公开(公告)号：EP1865315A4

公开(公告)日：2017-12-06

申请号：EP06730437

申请日：2006-03-29

Applicant: OLYMPUS CORP

Inventor： MATSUO YUICHIRO ,  TAKAGI KOSUKE ,  SHIMADA YOSHIHIRO ,  KAWAI TSUYOSHI

IPC: G01N33/48

CPC classification number: G01N33/5026 ,  G01N15/1475 ,  G01N21/6458 ,  G01N33/5008 ,  G06K9/0014 ,  G06T7/0012 ,  G06T2207/30024

Abstract: In compound screening using cell images, compounds having an effective action on cells are searched for by automated processing, to more perform existing methods for compound searching more effectively. There is provided an apparatus including an image-acquisition unit (55) for capturing a plurality of cell images under different image-acquisition conditions; and an analysis unit (80) for extracting a cell position and at least one feature on the basis of the plurality of cell images and for performing statistical analysis with the extracted features serving as parameters.

公开(公告)号：EP1972982A4

公开(公告)日：2011-01-05

申请号：EP07706643

申请日：2007-01-12

Applicant: OLYMPUS CORP

Inventor： MATSUO YUICHIRO

IPC: G02B21/00 ,  G02B7/28

CPC classification number: G02B21/245

公开(公告)号：EP1669757A4

公开(公告)日：2007-03-28

申请号：EP04772790

申请日：2004-09-03

Applicant: OLYMPUS CORP

Inventor： MATSUO YUICHIRO

IPC: B01L3/00 ,  G01N33/53 ,  B01L7/00 ,  C12M1/34 ,  C40B60/14 ,  G01N37/00

CPC classification number: B01L3/50273 ,  B01J2219/00477 ,  B01J2219/00693 ,  B01J2219/00698 ,  B01L7/00 ,  B01L2200/146 ,  B01L2300/0819 ,  B01L2300/14 ,  B01L2300/1827 ,  B01L2400/0487 ,  C40B60/14

Abstract: A system for reacting a biological material comprises an incubator (200) for housing and holding a reaction container (100), a pump (600) for driving a reaction liquid containing a biological material in the reaction container (100), a reaction liquid driving tube (400) for transmitting changes in pressure from the pump (600) to the reaction liquid, and a pressure sensor (500) for sensing the pressure in the reaction liquid driving tube (400).

公开(公告)号：JP2012019748A

公开(公告)日：2012-02-02

申请号：JP2010160820

申请日：2010-07-15

Applicant: Olympus Corp ,  オリンパス株式会社

Inventor： MATSUO YUICHIRO

IPC: C12M1/34 ,  C12Q1/02

CPC classification number: G02B21/365

Abstract: PROBLEM TO BE SOLVED: To provide a cell observation apparatus and an observation method with which only an observation target cell in an region of interest is illuminated with illuminating light to prevent photobleaching of cells that are not the observation target.SOLUTION: The cell observation apparatus includes an objective lens that illuminates a specimen with illuminating light, a scanner that adjusts an area illuminated with illuminating light by the objective lens, an image generating portion 31 that generates an image of the specimen illuminated by the objective lens, a cell-data extracting portion 32 that extracts data showing the characteristics of each cell from the generated image of the specimen, a cell-area extracting portion 33 that extracts an area of each cell associated with the extracted data of each cell, a cell-of-interest selecting portion 35 that selects the cell of interest from the specimen in accordance with the extracted data of each cell, and a controller that operates the scanner to illuminate the cell area corresponding to the cell of interest selected by the cell-of-interest selecting portion 35 with the illuminating light.

Abstract translation: 解决的问题：提供一种细胞观察装置和观察方法，其中仅用感兴趣的区域中的观察目标细胞用照明光照射，以防止不是观察对象的细胞的漂白。 解决方案：细胞观察装置包括用照明光照射样本的物镜，调节由物镜照射光照射的区域的扫描仪，生成被照射的样本的图像的图像生成部31 物镜，细胞数据提取部分32，从生成的样本图像中提取表示每个细胞的特征的数据;细胞区域提取部分33，提取与每个细胞的提取数据相关联的每个细胞的区域 ，感兴趣细胞选择部分35，其根据每个细胞的提取数据从样本中选择感兴趣的细胞;以及控制器，其操作扫描仪以照亮与由所述细胞所选择的感兴趣的细胞相对应的细胞区域 感兴趣的感光体选择部分35具有照明光。 版权所有（C）2012，JPO＆INPIT

