/6811

    Abstract: A method for in vitro construction of a library of recombined homologous polynucleotides from a number of different starting DNA templates and primers by induced template shifts during a polynucleotide synthesis is described, whereby: A. extended primers are synthesized by a) denaturing the DNA templates, b) annealing primers to the templates, c) extending the said primers by use of a polymerase, d) stop the synthesis, and e) separate the extended primers from the templates; B. a template shift is induced by a) isolating the extended primers from the templates and repeating steps A.b) to A.e) using the extended primers as both primers and templates, or b) repeating steps A.b) to A.e), C. this process is terminated after an appropriate number of cycles of process steps A. and B.a), A. and B.b), or combinations thereof. Optionally the polynucleotides are amplified in a a standard PCR reaction with specific primers to selectively amplify homologous polynucleotides of interest.

    LIPASE VARIANT
    3.
    发明公开
    LIPASE VARIANT 有权
    脂肪酶

    公开(公告)号：EP1054956A1

    公开(公告)日：2000-11-29

    申请号：EP99934304.9

    申请日：1999-02-17

    Applicant: NOVO NORDISK A/S

    Inventor： BORCH, Kim ,  VIND, Jesper ,  SVENDSEN, Allan ,  PETERSEN, Dorte, Aaby ,  PATKAR, Shamkant, Anant ,  BOJSEN, Kirsten

    IPC: C12N9/20 ,  C11D3/386

    CPC classification number: C11D1/83 ,  C11D1/02 ,  C11D3/38627 ,  C12N9/20

    Abstract: Variants of Lipolase® (wild-type Humicola lanuginosa lipase) with a certain distribution of electrically charged amino acids have a particularly good first-wash performance in a detergent solution with a high ratio of anionic to non-ionic surfactant. The effect is achieved by attaching a positively charged peptide extension at the N-terminal and by imposing certain restrictions on the charge distribution in the region corresponding to amino acid positions 90-101 and at position 210. The inventors further devised a method of developing variants with such performance from Lipolase by attaching a peptide extension at the N-terminal and substituting amino acids in the region 90-101 or in the immediate surroundings in the three-dimensional structure. The lipases may further provide additional benefits, such as whiteness maintenance and dingy cleanup.

Patent Agency Ranking