公开(公告)号：JP2010216920A

公开(公告)日：2010-09-30

申请号：JP2009062608

申请日：2009-03-16

Applicant: Olympus Corp ,  オリンパス株式会社

Inventor： AMAKAWA GENTA ,  TAKAGI KOSUKE ,  MATSUO YUICHIRO

IPC: G01N33/48 ,  C12M1/34

CPC classification number: G06K9/00134

Abstract: PROBLEM TO BE SOLVED: To provide a cell image analyzing appparatus capable of accurately and automatically recognizing individual cells in undyed bright-field observation and capable of sharply reducing the physical load and treatment time of an analyzer, in cell analysis.
SOLUTION: The cell image analyzer has a cell imaging means 1, having an imaging optical system 1a and an imaging element 1b and imaging the cells present in a container and a cell image analyzing means 2 for automatically analyzing the predetermined feature quantity related to the cells while specifying a cell region, by using the images of the cells imaged through the cell imaging means 1 and further has a cell contour enhancement means 3 for automatically emphasizing the contour parts of the images of the cells existing inside the container which is arranged at the imaging position of the cell imaging means.
COPYRIGHT: (C)2010,JPO&INPIT

Abstract translation: 要解决的问题：在细胞分析中，提供能够精确自动地识别未染色的光场观察中的各个细胞并能够大幅度降低分析仪的物理负荷和治疗时间的细胞图像分析装置。 解决方案：细胞图像分析仪具有细胞成像装置1，具有成像光学系统1a和成像元件1b，并且对存在于容器中的细胞进行成像和细胞图像分析装置2，用于自动分析相关的预定特征量 通过使用通过细胞成像装置1成像的细胞的图像，同时指定细胞区域，并且还具有用于自动强调存在于容器内的细胞的图像的轮廓部分的细胞轮廓增强装置3，所述细胞轮廓增强装置3是 布置在细胞成像装置的成像位置。 版权所有（C）2010，JPO＆INPIT

公开(公告)号：JP2008064534A

公开(公告)日：2008-03-21

申请号：JP2006241312

申请日：2006-09-06

Applicant: Olympus Corp ,  オリンパス株式会社

Inventor： TAKAGI KOSUKE ,  SHIMADA YOSHIHIRO ,  MATSUO YUICHIRO

IPC: G01N33/48 ,  C12M1/34 ,  G06T1/00

CPC classification number: G01N15/1463 ,  G06K9/0014 ,  G06T7/0012 ,  G06T2207/30024

Abstract: PROBLEM TO BE SOLVED: To separate and extract a cell showing specific properties from noise by processing a cell image to analyze the same.
SOLUTION: This cell image processor 1 is equipped with a feature quantity measuring part 11 for processing the cell image acquired by photographing the cells and measuring the feature quantities of the cells contained in the cell image, a feature quantity distribution forming part 12 for forming the distribution of the measured feature quantities, a group forming part 13 for grouping the cells having the continuously distributed feature quantities in the formed distribution of the feature quantities and a specific cell extraction part 14 for extracting the cell having a predetermined range of the feature quantities at both ends of distribution of the feature quantities of the respective groups as a specific cell.
COPYRIGHT: (C)2008,JPO&INPIT

Abstract translation: 要解决的问题：通过处理细胞图像来分析并提取显示特定性质的细胞从噪声中分析。 解决方案：该单元图像处理器1配备有特征量测量部11，用于处理通过拍摄单元获取的单元图像并测量单元图像中包含的单元的特征量，特征量分布形成部12 为了形成所测量的特征量的分布，组形成部分13用于将具有连续分布特征量的细胞分组在特征量的形成分布中，以及特定细胞提取部分14，用于提取具有预定范围的特征量的细胞 作为特定单元的各组的特征量的分布的两端的特征量。 版权所有（C）2008，JPO＆INPIT

公开(公告)号：JP2004279258A

公开(公告)日：2004-10-07

申请号：JP2003072147

申请日：2003-03-17

Applicant: Olympus Corp ,  オリンパス株式会社

Inventor： MATSUO YUICHIRO ,  SHIBAZAKI TAKAMI

IPC: G01N21/64

Abstract: PROBLEM TO BE SOLVED: To provide a light measurement apparatus and a method capable of always, reliably and accurately measuring a light in an optimal state.
SOLUTION: As an optical transmittance of a ND (nutral density) filter 17 is changed based on a comparison result of an output from a photo multiplier 13 in response to fluorescence intensity from a spot (a probe) of a micro array chip 7 and a reference, the output from the photo multiplier 13 is always and regularly controlled and corrected by a fluorescence intensity conversion process based on information on the optical transmittance of the ND filter 17.
COPYRIGHT: (C)2005,JPO&NCIPI

公开(公告)号：JP2010216919A

公开(公告)日：2010-09-30

申请号：JP2009062607

申请日：2009-03-16

Applicant: Olympus Corp ,  オリンパス株式会社

Inventor： TSUTSUI AKIRA ,  MATSUO YUICHIRO ,  SHIMADA YOSHIHIRO

IPC: G01N21/17 ,  C12M1/34 ,  G01N21/64

Abstract: PROBLEM TO BE SOLVED: To provide an image pickup device for certainly extracting a target signal from all of imaging target regions at a high speed to perform detailed analysis, not becoming large-scale and for being reduced in cost, and a cell image analyzing system equipped with the same. SOLUTION: The electron multiplying gain of a cooling CCD camera 4 is made high, the resolving power of the cooling CCD camera is made low and the exposure time of a light source 3 is made short with respect to all of the imaging target regions in a container 1 to perform imaging in a high throughput mode and the target signal is extracted from an image group picked up in the high throughput mode on the basis of a predetermined extraction condition. Subsequently, the electron multiplying gain of the cooling CCD camera is made low, the resolving power of the cooling CCD camera is made high and the exposure time of the light source is made long only with respect to the imaging target regions corresponding to the region where the target signal in the image, wherein the target signal is extracted in the container, to perform imaging in a highly detailed mode. COPYRIGHT: (C)2010,JPO&INPIT

Abstract translation: 要解决的问题：提供一种用于从所有成像目标区域高速提取目标信号以进行详细分析，不会变得大规模并且降低成本的图像拾取装置和单元 图像分析系统配备相同。 解决方案：使冷却CCD摄像机4的电子倍增增益变高，冷却CCD摄像机的分辨率降低，光源3的曝光时间相对于所有成像目标变短 容器1中的区域，以高通量模式进行成像，并且基于预定的提取条件从在高通量模式中拾取的图像组中提取目标信号。 随后，冷却CCD摄像机的电子倍增增益变低，使得冷却CCD摄像机的分辨率高，并且光源的曝光时间只对相对于与 图像中的目标信号，其中在容器中提取目标信号，以高度详细的模式进行成像。 版权所有（C）2010，JPO＆INPIT

公开(公告)号：JP2008046305A

公开(公告)日：2008-02-28

申请号：JP2006221108

申请日：2006-08-14

Applicant: Olympus Corp ,  オリンパス株式会社

Inventor： TAKAGI KOSUKE ,  MATSUO YUICHIRO ,  SHIMADA YOSHIHIRO

IPC: G02B7/28 ,  G02B7/36 ,  G02B21/00 ,  H04N5/232

CPC classification number: G02B21/244 ,  G01N21/6458 ,  H04N5/23212

Abstract: PROBLEM TO BE SOLVED: To provide an automatic focusing device where, in the acquisition of the image of a cell, a focal position is correctly set to a cell having high activity without adjusting the focus on a dead cell.
SOLUTION: The automatic focusing device 8 used for a microscope 1 where fluorescence emitted from a cell is imaged to acquire a cell image, is provided with: a setting means 5 for setting a luminance range showing the presence region of a living cell on the basis of the luminance distribution of the acquired cell image; and a focusing detection means 5 for detecting the focal position on the basis of luminance of the image of the nuclei of the cell in the luminance range set by the setting means 5.
COPYRIGHT: (C)2008,JPO&INPIT

Abstract translation: 要解决的问题：提供一种自动对焦装置，其中，在获取单元的图像时，将焦点位置正确地设置为具有高活动性的单元，而不调整对死细胞的聚焦。 解决方案：用于显微镜1的自动聚焦装置8，其中从单元发射的荧光成像以获取细胞图像，设置有：设定装置5，用于设定表示活细胞存在区域的亮度范围 基于所获取的小区图像的亮度分布; 以及聚焦检测装置5，用于基于由设置装置5设置的亮度范围中的单元的核的图像的亮度来检测焦点位置。（C）2008年，JPO和INPIT

公开(公告)号：JP2008015074A

公开(公告)日：2008-01-24

申请号：JP2006184210

申请日：2006-07-04

Applicant: Olympus Corp ,  オリンパス株式会社

Inventor： MATSUO YUICHIRO

IPC: G02B7/28 ,  G02B21/00

Abstract: PROBLEM TO BE SOLVED: To accurately perform high-speed processing for autofocus with simple constitution.
SOLUTION: The autofocus device 1C is provided and comprises: a detection means 2C having a laser diode 13 and a photodetector 18 detecting reflected light which is emitted from the laser diode 13, and reflected on the surface and the boundary surface of a microtiter plate 12 and returning therefrom through an objective lens 2; a focal plane moving means 33 adjusting the relative position of the focal plane of the objective lens 2 to an object to be inspected in an optical axis direction; a control means 19 determinding whether the focal plane of the objective 2 is aligned with the surface or the boundary surface of the microtiter plate 12 based on output from the photodetector 18 and controlling the focal plane moving means 33 based on the result of determination; and a gain adjusting means switching the input gain and/or the output gain of the detection means 2C between when detecting the surface of the microtiter plate 12 and when detecting the boundary surface thereof.
COPYRIGHT: (C)2008,JPO&INPIT

Abstract translation: 要解决的问题：以简单的结构精确地执行自动对焦的高速处理。 解决方案：提供自动对焦装置1C，其包括：检测装置2C，具有激光二极管13和检测从激光二极管13发出的反射光的光电检测器18，并在其表面和边界面上反射 微量滴定板12并通过物镜2返回; 焦平面移动装置33，调整物镜2的焦平面相对于被检体的光轴方向的相对位置; 控制装置19基于光电检测器18的输出确定物镜2的焦平面是否与微量滴定板12的表面或边界表面对准，并基于确定结果控制焦平面移动装置33; 以及增益调整装置，当检测微量滴定板12的表面和检测其边界表面时，切换检测装置2C的输入增益和/或输出增益。 版权所有（C）2008，JPO＆INPIT